Effect of Timing of Lymphokine Presentation on Generation of Cytotoxic T Lymphocytes

Stringfellow, Monica M.; Wilson, Richard E.; Burakoff, Steven J.; Herrmann, Steven H.

doi:10.1001/archsurg.1989.01410010091019

Cited by 2 publications

(1 citation statement)

References 18 publications

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“…increased levels of lL-6 have been observed in homogenized biopsies from IBD patients [25], lL-6 has been shown lo provide an accessory funclion in T cell and B eeli activation and proliferation [21] and thereby IL-6 augments cellular immune events induced during mucosal inflammation. IL-6 stimulates lhe cytotoxic T cell response [51] and enhances IL-2 receptor expression on T lymphocytes [52]. IL-6 participates in promoting the differentiation of B iymphocyles into plasma cells [53] and enhances immunoglobulin synthesis by LPMNC from lhe human intestine [54].…”

Section: Discussionmentioning

confidence: 99%

Enhand secretion of tumour necrosis factor-alpha, IL-6, and IL-1β by isolated lamina ropria monouclear cells from patients with ulcretive cilitis and Crohn's disease

Reinecker

Steffen

Witthoeft

et al. 1993

Clinical and Experimental Immunology

805

343

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The perpetuation of inflammation in ulcerative colitis and Crohn's disease may be regulated in part by an increased secretion of proinflammatory cytokines due to either an appropriate response to initial stimulating agents, and/or due to an impaired down-regulation of cytokine secretion. The aim of this study was to determine the secretion patterns of the proinflammatory cytokines tumour necrosis factor-alpha (TNF-alpha), IL-6 and IL-1 beta, from isolated lamina propria mononuclear cells (LPMNC) isolated from colonic biopsies from patients with untreated ulcerative colitis or Crohn's disease. LPMNC isolated from involved inflammatory bowel disease (IBD) mucosa spontaneously produced increased amounts of TNF-alpha, and IL-6, and IL-1 beta. The TNF-alpha secretion from IBD LPMNC could be further enhanced by pokeweed mitogen stimulation. The secretion patterns of TNF-alpha and IL-1 beta by LPMNC from patients with either ulcerative colitis or Crohn's disease demonstrated a close correlation with the degree of tissue involvement and mucosal inflammation. LPMNC from non-involved ulcerative colitis mucosa secreted markedly increased levels of IL-6 compared with non-involved Crohn's disease mucosa or control mucosa. The heightened IL-6 secretion from LPMNC from non-involved ulcerative colitis mucosa without visible or microscopic signs of inflammation indicates that the pathophysiologic mechanisms involved in the initiation of inflammation may differ between ulcerative colitis and Crohn's disease. The determination of proinflammatory cytokine secretion by isolated LPMNC from colonoscopic biopsies may be a sensitive method for monitoring the severity of mucosal inflammation in IBD patients.

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