Comparative Proteome Profile of Human Placenta from Normal and Preeclamptic Pregnancies

Abstract

To better understand the molecular mechanisms involved in pathological development of placenta in preeclampsia, we used LC-MS/MS to construct a large-scale comparative proteome profile of human placentas from normal and preeclamptic pregnancies. A total of 2636 proteins were detected in human placentas, and 171 different proteins were definitively identified between control and preeclamptic placentas. Further bioinformatics analysis indicated that these differentially expressed proteins correlate with several specific cellular processes which occur during pathological changes of preeclamptic placenta. 6 proteins were randomly selected to verify their expression patterns with Western blotting. Of which, 3 proteins’ cellular localizations were validated with immunohistochemistry. Elucidation of how protein-expression changes coordinate the pathological development would provide researchers with a better understanding of the critical biological processes of preeclampsia and potential targets for therapeutic agents to regulate placenta function, and eventually benefit the treatment of preeclampsia.

Derlin-1 promotes ubiquitylation and degradation of the epithelial Na+ channel, ENaC

“…The LC-MS/MS was operated in the positive ion model as described previously. The MS/MS spectra acquired from precursor ions were submitted to Maxquant (version 1.2.2.5), and search parameters were followed, as described before (Wang et al, 2013).…”

HRD1-Mediated IGF-1R Ubiquitination Contributes to Renal Protection of Resveratrol in db/db Mice

“…Cell lysates were immunoprecipitated with anti-HRD1 antibody. Protein digestion, labeling, mass spectrometry data acquisition, and identification were completed in the analysis center of Nanjing Medical University, as described before (25). In brief, the labeled peptides were analyzed on a LTQ-Orbitrap instrument (Thermo Fisher Scientific) connected to a Nano ACQUITY UPLC system via a nanospray source.…”

“…The liquid chromatography and tandem mass spectrometry (LC-MS/MS) was operated in the positive ion model as described previously. The tandem mass spectrometry spectra acquired from precursor ions were submitted to Maxquant (version 1.2.2.5), and search parameters were followed, as described before (25).…”

Proteomics Analysis of Preeclampsia, a Systematic Review of Maternal and Fetal Compartments

“…Mitochondria trifunctional enzyme subunit alpha and beta are only significantly altered in placenta tissue although with conflicting results. This conflict may result from differences in sampling as Wang et al [28,29] (Table 1-3) did not specify the control group use nor did they include proteinuria as a diagnostic for PE, while Shi et al [30] (Table 1-3) defined both. Apolipoprotein A-II was consistently down-regulated in 2/2 studies and apolipoprotein E (apoE) was ambiguous.…”