Stimulation of murine hemopoietic colony formation by human IL-6.

Abstract

A novel hemopoietic CSF has been identified in the medium conditioned by lectin-stimulated human T cells. The cDNA clone encoding this factor, isolated by functional expression cloning in monkey cos-1 cells, proved to be identical with the cDNA encoding the cytokine B cell stimulatory factor-2/IFN-beta 2, a factor now known as IL-6. In the murine system, IL-6 indirectly supports the formation of several different types of hemopoietic colonies, including those derived from early blast cells, and directly supports the proliferation of granulocyte/macrophage progenitors. These results expand the range of known target cells of IL-6 to include hemopoietic progenitors in addition to B cells, T cells, and fibroblasts and provide further evidence that this cytokine plays an important role within a network of interacting cytokines that regulates many different biologic responses.

1.9 Acrystal structure of interleukin 6: implications for a novel mode of receptor dimerization and signaling

“…Interleukin 6 (IL‐6) is a pleiotropic cytokine with a variety of stimulatory effects on hematopoietic cells and cells of the immune system (Hirano et al ., 1986; Wong et al ., 1988; Kishimoto et al ., 1992). Major cellular targets include B lymphocytes, T lymphocytes, the enhancement of hematopoietic colony formation and the production of acute phase response proteins in the liver (Mackiewicz et al ., 1992).…”

“…also the LIF, CNTF, oncostatin M and the IL-11 receptors (Taga et al, 1992); therefore, the α chain distribution Interleukin 6 (IL-6) is a pleiotropic cytokine with a variety dictates the cellular response (Kishimoto et al, 1992). of stimulatory effects on hematopoietic cells and cells of Signal transduction by IL-6 follows the dimerization the immune system (Hirano et al, 1986;Wong et al, of gp130, which activates a bound JAK2 (Argetsinger 1988;Kishimoto et al, 1992). Major cellular targets et al, 1993).…”