Cytokine Responses of Intestinal Epithelial-Like Caco-2 Cells to Non-Pathogenic and Opportunistic Pathogenic Yeasts in the Presence of Butyric Acid

Saegusa, Shizue; Totsuka, Mamoru; Kaminogawa, Shuichi; Hosoi, Tomohiro

doi:10.1271/bbb.70172

Cited by 34 publications

(30 citation statements)

References 45 publications

(42 reference statements)

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“…In the present study, and corroborating other authors, C. tropicalis yeasts were able to adhere to and to damage all three epithelial cell lines used (Bendel & Hostetter, 1993;Negri et al, 2010a;Saegusa et al, 2007). Furthermore C. tropicalis showed a range of expression profiles of SAP genes (Silva et al, 2011;Zaugg et al, 2001), although in a strain-and cell-line-dependent manner (Negri et al, 2010b;Okawa et al, 2008).…”

Section: Cell Linesupporting

confidence: 90%

“…Percentage inhibition of cell activity (±SD) Percentage cell damage (±SD) Several studies have been performed to clarify the behaviour of Candida species in the adhesion process, colonization and infection using human cell lines as a study model for Candida pathogenesis ex vivo (Bendel, 2003;Negri et al, 2010b;Pacheco et al, 2007;Saegusa et al, 2007;Sohn et al, 2006). However, compared to Candida albicans, only a few investigations have been performed to assess the virulence of C. tropicalis, particularly in the presence of different human cell lines.…”

Section: Cell Linementioning

confidence: 99%

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An in vitro evaluation of Candida tropicalis infectivity using human cell monolayers

Negri

Botelho

Silva

et al. 2011

Journal of Medical Microbiology

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The aim of this study was to investigate the interaction of Candida tropicalis with three different human cell lines: TCC-SUP (epithelial cells from urinary bladder), HeLa (epithelial cells from cervical carcinoma) and Caco-2 (epithelial cells from colorectal adenocarcinoma). In particular we sought to assess the degree of cell damage and activity reduction induced by C. tropicalis adhesion and the role of secreted aspartyl proteinase (SAP) gene expression in this process. Two C. tropicalis strains were used: the reference strain ATCC 750 and a clinical isolate from urine (U69). The ability of C. tropicalis to adhere to a confluent layer of human cells was determined using an adaptation of the crystal violet staining method; cell damage and cell activity inhibition induced by the adhesion of C. tropicalis were assessed by measuring lactate dehydrogenase and tetrazolium salt (MTS) reduction, respectively. C. tropicalis SAP gene expression was determined by real-time PCR. Both C. tropicalis strains were able to adhere to the different human cells, although in a strain-and cell-line-dependent manner. Concerning the cellular response to C. tropicalis, the highest inhibition of cell activity was obtained for Caco-2, followed by TCC-SUP and HeLa cells. The highest percentage of cell damage (around 14 %) was observed for TCC-SUP cells in contact with the U69 isolate and for Caco-2 in contact with the reference strain. Real-time PCR analysis revealed a wide range of expression profiles of SAP genes for both C. tropicalis strains in contact with the different types of epithelial cells. SAPT3 was the gene expressed at the highest level for both C. tropicalis strains in contact with the three human epithelial cell lines. The results highlight that the response of human cells to C. tropicalis adhesion, as well as production of SAPs, is dependent on both the strain and the epithelial cell line.

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Section: Cell Linesupporting

confidence: 90%

Section: Cell Linementioning

confidence: 99%

An in vitro evaluation of Candida tropicalis infectivity using human cell monolayers

Negri

Botelho

Silva

et al. 2011

Journal of Medical Microbiology

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“…These results suggest that non-pathogenic and opportunistic pathogenic yeast present transiently or commensally in the human intestine differentially promote intestinal immune responses of neutrophils as well as intestinal epithelial cells. 5,6) Moreover, in our experimental model, the intensities of cytokine-inducible activities of certain microbes and/or related molecules do not necessarily correlate with pathogenicity in vivo.…”

Section: Discussionmentioning

confidence: 80%

“…5,6) Several types of non-pathogenic commensal and/or probiotic Escherichia coli, Lactobacillus sakei, cell extract fractions of Streptococcus thermophilus, [7][8][9][10] and pathogenic Salmonella dublin, Yersinia enterocolitica, Shigella dysenteniae and Listeria monocytogenes 11,12) stimulate IL-8 production from intestinal epithelial cells. IL-8 is a CXC chemokine displaying chemotactic activity for neutrophils (polymorphonuclear leukocytes; PMNs).…”

mentioning

confidence: 99%

Saccharomyces cerevisiaeandCandida albicansStimulate Cytokine Secretion from Human Neutrophil-Like HL-60 Cells Differentiated with Retinoic Acid or Dimethylsulfoxide

Saegusa

Totsuka

Kaminogawa

et al. 2009

Bioscience, Biotechnology, and Biochemistry

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“…10,11) For the future application to fermented foods including the yeast, we have to confirm the stimulation of intestinal immunity by ingestion of the foods.…”

mentioning

confidence: 99%

Isolation of diploid baker’s yeast capable of strongly activating immune cells and analyses of the cell wall structure

Takada

Mizobuchi

Kato

et al. 2014

Bioscience, Biotechnology, and Biochemistry

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Cytokine Responses of Intestinal Epithelial-Like Caco-2 Cells to Non-Pathogenic and Opportunistic Pathogenic Yeasts in the Presence of Butyric Acid

Cited by 34 publications

References 45 publications

An in vitro evaluation of Candida tropicalis infectivity using human cell monolayers

An in vitro evaluation of Candida tropicalis infectivity using human cell monolayers

Saccharomyces cerevisiaeandCandida albicansStimulate Cytokine Secretion from Human Neutrophil-Like HL-60 Cells Differentiated with Retinoic Acid or Dimethylsulfoxide

Isolation of diploid baker’s yeast capable of strongly activating immune cells and analyses of the cell wall structure

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