Stimulation of endometrium embryo transfer (SEET): injection of embryo culture supernatant into the uterine cavity before blastocyst transfer can improve implantation and pregnancy rates

Goto, Sakae; Kadowaki, Takashi; Hagiwara, Hiromi; Kokeguchi, Shoji; Shiotani, Masahide

doi:10.1016/j.fertnstert.2007.01.011

Cited by 29 publications

(27 citation statements)

References 24 publications

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“…In our study, the pregnancy rate of the BT group was 66.6%, which is similar to the findings of Goto et al (64%) [8]. As we had used high-grade blastocysts and helped blastocysts to hatch, the implantation rates in BT and SEHB groups were not significantly different, but they were statistically different in the study of Goto et al (64 vs. 92%) [10].…”

Section: Discussionsupporting

confidence: 88%

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Stimulation of the endometrium with high-grade blastocyst culture supernatant (SEHB) can improve pregnancy outcome for couples undergoing intracytoplasmic sperm injection (ICSI): a randomized clinical trial

Tehraninejad

Tanha

Ghajarzadeh

et al. 2011

Arch Gynecol Obstet

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Section: Discussionsupporting

confidence: 88%

“…It was observed that the implantation and pregnancy rates were higher in the ECS group than the BT only group (pregnancy rates 86 vs. 48%) [8], however, they used a media produced by both high and poor quality embryos, and did not follow-up their cases until delivery.…”

Section: Introductionmentioning

confidence: 99%

Stimulation of the endometrium with high-grade blastocyst culture supernatant (SEHB) can improve pregnancy outcome for couples undergoing intracytoplasmic sperm injection (ICSI): a randomized clinical trial

Tehraninejad

Tanha

Ghajarzadeh

et al. 2011

Arch Gynecol Obstet

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“…In our recent study, we presented SEET, an innovative method of embryo transfer based on the concept of embryo-dependent induction of endometrial receptivity, and demonstrated the effectiveness of SEET for patients who had a history of one or more unsuccessful BT or two-step (consecutive double) embryo transfers (10). In the present study, we demonstrated that SEET yielded significantly higher implantation rates and PRs for first-time ART patients who had a single high-grade blastocyst transferred.…”

Section: Discussionmentioning

confidence: 99%

“…In blastocyst transfer (BT), cross-talk is absent until the blastocysts are transferred and the pregnancy rate (PR) using BT is limited to around 40% (9). Accordingly, to improve the pregnancy outcome of BT, we developed an innovative procedure of embryo transfer based on knowledge regarding the embryonic modulation of endometrial receptivity, named stimulation of endometrium embryo transfer (SEET) (10). In SEET, ECS was injected into the uterine cavity before BT to stimulate the endometrium and provide an optimum environment for implantation of forthcoming blastocysts.…”

mentioning

confidence: 99%

Stimulation of endometrium embryo transfer can improve implantation and pregnancy rates for patients undergoing assisted reproductive technology for the first time with a high-grade blastocyst

Goto

Kadowaki

Hagiwara

et al. 2009

Fertility and Sterility

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“…Kenaikan angka implantasi pada kelompok perlakuan juga oleh Goto et al (2007). Angka kebuntingan pada kelompok perlakuan meningkat dan kelompok kontrol masing-masing adalah 87 dan 48%.…”

Section: Gambar 1 Embrio Dalam Lumen Endotelial (Le) Mencitunclassified

Injeksi Media Kultur Embrio Supernatan Dalam Uterus Untuk Meningkatkan Angka Implantasi Embrio Pada Mencit

et al. 2013

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ABSTRAKPenelitian ini bertujuan mengetahui pengaruh injeksi media kultur embrio supernatan ke dalam uterus mencit 2 hari sebelum transfer embrio (TE) terhadap angka implantasi, yang diindikasikan oleh adanya embrio dan leukemia inhibitory factor (LIF) pada uterus. Mencit jenis Swiss Albino dibagi menjadi 2 kelompok yakni kelompok perlakuan dan kelompok kontrol, masing-masing terdiri atas 30 mencit. Kelompok perlakuan mendapat injeksi media kultur embrio supernatan (MKES) dalam uterus 2 hari sebelum TE sedangkan kelompok kontrol mendapat injeksi media kultur embrio (MKE) dalam uterus 2 hari sebelum TE. Dua hari setelah TE (h6 kebuntingan), mencit diperfusi dengan menggunakan larutan para formaldehid 4% dan diambil uterusnya. Uterus diproses untuk blok parafin dan dipotong dengan mikrotom setebal 5 µm. Preparat dideparafinisasi dan diproses imunohistokimia dengan kit ABC dan antibodi LIF. Preparat diperiksa di bawah mikroskop untuk melihat implantasi embrio, distribusi LIF, dan jumlah LIF di desidua dan non-desidua. Angka implantasi antara kelompok perlakuan dan kelompok kontrol masing-masing adalah 52,77 dan 40,88% (P<0,05). Jumlah LIF desidua pada kelompok perlakuan dan kelompok kontrol masing-masing adalah 12,83 dan 8,83 (P<0,05) sedangkan jumlah LIF di non-desidua antara kelompok perlakuan dan kelompok kontrol masing-masing adalah 8,00 dan 4,50. Dapat disimpulkan bahwa injeksi media kultur embrio supernatan 2 hari sebelum TE dapat meningkatkan angka implantasi pada mencit. ____________________________________________________________________________________________________________________ Kata kunci: kultur embrio supernatan, angka implantasi, leukemia inhibitory factor ABSTRACTThe research was conducted to examine the effect of supernatant embryonic media culture injection into mice uterine at 2 days prior to embryo transfer implantation rate, indicated by the presence of implanted embryos and leukemia inhibitory factor (LIF) PENDAHULUANKomunikasi antara embrio dan endometrium (crosstalk) memegang peranan penting dalam keberhasilan implantasi dan kebuntingan. Hal ini dimulai sejak terjadinya embrio dan perjalanan dari saluran tuba Fallopii sampai implantasi pada endometrium. Embrio menghasilkan beberapa faktor selama perkembangannya dan memberi sinyal keberadaannya kepada organ maternal. Dalam 1-2 hari setelah fertilisasi dapat dideteksi adanya early pregnancy factor (EPF) pada sirkulasi darah maternal yang mempunyai sifat immunosuppresive dan berhubungan dengan proliferasi sel dan pertumbuhan (Fan dan Zheng, 1997).Saat terjadinya implantasi embrio, endometrium harus dalam keadaan kondusif untuk memungkinkan perlekatan, implantasi, dan pertumbuhan awal plasenta.Salah satu sinyal dari embrio yang memodulasi reseptor endometrium adalah human chorionic gonadotropin (hCG). Hormon hCG diproduksi oleh blastosis pada hari ke 7-8 pasca fertilisasi. Substansi lain yang disekresi embrio sebelum implantasi adalah platelet activating factor (PAF) yang mungkin bagian dari aktivitas immunosuppressive untuk menginduksi to...

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Stimulation of endometrium embryo transfer (SEET): injection of embryo culture supernatant into the uterine cavity before blastocyst transfer can improve implantation and pregnancy rates

Cited by 29 publications

References 24 publications

Stimulation of the endometrium with high-grade blastocyst culture supernatant (SEHB) can improve pregnancy outcome for couples undergoing intracytoplasmic sperm injection (ICSI): a randomized clinical trial

Stimulation of the endometrium with high-grade blastocyst culture supernatant (SEHB) can improve pregnancy outcome for couples undergoing intracytoplasmic sperm injection (ICSI): a randomized clinical trial

Stimulation of endometrium embryo transfer can improve implantation and pregnancy rates for patients undergoing assisted reproductive technology for the first time with a high-grade blastocyst

Injeksi Media Kultur Embrio Supernatan Dalam Uterus Untuk Meningkatkan Angka Implantasi Embrio Pada Mencit

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