Stimulation of cephalosporin C production in Acremonium chrysogenum M35 by glycerol

Utilization of algal sugars and glycerol for enhanced cephalosporin C production by Acremonium chrysogenum M35

“…Thus, in order to produce a high concentration of CPC in the early stage of production, 6% glycerol was added to the medium (Shin et al . ).…”

“…1(f) indicates that the swollen hyphal fragment was relatively large compared with those of Fig. 1(d or e), accelerating its differentiation, thus increasing the CPC production (Kim et al 2007;Shin et al 2010).…”

Eco-design and evaluation for production of 7-aminocephalosporanic acid from carbohydrate wastes discharged after microalgae-based biodiesel production

“…Additional materials (Chemical market report, 2010; Evangelista et al, 1998) such as 0.3% KH 2 PO 4 (3.0 $/kg), 0.5% K 2 HPO 4 (3.0 $/kg), 0.8% (NH 4 ) 2 SO 4 (0.132 $/kg), and 0.4% trace elements (2.0 $/kg) were added to the culture medium to support the cell growth (Shin et al, 2010(Shin et al, , 2011. The saccharified media and additional materials were transferred to a fermentor (P-5) to start fermentation.…”

Utilization of glycerol as cysteine and carbon sources for cephalosporin C production by Acremonium chrysogenum M35 in methionine-unsupplemented culture

“…Before 0.5% (w/v) CaCO 3 was added to the medium, the pH was adjusted to 7.0 with 1 N NaOH prior to sterilization. Previously, we demonstrated that the maximal cephalosporin C was produced, when 4% glycerol was used as a carbon source (Shin et al, 2010). Therefore, the effects of glycerol on cystathionine-␥-lyase activity, aminoadipic acid, valine and cysteine were evaluated by adding 4% (v/v) glycerol to the main medium.…”

“…With the corresponding reduction in price, glycerol is now an attractive feedstock for the production of useful chemicals. In a previous study, we showed that the hyphal fragments of A. chrysogenum are differentiated into arthrospores when cephalosporin C production is increased by the addition of glycerol (Shin et al, 2010). However, the beneficial effect of glycerol on cephalosporin C production is not fully understood yet.…”