Stimulation of ligninolytic enzyme production in Phanerochaete chrysosporium by polyoxyalkanes

Abstract

I . G R G I C A N D A . P E R D I H . 2003.Aims: Poly(ethylene glycol) (PEG) and some substances similar to PEG in chemical structure were tested as stimulators of ligninolytic enzyme production in shaken culture of Phanerochaete chrysosporium. Methods and Results: The substances that caused high enzymatic activity were linear polymers [poly(ethylene glycol), poly(propylene glycol), poly(butylene glycol) and poly(vinyl alcohol)] and cyclic polymers (crown ether). They can have terminal groups other than -OH [PEG (di)methyl ether, PEG sulphate, PEG derivative with the amino group and xanthate]. The maximum lignin peroxidase activities were compared with the surface pressure caused by the stimulator. Addition of polymers composed of charged monomer units did not increase the enzymatic activity and the fungi did not grow at all on addition of polymers having a fixed positive charge. Conclusions: Lignin peroxidase activity was increased after the addition of polymers with uncharged monomer units. It was higher and its maximum was reached in a shorter time on addition of polymers with higher molecular weights. Significance and Impact of Study: Beside Tweens there are several polymers that stimulate ligninolytic enzyme production in shaken culture of P. chrysosporium. Their characteristics are: similarity to PEG in chemical structure, having uncharged monomer units and high molecular weight.

Effects of Pluronic F68 on Manganese Peroxidase Production by Pelletized Phanerochaete chrysosporium

“…Ten percent of the additive solutions were autoclaved and added at the beginning of fermentation. The concentrations of Pluronic F68, Tween 80, PEG 8000, and BSA were set at 0.1%, 0.05%, 0.1%, and 0.04%, respectively, according to literatures [17,18]. The cultivations were carried out at different medium sizes with three replicates (50 mL in 125-mL Erlenmeyer flask, 100 mL in 250-mL Erlenmeyer flask, and 200 mL in 500-mL Erlenmeyer flask) in a rotary shaker with agitation speed at180 rpm and temperature at 37°C.…”

“…Venkatadri reported that detergents including Tween series (Tween 20, 40, 60, and 80) and cholamidopropyl dimethylammonio propanesulfonate were able to protect both purified ligninase and crude ligninase in a cell-free culture against mechanical inactivation [11]. Grgic and Perdih demonstrated that addition of PEG and other polyoxyalkanes stimulated ligninase production [17]. Oleic acid and some phospholipids had a similar effect [18][19][20].…”

Effect of soya lecithin on the enzymatic system of the white-rot fungi Anthracophyllum discolor

“…Some studies have demonstrated that the presence of a surfactant may increase extracellular enzyme production in various filamentous fungi, including the white-rot fungi [13,15,22]. Additionally, its application in bioremediation processes may allow for an increase in mass transfer and the availability of hydrophobic organic contaminants [11].…”

“…Several studies have reported that the addition of some surfactants increases extracellular enzyme production in various filamentous fungi [13,15]. Nevertheless, the mechanism by which surfactants enhance enzyme production has not been established.…”

Análise enzimática de fungos lignocelulolíticos cultivados em vinhaça e bagaço de cana-de-açúcar

“…Isso se deve parcialmente por esse fungo ter sido um dos primeiros microrganismos celulolíticos a ser isolado na década de 1950 e também porque diversas cepas têm sido melhoradas tanto nos processos de produção de celulases industriais como na redução dos custos de produção, porém o ascomiceto T. reesei segundo Higuchi (1990), é incapaz de degradar lignina. Como os fungos de degradação branca pertencentes aos basidiomicetos são os mais eficientes e extensivos degradadores de lignina, o fungo P. chrysosporium já foi estudado extensivamente em diversos ensaios (BISALIA, 1984;KIRK, 1986;BERGUIN, 1994;DURÁN, 1997;CAMERON, 2000;FUJIAN, 2001;GRGIC, 2003) foi decidido trabalhar com os basidiomicetos do gênero Pleurotus. …”