Enhanced angiogenesis through controlled release of basic fibroblast growth factor from peptide amphiphile for tissue regeneration

Hosseinkhani, Hossein; Hosseinkhani, Mohsen; Khademhosseini, Ali; Kobayashi, Haruo; Tabata, Yasuhiko

doi:10.1016/j.biomaterials.2006.08.003

Cited by 180 publications

(134 citation statements)

References 29 publications

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“…When the cell cultures reached 80% confluence they were harvested by trypsinization with an aqueous solution of 0.05 wt% trypsin (Gibco) and 1 Cell seeding into 3D collagen-PGA nanofiber sheets and proliferation assay MSC were seeded into nanofiber sheets according to an agitated seeding method as this method was effective in seeding cells homogenously into porous 3D cell scaffolds. 22 Briefly, 0.5 mL of cell suspension (1 × 10 6 cells/mL) and the nanofiber sheets were placed in 12-mL tubes on an orbital shaker and agitated at 37°C at 300 rpm for 6 hours. The MSC-seeded nanofiber sheets were thoroughly washed with PBS to exclude nonadherent cells.…”

Section: Fabrication Of Nanofiber Sheetsmentioning

confidence: 99%

Development of 3D in vitro platform technology to engineer mesenchymal stem cells

Hosseinkhani¹,

Chen²,

Subramani³

et al. 2012

IJN

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This study aims to develop a three-dimensional in vitro culture system to genetically engineer mesenchymal stem cells (MSC) to express bone morphogenic protein-2. We employed nanofabrication technologies borrowed from the spinning industry, such as electrospinning, to mass-produce identical building blocks in a variety of shapes and sizes to fabricate electrospun nanofiber sheets comprised of composites of poly (glycolic acid) and collagen. Homogenous nanoparticles of cationic biodegradable natural polymer were formed by simple mixing of an aqueous solution of plasmid DNA encoded bone morphogenic protein-2 with the same volume of cationic polysaccharide, dextran-spermine. Rat bone marrow MSC were cultured on electrospun nanofiber sheets comprised of composites of poly (glycolic acid) and collagen prior to the incorporation of the nanoparticles into the nanofiber sheets. Bone morphogenic protein-2 was significantly detected in MSC cultured on nanofiber sheets incorporated with nanoparticles after 2 days compared with MSC cultured on nanofiber sheets incorporated with naked plasmid DNA. We conclude that the incorporation of nanoparticles into nanofiber sheets is a very promising strategy to genetically engineer MSC and can be used for further applications in regenerative medicine therapy.

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Section: Fabrication Of Nanofiber Sheetsmentioning

confidence: 99%

Development of 3D in vitro platform technology to engineer mesenchymal stem cells

Hosseinkhani¹,

Chen²,

Subramani³

et al. 2012

IJN

Self Cite

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“…Hydrogels consist ofa unique class of macromolecular networks that may absorb a large fraction of aqueous solvent. They are particularly suitable for biomedical and tissue engineering applications [5,6] because of their ability to simulate biological tissues [7]. They are used in a variety of applications in bioseparations, agriculture and enhanced oil recovery [8].…”

Section: Introductionmentioning

confidence: 99%

Swelling behavior of poly (2-hydroxyethyl methacrylate) copolymer gels

Sari

Benmouna

Mahlous

et al. 2013

MATEC Web of Conferences

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Abstract. Hydrogels based on 2-hydroxyethyl methacrylate/dimethyl-aminoethyl methacrylate copolymers were prepared by gamma radiation-inducedco-polymerization at low temperature (−78 • C). The swelling behavior of hydrogels was studied by immersion of the polymer discs in buffered solutions at pH from 2 to 10. The hydration process was followed gravimetrically by measuring the water uptake of the discs as a function of time. The results obtained have shown that the swelling behavior is reversible and depends on the polymer nature. Moreover, polymeric discs exhibited a good stability after repeating cycles of hydration in different buffer solutions. Scanning electron microscopy analysis reveals that hydrogel porosity can be controlled.

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“…This synthetic peptide amphiphile promoted osteoblast adhesion, growth, proliferation and bone formation. A synthetic peptide amphiphile with RGD-glutamine-alanine-glycine was synthesized by Hosseinkhani et al, to load the angiogenic growth factor basic fibroblast growth factor (bFGF) for sustained release during tissue regeneration [79]. The same peptide amphiphile system was demonstrated to enhance the proliferation and differentiation of mesenchymal stem cells into an osteogenic lineage [80].…”

Section: Self-assembled Peptide Nanofibres For Tissue Engineeringmentioning

confidence: 99%

“…The hybrid scaffold, when implanted subcutaneously in rat models, was found to form bone homogenously throughout the scaffold [81]. The incorporation of the growth factor bFGF in the hybrid scaffold enhanced the levels of the bone marker osteocalcin and alkaline phosphatase activity, which was not observed in the hybrid scaffolds without bFGF [79]. In a recent work, mucosal cells loaded in a self-assembling peptide scaffold Puramatrix® (RADA16) were successfully employed for middle ear tissue engineering [82].…”

Section: Self-assembled Peptide Nanofibres For Tissue Engineeringmentioning

confidence: 99%

The Integration of Nanotechnology and Biology for Cell Engineering: Promises and Challenges

Krishnan

Sethuraman

2013

Nanomaterials and Nanotechnology

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Introduction: Successful tissue engineering strategies leading to the regeneration of a tissue depend on many factors, starting from the choice of appropriate scaffold material, tailoring the surface functionalities and topography, providing the correct amount of chemical and mechanical stimuli at the appropriate time points, and ensuring the uniform and precise localization of cells. Further challenges arise when more than one cell type has to be employed for the effective regeneration of an organ. Importance: Though the use of nanomaterials has improved tissue engineering, many pitfalls still exist that present a roadblock in the translation of tissue engineering strategies to clinical practice. Apart from employing different materials with distinct surface functionalities and mechanical properties, various strategies have been employed to manipulate the surface topography and chemistry of scaffolds to create a biomimetic microenvironment for effective tissue regeneration. Conclusion: This review provides information about the factors influencing tissue engineering, namely geometry, chemistry, mechanics and cells, and the emerging concepts that may well represent the future of regenerative medicine. Electrospinning techniques and their variants, self-assembly, cell-printing techniques and cell sheet engineering, have all been elaborated in detail. These novel techniques may serve to overcome the challenges currently faced in tissue engineering.

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Enhanced angiogenesis through controlled release of basic fibroblast growth factor from peptide amphiphile for tissue regeneration

Cited by 180 publications

References 29 publications

Development of 3D in vitro platform technology to engineer mesenchymal stem cells

Development of 3D in vitro platform technology to engineer mesenchymal stem cells

Swelling behavior of poly (2-hydroxyethyl methacrylate) copolymer gels

The Integration of Nanotechnology and Biology for Cell Engineering: Promises and Challenges

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