Stimulation of Spermatogenesis and Synthesis of Testosterone by Allotransplantation of Neonatal Testicular Tissue under Tunica Albuginea of Cryptorchid Testis

Кирпатовский, В. И.; Ефремов, Г. Д.; Frolova, Elena Vladimirovna; Kudryavtseva, L V

doi:10.1007/s10517-019-04381-8

Cited by 4 publications

(2 citation statements)

References 12 publications

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“…Пересаженная тестикулярная ткань не только сохраняла жизнеспособность в течение 6 месяцев, но и происходило развитие незрелой ткани с формированием полноценных семенных канальцев, а также зачатков придатка яичка и семявыносящего протока, что свидетельствовало о паракринной стимуляции органогенеза [20]. При трансплантации под белочную оболочку крипторхированного яичка пересаженная ткань неонатального тестикула стимулировала регенерацию семенных канальцев с восстановлением нарушенного сперматогенеза и активировала нарушенный синтез тестостерона [21].…”

Section: Introductionunclassified

Comparative characteristics of the effectiveness of preventing the progression of CRF using therapy with secretome of embryonic cell and transplantation of neonatal tissues.

V.I.,

A.V.,

S.A.

et al. 2024

ECU

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Introduction. Literature data and our own research indicate that therapy with immature cell secretion products (secretome) has a pronounced nephroprotective effect both in acute kidney injury and in chronic renal failure (CRF). Considering that the source of the complex of signaling molecules stimulating reparative processes in organs are low-differentiated cells of functionally and anatomically immature organs in a state of deveopment, the question arises to what extent tissue transplantation of these organs reproduces the effect of therapy with a secretome of stem cells in relation to the progression of CRF. Material and methods. The experiments were carried out on 40 outbred male rats weighing 260-290 g, in which CRF was modeled by resection of 4/5 functionally active renal parenchyma. In the 1st series (control), no therapeutic actions were performed. In the 2nd series, after modeling CRF, a course of therapy with Cellex, the active component of which is the secretome of pig embryonic brain cells, was carried out in the form of 2 10-day courses with a 10-day break between them. In the 3rd and 4th series, immediately after CRF modeling, kidney or testicle tissue obtained from newborn baby rats (1-2 days after birth) was injected under the capsule of the resected kidney as a source of immature cells. Tissues of different organs were used to assess the severity of the tissue-specific effect of therapy. Results. 7 days after the modeling of CRF in all series, a deterioration of more than 2 times of all functional parameters was revealed. After 1 month, in the control and in the 2nd and 3rd series, a tendency to an increase in GFR was revealed, which did not reach statistical significance, whereas in the 4th series, the increase in GFR turned out to be statistically significant. After 2 months in the control series, there was a repeated decrease in the GFR index, whereas in all experimental series, GFR continued to increase or remained at subnormal values. The activity of tubular reabsorption of sodium and calcium after a significant decrease after 7 days to 1 month in the control series increased slightly (but unreliably), and after 2 months they again decreased to values 2 times lower than normal. In all experimental series, after 1 and 2 months, the reabsorption of these electrolytes was at values close to normal. The improvement was especially pronounced in groups 2 and 4. After 7 days, the activity of intracellular enzymes (aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH)) in the blood increased in all series with a gradual decrease in hyperfermentemia after 2 months, more pronounced in group 4, as well as in all experimental groups with respect to LDH. Histological examination of the condition of neonatal transplants revealed the destruction of transplanted kidney tissue and a viable testicular graft. Conclusion. Transplantation of neonatal organ tissue under a kidney capsule to rats with CRF has a comparable nephroprotective effect with therapy with the secretome of embryonic cells (Cellex drug). A more pronounced improvement in functional parameters during testicular tissue transplantation is explained by the preservation of the viability of the graft.

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Section: Introductionunclassified

Comparative characteristics of the effectiveness of preventing the progression of CRF using therapy with secretome of embryonic cell and transplantation of neonatal tissues.

V.I.,

A.V.,

S.A.

et al. 2024

ECU

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“…They are critical for the mammal reproductive function because of their production of sex hormones. All these make ICs very important material for transplantation [3,4].…”

Section: Introductionmentioning

confidence: 99%

The impact of cryopreservation with polyvinyl alcohol on the survival and functional activity of rat testis interstitial cells

Pakhomov

Sidorenko

2021

Biopolym. Cell

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Maternal exposure to PM2.5 induces the testicular cell apoptosis in offspring triggered by the UPR-mediated JNK pathway

Ren

Jiang

Huang

et al. 2022

Toxicology Research

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Contemporary exposure to PM2.5 has been reported to disrupt spermatogenesis. However, the subsequent toxicological responses and the mechanisms of male reproductive damage in offspring induced by maternal exposure to PM2.5 remain largely unknown. For the first time, this study aimed to explore the apoptotic response in spermatogenesis of male offspring following maternal exposure to PM2.5 and its mechanisms. The C57BL/6 mice with vaginal plugs were randomly divided into four groups. Mice in the PM2.5 groups were intratracheally exposed to PM2.5 (4.8 mg/kg body weight, 43.2 mg/kg body weight) during pregnancy (every 3 days, six times in total). The mice in the membrane control group were treated similarly to the PM2.5 groups, applying only PM2.5 sampling membrane, while mice in the control group were kept untreated. The results showed that maternal exposure to PM2.5 during pregnancy resulted in structural lesions of the testis, reduced numbers of primary spermatocytes and spermatids, decreased sperm count and quality, shortened diameter of seminiferous tubules, and reduced testosterone and ABP in the offspring testes. Furthermore, cell apoptosis was increased and protein expression of IRE-1/P-JNK/cleaved caspase-12/cleaved caspase-3 was activated. These findings suggested that maternal exposure to PM2.5 may affect spermatogenesis by increasing apoptosis through activation of UPR-mediated JNK apoptotic pathway in offspring testicles and by reducing testosterone secretion.

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Stimulation of Spermatogenesis and Synthesis of Testosterone by Allotransplantation of Neonatal Testicular Tissue under Tunica Albuginea of Cryptorchid Testis

Cited by 4 publications

References 12 publications

Comparative characteristics of the effectiveness of preventing the progression of CRF using therapy with secretome of embryonic cell and transplantation of neonatal tissues.

Comparative characteristics of the effectiveness of preventing the progression of CRF using therapy with secretome of embryonic cell and transplantation of neonatal tissues.

The impact of cryopreservation with polyvinyl alcohol on the survival and functional activity of rat testis interstitial cells

Maternal exposure to PM2.5 induces the testicular cell apoptosis in offspring triggered by the UPR-mediated JNK pathway

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