Stimulation of wound healing, using brain extract with fibroblast growth factor (FGF) activity

Buntrock, P; Jentzsch, K.D.; Heder, G.

doi:10.1016/s0232-1513(82)80051-0

Cited by 55 publications

(10 citation statements)

References 7 publications

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“…In addition, bFGF is reported to have a variety of biological activities [19][20][21] and to be effective in enhancing wound healing through induction of neovascularization [22,23] and regeneration of bone [24,25], cartilage [26,27] and nerve [28,29], when administrated in the form of a solution. The most important of concern regarding the delivery of proteins is whether or not the protein released in the body actually retains its biological activity.…”

Section: Discussionmentioning

confidence: 99%

Enhanced angiogenesis through controlled release of basic fibroblast growth factor from peptide amphiphile for tissue regeneration

et al. 2006

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Section: Discussionmentioning

confidence: 99%

Enhanced angiogenesis through controlled release of basic fibroblast growth factor from peptide amphiphile for tissue regeneration

et al. 2006

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“…The mitogenic effect is mediated by receptors . In vivo, bFGF induces differentiation of cartilage and bone (Wellmitz et al 1980) and increases the formation of granulation tissue during the course of wound healing (Buntrock et al 1984). Many studies on in vivo effects have been concerned with the angiogenic activity of bFGF, and it has been found to be active in the rabbit cornea, the hamster cheek pouch, and the 9 day CAM (Gospodarowicz et al 1979(Gospodarowicz et al , 1984(Gospodarowicz et al , 1985.…”

Section: Effects Of Bfgfmentioning

confidence: 99%

A modified chorioallantoic membrane (CAM) assay for qualitative and quantitative study of growth factors

1991

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The application of Thermanox tissue culture coverslips to the day 9 CAM of the chick causes constant effects beneath the carrier after 3 days, and these are associated with a change in the blood vessel pattern. Histological sections show enormous thickening of the CAM in the reactive areas. The stroma of the CAM shows fibrocyte proliferation, leucocyte infiltration, and clusters of dispersed ectodermal epithelial cells exhibiting signs of necrosis. The latter obviously cause a strong vascular response. The same effects are seen when the Thermanox discs are applied at day 11. Following application on day 12 a positive or negative response to the carrier is observed, whereas on day 13 no such carrier effects are seen. The only remaining effect is compression of the intra-ectodermal capillary plexus of the CAM. This can macroscopically be seen after peroxidase staining of the blood vessels. The effect of 5 microliters PBS dried on the Thermanox disc and applied to the day 13 CAM is to cause, after 3 days, hyperosmotic damage to the ectodermal epithelium, which becomes overgrown by fibrocytes. We found dose-dependent effects of salt-free human bFGF applied to the day 13 CAM. The first and main effect is fibrocyte proliferation (0.5 microgram). New capillaries appear with higher doses, but are not as frequent as would be expected for an angiogenic substance (1.25-2.5 micrograms). Also with higher doses additional hyperplasia of the endodermal (3.75 micrograms) and ectodermal (5 micrograms) epithelium can be seen. The latter might be a non-specific hyperosmotic effect. Leucocytes are regularly present within the reactive areas. When salt-free angiogenin is applied to the day 13 CAM, some effects appear with doses of 4.6 micrograms and more. The ectodermal epithelium of the reactive areas is discontinuous, exhibiting signs of necrosis. It is overgrown by parallel fibrocytes. Whether this is a non-specific hyperosmotic effect, or indicates enhancement of invasive growth, calls for further investigation.

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“…Fibroblasts usually surround granuloma, and play an important role in wound healing. These processes are mediated by growth factors and cytokines, including platelet-derived growth factors [2], transforming growth factor b [3], fibroblast growth factors [4][5][6], and connective tissue growth factor [7]. We have purified and identified S100A9 as a new fibroblast growthstimulating factor (FGSF) from the exudate of carrageenaninduced granulomatous inflammation in rats in this study.…”

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Fibroblast growth‐stimulating activity of S100A9 (MRP‐14)

Shibata

Miyama

Shinoda

et al. 2004

European Journal of Biochemistry

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Fibroblasts play a critical role in chronic inflammation and wound healing. In this study, a fibroblast growthstimulating factor was purified from the exudate of carrageenan-induced inflammation in rats. The purified protein was a disulfide-linked homodimer. Amino acid sequence analysis of the peptides generated by cleavage with cyanogen bromide and proteinase V8 resulted in identification of the protein as S100A9. Recombinant S100A9 as well as its disulfide-linked homodimer stimulated the proliferation of fibroblasts at a similar concentration of the purified protein. The concentration of S100A9 in the exudate was determined by immunoblot analysis. The total protein concentration in the exudate reached a maximum 4 days after carrageenan injection and then slightly decreased, whereas the concentration of S100A9 reached a maximum at day 3 and then decreased rapidly. These studies show that S100A9 is present at a high concentration in the exudate of carrageenan-induced inflammation in rats, and that S100A9 stimulates proliferation of fibroblasts, suggesting that it plays a role in chronic inflammation.

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Stimulation of wound healing, using brain extract with fibroblast growth factor (FGF) activity

Cited by 55 publications

References 7 publications

Enhanced angiogenesis through controlled release of basic fibroblast growth factor from peptide amphiphile for tissue regeneration

Enhanced angiogenesis through controlled release of basic fibroblast growth factor from peptide amphiphile for tissue regeneration

A modified chorioallantoic membrane (CAM) assay for qualitative and quantitative study of growth factors

Fibroblast growth‐stimulating activity of S100A9 (MRP‐14)

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