Reactive oxygen, nitrogen, and sulfur species in human male fertility. A crossroad of cellular signaling and pathology

Otašević, Vesna; Stančić, Ana; Korać, Aleksandra; Janković, Aleksandra; Korać, Bato

doi:10.1002/biof.1535

Cited by 24 publications

(21 citation statements)

References 139 publications

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“…Indeed, when superoxide anion generation exceeds the limited antioxidant capacity of the sperm cell, it has the ability to propagate the formation of non-radicals, including hydrogen peroxide (H 2 O 2 ), and in the presence of Fe 3+ , the formation of alternative radicals such the hydroxyl anion (OH − ) via Haber-Weiss and Fenton reactions [26]. Additional ROS containing nitrogen atoms (such as NO, NO 3 − , NO − , N 2 O, and HNO 3 ) are also able to be formed [27,28] and collectively, these powerful oxidants have the potential to trigger the peroxidation of membrane lipids and a concomitant loss of sperm function [29]. In studies conducted in rats it has been shown that the presence of dead spermatozoa can also promote higher than normal levels of H 2 O 2 [30].…”

Section: Spermatozoamentioning

confidence: 99%

Molecular Changes Induced by Oxidative Stress that Impair Human Sperm Motility

2020

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A state of oxidative stress (OS) and the presence of reactive oxygen species (ROS) in the male reproductive tract are strongly correlated with infertility. While physiological levels of ROS are necessary for normal sperm functioning, elevated ROS production can overwhelm the cell’s limited antioxidant defenses leading to dysfunction and loss of fertilizing potential. Among the deleterious pleiotropic impacts arising from OS, sperm motility appears to be particularly vulnerable. Here, we present a mechanistic account for how OS contributes to altered sperm motility profiles. In our model, it is suggested that the abundant polyunsaturated fatty acids (PUFAs) residing in the sperm membrane serve to sensitize the male germ cell to ROS attack by virtue of their ability to act as substrates for lipid peroxidation (LPO) cascades. Upon initiation, LPO leads to dramatic remodeling of the composition and biophysical properties of sperm membranes and, in the case of the mitochondria, this manifests in a dissipation of membrane potential, electron leakage, increased ROS production and reduced capacity for energy production. This situation is exacerbated by the production of cytotoxic LPO byproducts such as 4-hydroxynonenal, which dysregulate molecules associated with sperm bioenergetic pathways as well as the structural and signaling components of the motility apparatus. The impact of ROS also extends to lesions in the paternal genome, as is commonly seen in the defective spermatozoa of asthenozoospermic males. Concluding, the presence of OS in the male reproductive tract is strongly and positively correlated with reduced sperm motility and fertilizing potential, thus providing a rational target for the development of new therapeutic interventions.

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Section: Spermatozoamentioning

confidence: 99%

Molecular Changes Induced by Oxidative Stress that Impair Human Sperm Motility

2020

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“…Substantial information is available regarding the role of NO in crucial sperm processes prior to fertilization, such as capacitation, hyperactivation, acrosome reaction, and zona pellucida binding [19,[40][41][42]. Furthermore, the role of NO has been widely investigated during semen handling and storage [43,44].…”

Section: Mechanisms Of Action Of No In Spermatozoamentioning

confidence: 99%

“…A third mechanism of action occurs at high concentrations of NO, which directly provokes a post-translational modification of proteins, reversibly by S-nitrosylation or irreversibly by tyrosine nitration [40]. Within the human spermatozoa, more than 200 proteins have been identified that are modified by NO via the process of S-nitrosylation [58], which is the covalent union of NO and sulfur of cysteine, forming a nitrosothiol group (-SNO) within the molecule.…”

Section: Mechanisms Of Action Of No In Spermatozoamentioning

confidence: 99%

“…As mitochondria are the main source of ROS within the spermatozoon, as well as a major source of energy for movement, the decrease in sperm motility in response to oxidative stress may be linked to alterations of mitochondrial activity [80]. [40]. Ultimately, excessive RNS can be responsible for lipid, protein, and DNA impairment [81].…”

Section: The Role Of No and H 2 S In Oxidative Stressmentioning

confidence: 99%

“…Mishanina et al [72] list a wide range of biologically active RSS with H 2 S as a common precursor. Like RNS and ROS, the concentration of RSS is crucial for physiological activity, as in supraphysiological concentrations, RSS exert a negative effect on sperm cells [40]. In a study by Wang et al [39], asthenozoospermic men showed decreased H 2 S concentrations in seminal plasma, and the application of a H 2 S donor (GYY4137) improved the total and progressive sperm motility.…”

Section: H 2 S and Reactive Sulfur Speciesmentioning

confidence: 99%

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The Roles of NO and H2S in Sperm Biology: Recent Advances and New Perspectives

Kadlec

Ros-Santaella

Pintus

2020

IJMS

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After being historically considered as noxious agents, nitric oxide (NO) and hydrogen sulfide (H2S) are now listed as gasotransmitters, gaseous molecules that play a key role in a variety of cellular functions. Both NO and H2S are endogenously produced, enzymatically or non-enzymatically, and interact with each other in a range of cells and tissues. In spite of the great advances achieved in recent decades in other biological systems, knowledge about H2S function and interactions with NO in sperm biology is in its infancy. Here, we aim to provide an update on the importance of these molecules in the physiology of the male gamete. Special emphasis is given to the most recent advances in the metabolism, mechanisms of action, and effects (both physiological and pathophysiological) of these gasotransmitters. This manuscript also illustrates the physiological implications of NO and H2S observed in other cell types, which might be important for sperm function. The relevance of these gasotransmitters to several signaling pathways within sperm cells highlights their potential use for the improvement and successful application of assisted reproductive technologies.

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The protective effect of aqueous extract of Stevia rebaudiana against tartrazine toxicity in male Wistar rat

Hanna,

Beshay,

El. Shafee

et al. 2023

Cell Biochemistry & Function

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Tartrazine is a yellow colouring agent that is commonly used in foods; however, high dosages of Tartrazine affect fertility and create oxidative stress by generating free radicals. A plant species known as Stevia rebaudiana has natural antioxidants that show promise for protecting testicular tissue. Consequently, this study was intended to examine the ameliorative effect of the aqueous extract of S. rebaudiana (Stevia) on the fertility of male Wistar rats induced by the daily oral intake of Tartrazine. Utilizing gas chromatography–mass spectrometry, phytochemical identification was accomplished for Stevia extract. Study groups were separated into several groups: the first group (the control) got distilled water for up to 56 days; the Stevia group (1000 mg/kg), the Tartrazine group (300 mg/kg) and the Stevia and Tartrazine group (the group was given Tartrazine after 1 h of Stevia extract intake). Also, the oxidative damage in testicular tissues was assessed by measuring the levels of malondialdehyde (MDA) and antioxidants (catalase [CAT], superoxide dismutase [SOD] and glutathione reductase [GSH]). Further, histological alterations were examined. In addition, cyclic AMP‐responsive element modulator (Crem) gene expression levels and their relative proteins were measured in the testicular tissues using quantitative real‐time polymerase chain reaction and enzyme‐linked immunosorbent assays, respectively. Sperm analysis and testosterone concentration were also performed. SPSS version 25 was used for the analysis of results while (p < .05) was regarded as significant. Compared with the control group, the results demonstrated that Tartrazine caused a significant reduction (p < .05) in the testosterone hormone level (0.70 ± 0.21) and the Crem protein quantity (1.21 ± 0.23) in the treated Tartrazine group. Also, it had a significant decrease (p < .05) in sperm motility, viability, count and antioxidant levels. Moreover, there was a significant increase (p < .05) in sperm abnormalities, MDA level (7.40 ± 1.10), kidney and liver function parameters, and DNA degradation in the treated Tartrazine group compared with the control group. On the contrary, the Stevia extract intake enhanced the testosterone (2.50 ± 0.60), antioxidants and Crem protein levels (2.33 ± 0.10) with an improvement in sperm quality in the Stevia and Tartrazine‐treated group compared with the Tartrazine group. Stevia also caused a significant decrease (p < .05) in the MDA level (3.20 ± 0.20), and sperm abnormalities with an enhancement of the liver and kidney function parameters in the Stevia and Tartrazine‐treated group compared to the Tartrazine group. Stevia administration has a protective effect on the testicular tissues and sperm quality against toxicity induced by Tartrazine exposure, so it will be a good antioxidant drug to be administered daily before daily administration of Tartrazine.

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Reactive oxygen, nitrogen, and sulfur species in human male fertility. A crossroad of cellular signaling and pathology

Cited by 24 publications

References 139 publications

Molecular Changes Induced by Oxidative Stress that Impair Human Sperm Motility

Molecular Changes Induced by Oxidative Stress that Impair Human Sperm Motility

The Roles of NO and H2S in Sperm Biology: Recent Advances and New Perspectives

The protective effect of aqueous extract of Stevia rebaudiana against tartrazine toxicity in male Wistar rat

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