Stimulation of Intracellular Free Ca2+ by Luteinizing Hormone in Isolated Bovine Luteal Cells

Davis, John S.

doi:10.1007/978-1-4684-5395-9_40

Cited by 11 publications

(7 citation statements)

References 18 publications

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“…FSH elevation of IPs displayed the same pattern. It would therefore appear that the cervical FSHR is associated with signal transduction pathways in a way similar to the accepted mechanism of LH action in the ovary and testes, i.e., activation of the second messenger pathways-adenylate cyclase [21] and phosphatidyl-inositol (PLC) [22,23]. The inhibitory effect of FSH on IPs and cAMP at postovulation occur when FSHR is low.…”

Section: Discussionmentioning

confidence: 98%

Follicle-Stimulating Hormone Receptor and Its Messenger Ribonucleic Acid Are Present in the Bovine Cervix and Can Regulate Cervical Prostanoid Synthesis1

Mizrachi¹,

Shemesh²

1999

Biology of Reproduction

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The hypothesis that FSH regulates the bovine cervical prostaglandin E(2) (PGE(2)) synthesis that is known to be associated with cervical relaxation and opening at the time of estrus was investigated. Cervical tissue from pre-estrous/estrous, luteal, and postovulatory cows were examined for 1) the presence of bovine (b) FSH receptor (R) and its corresponding mRNA and 2) the effect of FSH on the PGE(2) regulatory pathway in vitro. The presence of bFSHR mRNA in the cervix (maximal during pre-estrus/estrus) was demonstrated by the expression of a reverse transcription (RT) polymerase chain reaction (PCR) product (384 base pairs) specific for bFSHR mRNA and sequencing. Northern blotting revealed three transcripts (2.5, 3.3, and 3.8 kilobases [kb]) in cervix from pre-estrous/estrous cows. The level of FSHR (75 kDa) was significantly higher (p < 0.01) in Western blots of pre-estrous/estrous cervix than in other cervical tissues. There was a good correlation between the 75-kDa protein expression and its corresponding transcript of 2.55 kb throughout the estrous cycle as described by Northern blot analysis as well as RT-PCR. Incubation of FSH (10 ng/ml) with pre-estrous/estrous cervix resulted in a 3-fold increase in the expression of FSHR and a 2-fold increase in both G protein (alpha(s)) and cyclooxygenase II. FSH (5-20 ng/ml) significantly increased (p < 0.01) cAMP, inositol phosphate (p < 0.01), and PGE(2) (p < 0.01) production by pre-estrous/estrous cervix but not by cervix at the other stages. We conclude that bovine cervix at the time of the peripheral plasma FSH peak (pre-estrus/estrus) contains high levels of FSHR and responds to FSH by increasing the PGE(2) production responsible for cervical relaxation at estrus.

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Section: Discussionmentioning

confidence: 98%

Follicle-Stimulating Hormone Receptor and Its Messenger Ribonucleic Acid Are Present in the Bovine Cervix and Can Regulate Cervical Prostanoid Synthesis1

Mizrachi¹,

Shemesh²

1999

Biology of Reproduction

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“…Forty five micrograms of protein of soluble extract was analyzed for LH-R by Western blot using rat anti-LH-R antibody as previously described. 8 Western blot shows LH-R level in myometrial tissues from postovulatory (days 1-4), luteal (days [12][13][14][15][16], preestrus/estrus (days [18][19][20] myometrium. The histogram represents the relative optical density value at each stage of the cycle (n = 7) compared with the average optical density at preestrus/estrus.…”

Section: Figurementioning

confidence: 99%

“…Thus, binding of LH to the cervical and myometrial LH-R is associated with signal transduction pathways in a way similar to the accepted mechanism of action of LH in the ovary and testes, that is, activation of the second messenger adenylate cyclase 14 and phosphatidylinositol (PLC) pathways. [15][16][17] Furthermore, because LH also increased the expression of its own receptor, LH apparently acts at several sites to exert its action on the cervix and myometrium.…”

Section: Lh Activation Of Adenylate Cyclase and Inositol Phosphatementioning

confidence: 99%

Functional Importance of Bovine Myometrial and Vascular LH Receptors and Cervical FSH Receptors

Shemesh¹,

Mizrachi²,

Gurevich³

et al. 2001

Semin Reprod Med

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Bovine myometrium and cervix contain luteinizing hormone/human chorionic gonadotropin (LH/hCG) binding sites, LH receptor (LH-R) messenger RNA (mRNA), and LH-R protein. Expression of LH-R is dependent on the stage of the cycle. LH-R expression is high during the luteal phase but weak during the follicular phase. In both myometrium and cervix, LH activates both the adenylate cyclase and phospholipase C pathways, and the effect of LH on both pathways at each stage of the cycle is correlated with the amount of LH-R present in the tissue. Because activation of cyclic AMP (cAMP) is associated with myometrial quiescence, we suggest that LH activation of uterine cAMP could serve to keep the uterus quiescent during the luteal phase. On the other hand, in the uterine vein LH-R mRNA and LH-R are maximal during preestrus/estrus as opposed to the luteal phase. In the uterine vein, LH increases the expression of cyclooxygenase and production of both prostaglandin E2 (PGE2) and PGF2 alpha. Because PGF2 alpha is the physiological luteolytic signal in the cow, we suggest that this increase in prostaglandin production by the uterine vein is part of the physiological events leading to luteolysis. In addition to uterine LH-R, the bovine cervix at preestrus/estrus has high levels of follicle-stimulating hormone receptor (FSH-R) and its corresponding mRNA. As with LH-R, activation of FSH-R by FSH is associated with activation of a G protein-coupled receptor family that mediates the cAMP and inositol phosphate signaling pathways. Activation of these signaling pathways is associated with an increase in the expression of cyclooxygenase and production of PGE2. Because expression of the FSH receptor was maximal at the time of the FSH peak in the blood, we suggest a physiological role for FSH in the cervix relaxation and opening at estrus.

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“…Gonadotropin receptors in the uterus are associated with the same transduction pathways as in the gonads (23,24), and FSH stimulates the production of cAMP and inositol phosphate signaling pathways (18); accordingly, it encourages cyclooxygenase-2 (COX-2) to increase the emission of prostaglandin E 2 (PGE2), which has a role in relaxing myometrium (22,(25)(26)(27).…”

Section: Discussionmentioning

confidence: 99%

Spectrotemporal changes in electrical activity of myometrium due to recombinant follicle-stimulating hormone preparations follitropin alfa and beta

Çelik

Tağluk

Hasçalık

et al. 2008

Fertility and Sterility

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Stimulation of Intracellular Free Ca2+ by Luteinizing Hormone in Isolated Bovine Luteal Cells

Cited by 11 publications

References 18 publications

Follicle-Stimulating Hormone Receptor and Its Messenger Ribonucleic Acid Are Present in the Bovine Cervix and Can Regulate Cervical Prostanoid Synthesis1

Follicle-Stimulating Hormone Receptor and Its Messenger Ribonucleic Acid Are Present in the Bovine Cervix and Can Regulate Cervical Prostanoid Synthesis1

Functional Importance of Bovine Myometrial and Vascular LH Receptors and Cervical FSH Receptors

Spectrotemporal changes in electrical activity of myometrium due to recombinant follicle-stimulating hormone preparations follitropin alfa and beta

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