Stimulation of Glycosaminoglycan Biosynthesis by Umbilical Cord Serum of Newborns Delivered by Mothers with EPH Gestosis (Preeclampsia)

Romanowicz, Lech; Bańkowski, Edward; Jaworski, Stefan

doi:10.1159/000055936

Cited by 4 publications

(3 citation statements)

References 24 publications

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“…The increased levels of placental CP in PE may result in enhanced ferro-oxidative activity in this tissue, thereby oxidizing excess ferrous iron to the less toxic ferric form [49]. The syncytial CP induced by severe PE, is important in an endogenous cellular program to mitigate the damaging effects of subsequent reperfusion injury [49]. SOD is a primary antioxidant enzyme whose expression is essential for life in oxygen.…”

Section: Discussionmentioning

confidence: 99%

Comparative Proteome Profile of Human Placenta from Normal and Preeclamptic Pregnancies

et al. 2013

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To better understand the molecular mechanisms involved in pathological development of placenta in preeclampsia, we used LC-MS/MS to construct a large-scale comparative proteome profile of human placentas from normal and preeclamptic pregnancies. A total of 2636 proteins were detected in human placentas, and 171 different proteins were definitively identified between control and preeclamptic placentas. Further bioinformatics analysis indicated that these differentially expressed proteins correlate with several specific cellular processes which occur during pathological changes of preeclamptic placenta. 6 proteins were randomly selected to verify their expression patterns with Western blotting. Of which, 3 proteins’ cellular localizations were validated with immunohistochemistry. Elucidation of how protein-expression changes coordinate the pathological development would provide researchers with a better understanding of the critical biological processes of preeclampsia and potential targets for therapeutic agents to regulate placenta function, and eventually benefit the treatment of preeclampsia.

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Section: Discussionmentioning

confidence: 99%

Comparative Proteome Profile of Human Placenta from Normal and Preeclamptic Pregnancies

et al. 2013

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“…The slices of preeclamptic arterial wall incubated in vitro demonstrated increased biosynthesis and reduced degradation of this protein [3] . Furthermore, preeclamptic umbilical cord (UC) serum stimulated fibroblast division in culture [4] and specifically enhanced biosynthesis of collagen [5] and sulfated glycosaminoglycans [6] in these cells. We found that preeclampsia is associated with an increase of IGF-1 concentration in the umbilical cord blood and an elevation in IGF-binding protein (IGFBP) contents in the UCA wall.…”

Section: Introductionmentioning

confidence: 99%

The Inhibitory Effect of Preeclamptic Umbilical Cord Blood Serum on Matrix Metalloproteinase-1 in Arterial Slices Incubated in vitro

Galewska

Romanowicz²,

Gogiel³

et al. 2006

Pathobiology

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Background: Our previous studies demonstrated that preeclampsia is accompanied by significant alterations in the amounts of peptide growth factors in the umbilical cord serum. Some of these factors (especially IGF-1) are known as regulators of collagen metabolism. The umbilical cord arteries (UCAs) of newborns delivered by mothers with preeclampsia contain more than twice the amount of collagen in comparison to newborns delivered by healthy mothers. A significant role in collagen degradation is attributed to matrix metalloproteinase (MMP)-1 (collagenase 1) and tissue inhibitors of metalloproteinases (TIMPs). Objective: To compare the effects of umbilical cord (UC) blood serum of control and preeclamptic newborns on the content and activity of MMP-1, TIMP-1 and TIMP-2 in UCA wall slices incubated in vitro. Methods: Polyacrylamide gel electrophoresis (PAGE) followed by Western immunoblotting allowed to detect MMP-1 as well as TIMP-1 and TIMP-2. The amounts of MMP-1, TIMP-1 and TIMP-2 in UCA slices were measured by immunoenzymatic method (ELISA). MMP-1 activity in the arterial wall was measured using a collagenase-1-specific substrate. Results: Western immunoblot analyses detected MMP-1, TIMP-1 and TIMP-2 in the incubation fluids and in extracts from the UCA wall. Both 43- and 55-kDa (a zymogen) bands of MMP-1 were visible. The control UC serum stimulated both the amount as well as actual and potential activities of MMP-1 in the arterial wall in a time-dependent manner. In contrast to controls, the preeclamptic serum did not exert such an effect. Conclusions: The small amount and low activity of MMP-1 accompanied by elevated amounts of TIMPs (especially TIMP-1) decelerate the degradation and enhance the accumulation of collagen in the preeclamptic UCA wall.

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“…It remains to be determined if scavenging of circulating BMP9 by sEng is important in PE and regulation of hypertension [39]. On the contrary, the syncytial CP induced by severe PE, is an important endogenous cellular program to mitigate the damaging effects of subsequent reperfusion injury by enhancing ferro-oxidative activity oxidizing excess Fe 2+ to the less toxic Fe 3+ form [40]. Down-regulation of SOD, uridine diphosphate-glucose 6-dehydrogenase and prenylcysteine oxidase 1 proteins are associated with oxidative stress explaining the maternal vascular disease and placental dysfunction [41].…”

Section: Maternal Blood or Placental Tissue Proteomementioning

confidence: 99%

Preeclampsia at the molecular level

El-Desouki¹

2015

Appl Med Res

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In clinical practice, we found despite the promise of some biomarkers in identifying women at increased risk for preeclampsia (PE) is still challenging! PE is a pregnancy-specific syndrome causes substantial maternal and fetal/neonatal morbidity and mortality worldwide. Despite decades of research, the etiology is incompletely understood and hence the ability of clinicians to predict PE prior to the onset of symptoms has not improved significantly. In this review, we will explore potential future areas of research underlying the pathophysiology of PE at the molecular level and potential biomarkers evolving for early prediction and diagnosis hoping to suggest novel therapeutic interventions

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Stimulation of Glycosaminoglycan Biosynthesis by Umbilical Cord Serum of Newborns Delivered by Mothers with EPH Gestosis (Preeclampsia)

Cited by 4 publications

References 24 publications

Comparative Proteome Profile of Human Placenta from Normal and Preeclamptic Pregnancies

Comparative Proteome Profile of Human Placenta from Normal and Preeclamptic Pregnancies

The Inhibitory Effect of Preeclamptic Umbilical Cord Blood Serum on Matrix Metalloproteinase-1 in Arterial Slices Incubated in vitro

Preeclampsia at the molecular level

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