Stimulation of IL-8 Production byAralia cordateLectin in Human Colon Carcinoma Caco-2 Cells

Cytokine Responses of Intestinal Epithelial-Like Caco-2 Cells to Non-Pathogenic and Opportunistic Pathogenic Yeasts in the Presence of Butyric Acid

“…Human-derived IECs and/or IEC lines (Caco-2, HT-29 and T84) have been shown to secrete cytokines, eicosanoids, and/or antimicrobial peptides in response to such stimuli as pathogenic and non-pathogenic bacterial cells [1][2][3][4][5][6] and vegetable lectins. 7,8) The exposure of IEC lines, including Caco-2, to butyric acid, a short chain fatty acid produced by bacterial carbohydrate fermentation in the mammalian intestine, 9,10) has been found to alter the cytokine secretion, NF-B activation, arachidonic cascade, cell cycles, cell differentiation and alkaline phosphatase activity of these cells. [11][12][13][14][15][16] We have previously shown that IEC-like Caco-2 cells cultured in a medium containing 10 mM butyric acid secreted interleukin-8 (IL-8/CXCL-8) in response to both non-pathogenic S. cerevisiae and the opportunistic pathogenic C. albicans.…”

Effects of Japanese mistletoe lectin on cytokine gene expression in human colonic carcinoma cells and in the mouse intestine

“…Although we reported an apoptosis-inducing effect of ML-J on cancer cells (21), other biological activities have remained unknown. Since immunomodulatory properties of mistletoe lectins have been documented (2,5,22) and since we and others have shown that certain lectins stimulate cytokine expression using Caco-2 cells, a human intestinal cell line derived from a colon adenocarcinoma (20,32), we examined the effects of ML-J on cytokine expression in these cells and in animal experiments. In the present study, we show that ML-J stimulates the gene expression of proinflammatory cytokines, interleukin (IL)-8, tumor necrosis factor (TNF)-α and interleukin (IL)-6 in Caco-2 cells, and TNF-α and IL-6 in the mouse duodenum.…”