Stimulation of Interferon Production by Mannans

Lackovic, V; Borecký, L; Šikl, D.; Masler, L.; Bauer, Štefan

doi:10.3181/00379727-134-34902

Cited by 10 publications

(3 citation statements)

References 7 publications

(8 reference statements)

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“…According to Underhill and Ozinsky (2002), the observed increase in the phagocytic activity could be attributed to MOS binding by mannose receptors (MRs), whereas Borchers et al (2004) attributed the above to β-glucan binding by specific receptors on macrophages/monocytes and granulocytes, which increases the production of proinflammatory cytokines such as the tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, IL-8, IL-12, IFN-γ and IFN-β2 (Rasmussen and Seljelid 1991, Abel and Czop 1992, Che et al 2012). IL-12 seems to play a special role in this process by promoting type 1 T-helper cell (Th1) responses and cell-mediated immunity, and acting as a potent inducer of IFN-γ and IL-2 produced by Th1 cells (Lackovic et al 1970, Gately et al 1998, Ranta et al 2013. IFN-γ and IL-2 stimulate macrophage activation and differentiation into subpopulations of conventionally activated macrophages that are characterized by greater ability to ingest bacteria, produce ROS and other toxins and release antibacterial proteins such as defensins (Ganz 1987, Mosmann and Coffman 1989, Vetvicka et al 2008.…”

Section: Discussionmentioning

confidence: 99%

The effect of Biolex-MB40 on the phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes in lambs

Wójcik¹

2014

Polish Journal of Veterinary Sciences

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The objective of this study was to determine the effect of Biolex-MB40 on the phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes in lambs. The experimental material comprised 32 lambs aged 30 ± 3 days, divided into two equal groups: control and experimental. Experimental group animals were fed a diet supplemented with the Biolex-MB40 (Saccharomyces cerevisiae) in the amount of 3 g/kg of the concentrate. At the beginning of the experiment (day 0) and on experimental days 15, 30 and 60, blood was sampled from the jugular vein to determine and compare the phagocytic activity (PHAGOTEST) and oxidative metabolism (BURSTTEST) of peripheral blood granulocytes and monocytes by flow cytometry. Based on the results of an analysis of granulocyte and monocyte phagocytic activity, statistically higher levels of phagocytic activity were observed in the group of lambs administered Biolex-MB40 than in the control animals, expressed in terms of the percentage of phagocytic cells as well as mean fluorescence intensity. Biolex-MB40 also had a positive effect on the oxidative metabolism of both granulocytes and monocytes after stimulation with Escherichia coli bacteria and with PMA (4-phorbol-12-β-myristate-13-acetate), expressed in terms of the percentage of oxidative metabolism as well as mean fluorescence intensity.

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Section: Discussionmentioning

confidence: 99%

The effect of Biolex-MB40 on the phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes in lambs

Wójcik¹

2014

Polish Journal of Veterinary Sciences

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“…β-glucans enhance phenotypic and functional maturation of monocyte-derived dendritic cells and stimulate IL-12 production. An increase in IL-12 levels contributes to the differentiation of Th0 cells into Th1 cells, which leads to the production of typical Th1 cytokines, including IL-1β, IFN-γ, IL-2 and TNF-α, which play an important role in cell-mediated immunity [ 80 – 82 ]. Interferon γ and IL-2 stimulate the production of activated macrophages characterised by their greater ability to engulf and destroy bacteria [ 83 , 84 ].…”

Section: Discussionmentioning

confidence: 99%

Reactivity of the immunological system of rats stimulated with Biolex-Beta HP after cyclophosphamide immunosuppression

Wójcik¹

2014

cejoi

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The objective of this study was to determine the stimulating effect of the Biolex-Beta HP (β-1,3/1,6-D-glucan) dietary supplement on selected parameters of specific and non-specific humoral and cellular immunity in rats immunosuppressed with cyclophosphamide. The experimental material comprised 40 Wistar rats, divided into two equal groups: control and experimental. In the course of 3 successive days, the rats from the experimental group were administered cyclophosphamide intramuscularly at a rate of 50 mg/kg BW per day. On the 8th day of the experiment, 10 control and 10 experimental rats were sacrificed, and total protein and γ-globulin levels, lysozyme and ceruloplasmin activity were determined in the blood serum. The proliferative response of blood lymphocytes after stimulation with lipopolysaccharide or concanavalin A, respiratory burst activity and the potential killing activity of phagocytes were determined in whole heparinised blood. Starting on the 8th day of the experiment, the feed of the remaining rats from the experimental and control groups was supplemented for 14 consecutive days with Biolex-Beta HP at a rate of 50 mg/kg BW per day. On day 22, arterial blood samples were collected and immune parameters were determined. The results indicate that β-1,3/1,6-D-glucan has a positive effect on the analysed parameters of non-specific cellular and humoral immunity after cyclophosphamide-induced suppression. Nevertheless, the observed effect only marked a return to the norm, as most of the analysed parameters were merely restored to their initial levels, with the exception of lysozyme activity, which considerably exceeded the level noted before immunosuppression.

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“…Summarizing the information on immune activity and structure of the studied biopolymers we can conclude that the LY-EPS with predominant β-bonds in the mannan-type molecule has a stronger effect on the stimulation of IFN-γ in speci c immune cells relative to the more heterogeneous EPS containing in addition β-bonds and α-bonds in the molecules. The reported in vitro studies with peritoneal cells in mice have shown that some mannans and mannan-protein complexes stimulate the release of interferon in vivo and in vitro (Lackovic 1970). Mannans are thought to stimulate the release of IFN by a mechanism similar to that of endotoxins but in contrast, their toxicity was minimal.…”

Section: )mentioning

confidence: 99%

Chemical characterization and biological effect of exopolysaccharides synthesized by Antarctic yeasts Cystobasidium ongulense AL101 and Leucosporidium yakuticum AL102 on murine innate immune cells

Rusinova‐Videva

Ognyanov²,

Georgiev³

et al. 2022

World J Microbiol Biotechnol

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The current study aimed to investigate exopolysaccharides (EPSs) produced by two Antarctic yeasts isolated from soil and penguin feathers samples collected on Livingston Island (Antarctica). The strains were identi ed as belonging to the species Leucosporidium yakuticum (LY) and Cystobasidium ongulense (CO) based on a molecular genetic analysis. The EPS production was investigated at submerged cultivation. Different chemical, chromatographic, and spectral analyses were employed to characterize EPSs. LY accumulated 5.5g/L biomass and 4.0g/L EPS after 120 h of cultivation, while CO synthesis 2.1g/L EPS at the end of cultivation and biomass amount reached 5.5g/L. LY-EPS was characterized by a higher total carbohydrate content (80%) and a lower protein content (18%) by comparison with CO-EPS (62%, 30%). The LY-EPS mainly consisted of mannose (90 mol%), whereas CO-EPS had also glucose, galactose, and small amounts of uronic acids (8-5 mol%). Spectral analyses (FT-IR and 1D, 2D NMR) revealed that LY-EPS comprised a typical β-(1→4)-mannan. Branched (hetero)mannan, together with β/α-glucans constituted the majority of CO-EPS. Unlike LY-EPS, which had a high percentage of high molecular weight populations, CO-EPS displayed a large quantity of lower molecular weight fractions and a higher degree of heterogeneity. LY-EPS (at a concentration of 100 ng/mL) elevated signi cantly interferon IFN-γ production in splenic murine macrophages and NK cells. The results indicated that newly identi ed EPS might affect IFN-γ signaling and in turn, might enhance anti-infectious responses. The data obtained also revealed the potential of EPSs and yeasts for practical application in biochemical engineering and biotechnology.

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Stimulation of Interferon Production by Mannans

Cited by 10 publications

References 7 publications

The effect of Biolex-MB40 on the phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes in lambs

The effect of Biolex-MB40 on the phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes in lambs

Reactivity of the immunological system of rats stimulated with Biolex-Beta HP after cyclophosphamide immunosuppression

Chemical characterization and biological effect of exopolysaccharides synthesized by Antarctic yeasts Cystobasidium ongulense AL101 and Leucosporidium yakuticum AL102 on murine innate immune cells

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