Beneficial effect and potential molecular mechanism of chloroquine on sperm motility and fertilizing ability in yellow catfish

Jin, Zhang; Ma, Wuhua; Xie, Binyue; Gui, Jian‐Fang; Mei, Jie

doi:10.1016/j.aquaculture.2016.10.028

Cited by 18 publications

(17 citation statements)

References 35 publications

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“…Autophagy, a natural and physiological process, plays important roles in gamete maturation and fertilization [ 11 , 12 ]. In addition, administration of chloroquine, a well-known autophagy inhibitor, could increase sperm motility and fertilization rate, with the activation of PI3K-AKT signaling pathway [ 14 – 16 ]. In the current study, we found that leucine can effectively improve sperm motility by promoting autophagosome formation and inhibiting fusion of autophagosome and lysosome, depending on the activation of PI3K/AKT pathway (Figure 8 ).…”

Section: Discussionmentioning

confidence: 99%

“…Autophagy-related protein 7 (Atg7) is essential for mouse acrosome biogenesis during spermatogenesis, depletion of which results in complete infertility with aberrant acrosome formation [ 13 ]. Administration of Chloroquine, a well-known autophagy inhibitor, can increase the sperm motility and fertilization in mammals and fish via activation of PI3K-AKT signaling pathway [ 14 – 16 ]. Until now, it is unknown that whether some essential amino acids are involved in the regulation of zebrafish sperm motility.…”

Section: Introductionmentioning

confidence: 99%

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Leucine mediates autophagosome-lysosome fusion and improves sperm motility by activating the PI3K/Akt pathway

Zhang

Liu

et al. 2017

Oncotarget

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Amino acid supplementation is an efficient and effective strategy to increase sperm quality. In our research, a comparative study was conducted to screen free amino acids to improve sperm motility, and we found that leucine was the most efficient one. Leucine treatment increases sperm motility depending on the activation of PI3K/Akt signaling pathway, while the chemical inhibitor of PI3K/Akt signal could reduce the amount of pAkt activated by leucine treatment. Moreover, leucine treatment improved the expression of P62 and LC3-II, substantially suppressed the autophagy process in zebrafish testis. In vitro studies showed that leucine could reduce the fusion of autophagosome and lysosome that was indicated by the co-localization of EGFP-LC3 and lysosome marker. Two chemical modulators of autophagy, such as LY294002 (the inhibitor of PI3K/Akt signal) and chloroquine were administered to investigate the process of autophagy on zebrafish sperm motility. LY294002 inhibited autophagosome formation to reduced sperm motility, while chloroquine inhibited the fusion of autophagosome and lysosome to improve sperm motility. Our data suggest that short-term treatment with leucine could increase zebrafish sperm motility by affecting the autophagy and inhibiting the fusion of autophagosome and lysosomes, depending on the activation of PI3K/Akt signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Leucine mediates autophagosome-lysosome fusion and improves sperm motility by activating the PI3K/Akt pathway

Zhang

Liu

et al. 2017

Oncotarget

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“…Sperm samples of the same age from both the control and the F3 generation homozygous fish were kept under the same culture conditions and stored in preservative fluid (63 mM NaCl, 19 mM KCl, 1.3 mM CaCl 2 , 4.7 mM MgSO 4 ·7H 2 O, 2.5 mM NaHCO 3 , pH 7.4). Parameters of sperm motility were determined by a computer-assisted sperm analysis (CASA) system (Hamilton-Thorne Biosciences, Beverly, MA, USA) [ 55 ]. Immediately after activation in room temperature (25 ± 1 °C), sperm motility was evaluated under 4× magnifications after adjusting the number of sperm from 200 to 300 in a microscope field.…”

Section: Methodsmentioning

confidence: 99%

miR-34a Regulates Sperm Motility in Zebrafish

Guo

Xie

Xiong

et al. 2017

IJMS

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Increasing attention has been focused on the role of microRNAs in post-transcription regulation during spermatogenesis. Recently, the miR-34 family has been shown to be involved in the spermatogenesis, but the clear function of the miR-34 family in spermatogenesis is still obscure. Here we analyzed the function of miR-34a, a member of the miR-34 family, during spermatogenesis using miR-34a knockout zebrafish generated by the clustered regularly interspaced short palindromic repeats/associated protein 9 (CRISPR/Cas9) system. miR-34a knockout zebrafish showed no obvious defects on testis morphology and sperm quantity. However, we found a significant increase in progressive sperm motility that is one of the pivotal factors influencing in vitro fertilization rates, in the knockout zebrafish. Moreover, breeding experiments showed that, when miR-34a-knockout male zebrafish mated with the wide-type females, they had a higher fertilization rate than did the wide-type males. Glycogen synthase kinase-3a (gsk3a), a potential sperm motility regulatory gene was predicted to be targeted by miR-34a, which was further supported by luciferase reporter assays, since a significant decrease of luciferase activity was detected upon ectopic overexpression of miR-34a. Our findings suggest that miR-34a downregulates gsk3a by targeting its 3′ untranslated region, and miR-34a/gsk3a interaction modulates sperm motility in zebrafish. This study will help in understanding in the role of the miR-34 family during spermatogenesis and will set paths for further studies.

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“…A nivel genético, se presenta evidencia del efecto mutagénico de la CQ en Drosophila melanogaster (6) , roturas del ADN en células de hígado de ratas (7) , roturas cromosómicas en células de médula ósea y alteraciones en la morfología de los espermatozoides en Mesocricetus auratus (8) . Contrariamente a estos reportes, en el bagre amarillo se observó que la CQ mejora la motilidad de los espermatozoides e incrementa su capacidad de fertilización (9) . El uso frecuente de la CQ para distintas patologías, el bajo costo, el reporte de efectos secundarios, el periodo de tratamiento de tres a cuatro meses, el alto grado de Amésquita L et al…”

Section: Introductionunclassified

Daño en el ADN de linfocitos humanos por efecto de cloroquina

Amésquita

Cruz-Briceño

Prieto

2018

Rev Peru Med Exp Salud Publica

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Citar como: Amésquita L, Cruz-Briceño MN, Prieto Z. Daño en el ADN de linfocitos humanos por efecto de cloroquina. Rev Peru Med Exp Salud Publica. 2018;35(3):471-5. RESUMENEl objetivo del estudio fue evaluar el efecto de cloroquina (CQ) en linfocitos humanos a través del ensayo cometa. Los linfocitos fueron aislados de muestras de sangre periférica obtenidas de tres donantes sanos, no fumadores de 24 a 30 años. Los linfocitos aislados fueron expuestos durante una hora a diversos tratamientos: peróxido de hidrógeno 2,5 % (control positivo), buffer fosfato salino 1X (control negativo) y cloroquina a concentraciones de 0 µg/ml, 0,25 µg/ml; 5 µg/ml y 300 µg/ml. Se registró los promedios del porcentaje de ADN en la cola del cometa, momento de la cola y momento de la cola de Olive, encontrándose diferencias significativas entre las diversas concentraciones de CQ (p<0,01). Asimismo, la magnitud de daño del ADN se incrementó en función de la concentración de CQ. Se demostró el efecto genotóxico de CQ en linfocitos humanos expuestos in vitro. ABSTRACTThe objective of the study was to evaluate the effect of chloroquine (CQ) on human lymphocytes through the Comet Assay. Lymphocytes were isolated from peripheral blood samples obtained from three healthy, non-smoking donors aged 24 to 30 years. The isolated lymphocytes were exposed for one hour to various treatments: hydrogen peroxide 2.5% (positive control), saline buffer phosphate 1X (negative control) and chloroquine at concentrations of 0 µg/ml, 0.25 µg/ml; 5 µg/ml and 300 µg/ml. The averages of the percentage of DNA in the comet tail, moment of tail and moment of Olive's tail were recorded, with significant differences between the different concentrations of CQ (p<0.01). Also, the magnitude of DNA damage was increased as a function of the CQ concentration. The genotoxic effect of CQ was demonstrated in human lymphocytes exposed in vitro.

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Beneficial effect and potential molecular mechanism of chloroquine on sperm motility and fertilizing ability in yellow catfish

Cited by 18 publications

References 35 publications

Leucine mediates autophagosome-lysosome fusion and improves sperm motility by activating the PI3K/Akt pathway

Leucine mediates autophagosome-lysosome fusion and improves sperm motility by activating the PI3K/Akt pathway

miR-34a Regulates Sperm Motility in Zebrafish

Daño en el ADN de linfocitos humanos por efecto de cloroquina

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