Stimulation of the nitric oxide/cyclic guanosine monophosphate signaling pathway elicits human sperm chemotaxis in vitro

Miraglia, Erica; Rullo, Maria Luisa; Bosìa, Amalia; Massobrio, M; Revelli, Alberto; Ghigo, Dario

doi:10.1016/j.fertnstert.2006.07.1540

Cited by 41 publications

(46 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Rp-8-Br-cGMPS, a PRKG1 inhibitor (Kawada et al 1997), abolished the positive effect exerted by both GSNO and 8-Br-cGMP on sperm motility, suggesting that the effect of endogenous or exogenous cGMP on sperm movement is mediated by PRKG1 activity. Thus, from our data, PRKG1 seems to play a role in mediating not only the NO-elicited chemotaxis and the acrosome reaction (Revelli et al 2001, Miraglia et al 2007), but also in modulating several sperm motion patterns. On the other hand, we did not observe any effect of the cGMP/ PRKG1 pathway modulators on the onset of hyperactivated sperm motility.…”

Section: Discussionmentioning

confidence: 59%

“…In a previous work, we have demonstrated that GSNO and 8-Br-cGMP exerted a significant chemotactic effect on human spermatozoa without affecting their motion parameters (Miraglia et al 2007). In that study, both substances were used at different concentrations and time periods compared with those used in this investigation.…”

Section: Discussionmentioning

confidence: 95%

“…On the other hand, 8-Br-cGMP was used in the previous study at a 1 mM concentration, twofold higher than the one used in the present work. Moreover, in our previous work, we investigated the sperm motion parameters only after 20 min of incubation with GSNO and 8-Br-cGMP, whereas in the present work we used longer (30-90 min) time periods of observation (Miraglia et al 2007). Since the intracellular levels of cGMP measured after incubation with either GSNO or 8-Br-cGMP were respectively similar in both the experimental works, in spite of the different incubation times and concentrations used, it is likely that these compounds exert a significant effect on sperm motility only when the level of intracellular cGMP is maintained increased for a time longer than the one necessary for cGMP to modulate chemotaxis.…”

Section: Discussionmentioning

confidence: 99%

“…Three NOS isoforms have been described: endothelial (eNOS, NOS3), neuronal (nNOS, NOS1), and inducible (iNOS or NOS2; Nathan & Xie 1994). NO has been demonstrated to play a role in a variety of functions in the human reproductive tract, including sperm motility (Lewis et al 1996), chemotaxis (Miraglia et al 2007), and sperm-zona pellucida binding ability (Sengoku et al 1998). NOS isoforms have been localized in the acrosome and tail of human, mouse, and bovine spermatozoa (Herrero et al 1996, Meiser & Schulz 2003, and low motility spermatozoa have been shown to exhibit aberrant patterns of NOS3 immunostaining (O'Bryan et al 1998).…”

Section: Introductionmentioning

confidence: 99%

“…The NO donors sodium nitroprusside and SPNO have been shown to increase the intracellular levels of cGMP in human (Zhang & Zheng 1996, Revelli et al 2001 and murine (Herrero et al 1998) spermatozoa respectively, and recently the sGC has been identified in human sperm by immunoblotting (Willipinski-Stapelfeldt et al 2004). Although its levels in human sperm are about 100-fold lower than the cAMP content (Willipinski-Stapelfeldt et al 2004), cGMP has been implicated in several sperm signaling pathways functions, such as capacitation, acrosome reaction, chemotaxis, and sperm-egg interaction (Revelli et al 2001, 2002, Herrero et al 2003, Miraglia et al 2007. cGMP is thought to modulate also sperm motility.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Nitric oxide stimulates human sperm motility via activation of the cyclic GMP/protein kinase G signaling pathway

Miraglia¹,

Angelis²,

Gazzano³

et al. 2011

Reproduction

Self Cite

View full text Add to dashboard Cite

Nitric oxide (NO), a modulator of several physiological processes, is involved in different human sperm functions. We have investigated whether NO may stimulate the motility of human spermatozoa via activation of the soluble guanylate cyclase (sGC)/cGMP pathway. Sperm samples obtained by masturbation from 70 normozoospermic patients were processed by the swim-up technique. The kinetic parameters of the motile sperm-rich fractions were assessed by computer-assisted sperm analysis. After a 30-90 min incubation, the NO donor S-nitrosoglutathione (GSNO) exerted a significant enhancing effect on progressive motility (77, 78, and 78% vs 66, 65, and 62% of the control at the corresponding time), straight linear velocity (44, 49, and 48 mm/s vs 34, 35, and 35.5 mm/s), curvilinear velocity (81, 83, and 84 mm/s vs 68 mm/s), and average path velocity (52, 57, and 54 mm/s vs 40, 42, and 42 mm/s) at 5 mM but not at lower concentrations, and in parallel increased the synthesis of cGMP. A similar effect was obtained with the NO donor spermine NONOate after 30 and 60 min. The GSNO-induced effects on sperm motility were abolished by 1H-[1,2,4]oxadiazolo-[4,3-a]quinoxalin-1-one (a specific sGC inhibitor) and mimicked by 8-bromo-cGMP (8-Br-cGMP; a cell-permeating cGMP analog); the treatment with Rp-8-Br-cGMPS (an inhibitor of cGMP-dependent protein kinases) prevented both the GSNO-and the 8-Br-cGMP-induced responses. On the contrary, we did not observe any effect of the cGMP/PRKG1 (PKG) pathway modulators on the onset of hyperactivated sperm motility. Our results suggest that NO stimulates human sperm motility via the activation of sGC, the subsequent synthesis of cGMP, and the activation of cGMP-dependent protein kinases.

show abstract

Section: Discussionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Nitric oxide stimulates human sperm motility via activation of the cyclic GMP/protein kinase G signaling pathway

Miraglia¹,

Angelis²,

Gazzano³

et al. 2011

Reproduction

Self Cite

View full text Add to dashboard Cite

show abstract

Calcium, NO, and cGMP Signaling in Plant Cell Polarity

Prado

Feijó

2009

Nitric Oxide in Plant Physiology

View full text Add to dashboard Cite

Reactive oxygen, nitrogen, and sulfur species in human male fertility. A crossroad of cellular signaling and pathology

et al. 2019

View full text Add to dashboard Cite

Infertility is a significant global health problem that currently affects one of six couples in reproductive age. The quality of male reproductive cells dramatically decreased over the last years and almost every aspect of modern life additionally worsen sperm functional parameters that consequently markedly increase male infertility. This clearly points out the importance of finding a new approach to treat male infertility. Redox signaling mediated by reactive oxygen, nitrogen and sulfur species (ROS, RNS, and RSS respectively), has appeared important for sperm reproductive function. Present review summarizes the current knowledge of ROS, RNS, and RSS in male reproductive biology and identifies potential targets for development of novel pharmacological and therapeutic approaches for male infertility by targeted therapeutic modulation of redox signaling.

show abstract

Stimulation of the nitric oxide/cyclic guanosine monophosphate signaling pathway elicits human sperm chemotaxis in vitro

Cited by 41 publications

References 24 publications

Nitric oxide stimulates human sperm motility via activation of the cyclic GMP/protein kinase G signaling pathway

Nitric oxide stimulates human sperm motility via activation of the cyclic GMP/protein kinase G signaling pathway

Calcium, NO, and cGMP Signaling in Plant Cell Polarity

Reactive oxygen, nitrogen, and sulfur species in human male fertility. A crossroad of cellular signaling and pathology

Contact Info

Product

Resources

About