Enhand secretion of tumour necrosis factor-alpha, IL-6, and IL-1β by isolated lamina ropria monouclear cells from patients with ulcretive cilitis and Crohn's disease

Reinecker, Hans–Christian; Steffen, Martín; Witthoeft, T.; Pflueger, I.; Schreiber, Stefan; MacDermott, Richard P.; Raedler, Andreas

doi:10.1111/j.1365-2249.1993.tb05997.x

Cited by 805 publications

(380 citation statements)

References 46 publications

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“…For example, enhanced capacity to produce IL-1/3 by circulating mononuclear cells in patients with active UC and CD has been found [27]. Mucosal mononuclear cells isolated from colonic biopsies of IBD patients with active inflammation produced more IL-1/3 compared with those isolated from normal intestine [17,28]. The concentration of IL-1/3 mRNA was found to be increased in intestinal mucosa of IBD patients and correlated with disease activity [29].…”

Section: Discussionmentioning

confidence: 99%

Allelic polymorphism in IL-1β and IL-1 receptor antagonist (IL-1Ra) genes in inflammatory bowel disease

Bioque

Crusius

Koutroubakis

et al. 1995

Clinical and Experimental Immunology

185

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SUMMARY Recent reports have shown that allele 2 of the IL‐1 receptor antagonist (IL‐1Ra) gene is over‐represented in ulcerative colitis (UC). Healthy individuals carrying allele 2 of this gene have increased production of IL‐1Ra protein. Since the final outcome of the biological effects of IL‐1β may depend on the relative proportion of these two cytokines, we have studied if a TaqI polymorphism in the IL‐1β gene, which is relevant to IL‐1β protein production, may be involved in the genetic susceptibility to UC and Crohn's disease (CD), in association with the established IL‐1Ra gene polymorphism. Polymorphisms in the closely linked genes for IL‐1β and IL‐1Ra were typed in 100 unrelated Dutch patients with UC, 79 with CD, and 71 healthy controls. The polymorphic regions in exon 5 of the IL‐1β gene and in intron 2 of the IL‐1Ra gene, were studied by polymerase chain reaction (PCR)‐based methods. The IL‐1β allele frequencies in UC and CD patients did not differ from those in healthy controls. In order to study if the IL‐1β gene polymorphism might participate synergistically with the IL‐1Ra gene polymorphism in susceptibility to UC and CD, individuals were distributed into carriers and non‐carriers of allele 2 of the genes encoding IL‐1β and IL‐1Ra, in each of the patient groups and controls. Results indicated a significant association of this pair of genes, estimated by the odds ratio (OR) after performing Fisher's exact test, in the UC group (P= 0·023, OR = 2·81), as well as in the CD group (P= 0·01, OR = 3·79). Thus, non‐carriers of IL‐1β allele 2 were more often present in the subgroup of patients carrying the IL‐1Ra allele 2. By contrast, no association of these alleles was detected in the group of healthy controls (P= 1·00, OR = 0·92). These results suggest that the IL‐1β/IL‐1Ra allelic cluster may participate in defining the biological basis of predisposition to chronic inflammatory bowel diseases.

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Section: Discussionmentioning

confidence: 99%

Allelic polymorphism in IL-1β and IL-1 receptor antagonist (IL-1Ra) genes in inflammatory bowel disease

Bioque

Crusius

Koutroubakis

et al. 1995

Clinical and Experimental Immunology

185

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“…70 In addition, lamina propria T cells from IBD patients secrete elevated levels of IL-6, and IL-6/STAT-3 signaling is upregulated. 71,72 However, there are differences between the two clinical forms of IBD, with lamina propria T cells from CD patients showing Th1 polarization, whereas those of UC patients express Th2 cytokines. 73 In addition, there is enhanced production of IL-10 in colonic tissue of UC patients.…”

Section: Probiotics In Infl Ammatory Diseases Of the Bowelmentioning

confidence: 99%

Probiotics and immunity

et al. 2009

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Probiotics are defi ned as live microorganisms that, when administered in adequate amounts, confer a health benefi t on the host, including the gastrointestinal tract. While this benefi cial effect was originally thought to stem from improvements in the intestinal microbial balance, there is now substantial evidence that probiotics can also provide benefi ts by modulating immune functions. In animal models, probiotic supplementation is able to provide protection from spontaneous and chemically induced colitis by downregulating infl ammatory cytokines or inducing regulatory mechanisms in a strain-specifi c manner. In animal models of allergen sensitization and murine models of asthma and allergic rhinitis, orally administered probiotics can straindependently decrease allergen-specifi c IgE production, in part by modulating systemic cytokine production. Certain probiotics have been shown to decrease airway hyperresponsiveness and infl ammation by inducing regulatory mechanisms. Promising results have been obtained with probiotics in the treatment of human infl ammatory diseases of the intestine and in the prevention and treatment of atopic eczema in neonates and infants. However, the fi ndings are too variable to allow fi rm conclusions as to the effectiveness of specifi c probiotics in these conditions.

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“…LPMNCs were isolated from intestinal mucosal biopsy samples as described previously. 11,12 These cells were 96-98% mononuclear, the percentage of macrophages was controlled to be stable by both nonspecific esterase detection (Sigma, St. Louis, MO, USA) and staining for CD68 (Dako Diagnostica GmbH, Hamburg, Germany) using the alkaline phosphatase antialkaline phosphatase technique (Vector Laboratories, Burlingame, CA, USA; Dako, Carpinteria, CA, USA). Additional controls for the representation of different cell populations (CD20 (10-30%), CD3 (32-64%), CD4 (25-33%), CD8 (12-17%) were performed by flow cytometry (data not shown) (antibodies were purchased from Becton Dickinson, Mountainview, CA, USA).…”

Section: Human Lamina Propria Mononuclear Cellsmentioning

confidence: 99%

“…The distribution of subpopulations in the LPMNC preparations was found to be similar to that in earlier flow cytometry studies. [11][12][13] Contamination of epithelial cells was seen as less than 0.5% by both morphology and cytokeratin antibody (Dako) staining. 14 …”

Section: Human Lamina Propria Mononuclear Cellsmentioning

confidence: 99%

Systematic expression profiling of innate immune genes defines a complex pattern of immunosenescence in peripheral and intestinal leukocytes

et al. 2008

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Immunosenescence is characterized by a quantitative decline of adequate immune responses, which renders the elderly individual particularly susceptible to bacterial, viral and fungal pathogens. Whereas changes of the aging adaptive immune system (for example, reduced immunoglobulin secretion) have been extensively characterized, alterations of the innate immune system are still poorly understood. The aim of the present study was to systematically examine mRNA expression levels of innate immune genes and proinflammatory cytokines in peripheral and intestinal leukocytes of subjects of different ages. In both, whole blood samples and in colonic biopsies most of the Toll-like receptors (TLRs) and nucleotide-binding and oligomerization domain-like receptors (NLRs) transcript levels were significantly downregulated in elderly subjects (90-99 years). Older individuals, when compared to the younger, exhibited an increased expression and/or secretion of proinflammatory cytokines by peripheral and intestinal leukocytes as well as an increased level of nuclear factor-kB activation in colonic biopsies. The observed downregulation of TLRs and NLRs during the aging process may contribute to the lack of effective recognition of invading pathogens or the commensal flora. This effect results in aberrant secondary immune cell activation and could significantly contribute to morbidity and mortality at advanced age.

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Enhand secretion of tumour necrosis factor-alpha, IL-6, and IL-1β by isolated lamina ropria monouclear cells from patients with ulcretive cilitis and Crohn's disease

Cited by 805 publications

References 46 publications

Allelic polymorphism in IL-1β and IL-1 receptor antagonist (IL-1Ra) genes in inflammatory bowel disease

Allelic polymorphism in IL-1β and IL-1 receptor antagonist (IL-1Ra) genes in inflammatory bowel disease

Probiotics and immunity

Systematic expression profiling of innate immune genes defines a complex pattern of immunosenescence in peripheral and intestinal leukocytes

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