Stimulation of the production of hypericins in in vitro seedlings of Hypericum adenotrichum by some biotic elicitors

Yamaner, Ömer; Erdağ, Bengi; Gökbulut, Cengiz

doi:10.3906/bot-1202-1

Cited by 11 publications

(3 citation statements)

References 33 publications

(40 reference statements)

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“…In the present study, the highest phenolic content was determined in the plant incubated in Suc-45 compared to that of the control. The findings were in agreement with those of Phoenix dactylifera [41], H. adenotrichum [44], and H. sabdariffa [45]. Compatible with our study, an induction of total phenolic compounds has been reported in previous studies after the addition of plant growth regulators [46,47].…”

Section: In Vitro Manipulation Of Antioxidant Capacity and Phenolic C...supporting

confidence: 93%

“…Additionally, administration of BA-1 and NAA-1 alone reduced the amount of all three phenolic acids compared to the control group, thus suppressing the phenolic synthesis. Some stress factors such as UV, drought, excessive water, pathogen infection and carbohydrate (sugar) supplements affect phenolics production in plants [34][35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50]. Plants need different concentration and combination of PGR depending on their genotype for secondary metabolite increase [9].…”

Section: In Vitro Manipulation Of Antioxidant Capacity and Phenolic C...mentioning

confidence: 99%

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The Investigation of the Medicinal Potential of Alcea Kurdica Alef. In Nature and Tissue Culture

Görmez¹

2022

FARMACIA

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Alcea kurdica Alef. (AK) a hollyhock has been used in folk medicine to treat various diseases. Though its extensive utilization in traditional medicine, experimental studies regarding to its phytochemical composition is limited. The aim of this study was to determine the antioxidant capacity and phenolic composition of ethanol extract of AK plant collected from the natural environment in different vegetation periods (June-flowering, July-fruiting, August-seeding) and to control its antioxidant capacity and phenolic composition by in vitro shoot tip culture. AK plant at the flowering period had the highest antioxidant capacity, while the lowest activity was determined at the seeding period according to results. Phenolic acids dominated by gallic, chlorogenic and caffeic acids were determined as the major phenolics. According to in vitro results, the use of 1-naphthylacetic acid (NAA) and 6-benzylaminopurine (BA) alone caused significant decrease in the three phenolic acids content compared to the control. On the other hand, combination of NAA and BA caused significant increase in phenolic acids content compared to the control. Moreover, significant increase in ORAC was observed in AK extract obtained from in vitro Murashige & Skoog (MS) media culture supplemented with NAA and BA combination. 15 min of UV exposure negatively affected the antioxidant capacity and phenolic content. RezumatAlcea kurdica Alef. (AK) a fost folosită în medicina populară pentru a trata diverse boli. Deși este utilizată pe scară largă, studiile experimentale privind compoziția sa fitochimică sunt limitate. Scopul acestui studiu a fost evaluarea caracterului antioxidant și compoziția în polifenoli a extractului etanolic din planta AK colectată din mediul natural în diferite perioade de vegetație (iunie-flori, iulie-fructe, august-semințe), precum și de a modula capacitatea antioxidantă și concentrația polifenolilor prin cultivarea in vitro a vârfurilor de lăstari. Planta AK în perioada de înflorire a avut cea mai mare capacitate antioxidantă, în timp ce cea mai redusă activitate a fost determinată în perioada de însămânțare. Acizii galic, clorogenic și cafeic au fost determinați ca polifenoli principali. Conform rezultatelor in vitro, expunerea la acid 1-naftilacetic (ANA) și la 6benzilaminopurina (BA) a provocat o scădere semnificativă a conținutului celor trei acizi fenolici, în comparație cu martorul. Pe de altă parte, combinația dintre ANA și BA a determinat o creștere semnificativă a conținutului de acizi fenolici în comparație cu martorul. 15 min de expunere la UV au afectat negativ capacitatea antioxidantă și conținutul de fenoli.

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Section: In Vitro Manipulation Of Antioxidant Capacity and Phenolic C...supporting

confidence: 93%

Section: In Vitro Manipulation Of Antioxidant Capacity and Phenolic C...mentioning

confidence: 99%

The Investigation of the Medicinal Potential of Alcea Kurdica Alef. In Nature and Tissue Culture

Görmez¹

2022

FARMACIA

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“…Highest solasodine content during the study was -1 exhibited by S. nigrum 0.179 mg g (9.15 %) at 1% pectin followed , 2015). Enhancement of hypericin biosynthesis by elicitation using pectin has been reported in different Hypericum species (Yamaner et al, 2013;Gadzovska et al, 2015). The present results recommend the beneficial of pectin as an elicitor for S. incanum cell cultures for solasodine production.…”

Section: Resultssupporting

confidence: 70%

Elicitation based enhancement of solasodine production in in-vitro cultures of different Solanum species

Deshmukh¹,

Deshpande²,

Wani³

2023

JEB

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Aim: To study the enhancement of solasodine content using elicitors such as NaCl, pectin, salicylic acid and yeast extract in cell suspension cultures of Solanum incanum, Solanum nigrum, Solanum surattense and Solanum villosum. Methodology: In-vitro callus induction from leaf explants was carried out on MS media supplemented with auxin, 2, 4- Dichlorophenoxyacetic acid (2.0 mg l-1). MS liquid medium supplemented with 2, 4-D (2.0 mg l-1) and varied concentrations of different elicitors were used for cell suspension culture. Results: The elicitor NaCl (150 mM) indicated maximum increment in solasodine production in three Solanum species studied. Salicylic acid with 75 μM resulted in considerable elevation in solasodine content in Solanum spp. Response to elicitation by pectin was high at different concentrations for different species of Solanum. Biotic elicitor yeast extract at 3.0 g l-1 concentration considerably increased solasodine production in S. nigrum and S. villosum. Solanum villosum exhibited best results in terms of solasodine concentration enhancement in response to pectin and yeast extract elicitors whereas Solanum incanum responded best to pectin followed by NaCl. Interpretation: The current results indicated that NaCl, pectin, salicylic acid and yeast extract can be effectively applied as influential elicitors for the enhancing the production of solasodine in cell suspension culture of different Solanum species. Key words: Callus, Cell suspension culture, Elicitors, Solanum, Solasodine, Steroidal alkaloids

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Secondary Metabolites in Shoot Cultures of Hypericum

Coste¹,

Pop²,

Halmágyi³

et al. 2019

Reference Series in Phytochemistry

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Stimulation of the production of hypericins in in vitro seedlings of Hypericum adenotrichum by some biotic elicitors

Cited by 11 publications

References 33 publications

The Investigation of the Medicinal Potential of Alcea Kurdica Alef. In Nature and Tissue Culture

The Investigation of the Medicinal Potential of Alcea Kurdica Alef. In Nature and Tissue Culture

Elicitation based enhancement of solasodine production in in-vitro cultures of different Solanum species

Secondary Metabolites in Shoot Cultures of Hypericum

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