Stimulation of bone regeneration following the controlled release of water-insoluble oxysterol from biodegradable hydrogel

Saito, Takashi; Li, Andrew; Sato, Keisuke; Tabata, Yasuhiko; Jarrahy, Reza

doi:10.1016/j.biomaterials.2014.03.018

Cited by 37 publications

(27 citation statements)

References 19 publications

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“…Large calvarial bone defects often arise from trauma, skeletal diseases, congenital malformations and tumor resections [1]. The repair of critical-sized bone defects (CSD) remains a challenge in reconstructive surgeons and usually requires urgent and effective handling, large-sized replacement and restoration of the mechanical and biological functions of bone [2].…”

Section: Introductionmentioning

confidence: 99%

Controlled dual delivery of BMP-2 and dexamethasone by nanoparticle-embedded electrospun nanofibers for the efficient repair of critical-sized rat calvarial defect

et al. 2015

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Section: Introductionmentioning

confidence: 99%

Controlled dual delivery of BMP-2 and dexamethasone by nanoparticle-embedded electrospun nanofibers for the efficient repair of critical-sized rat calvarial defect

et al. 2015

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“…Additionally, ST was water-solubilized with the LAo-g-gelatin, which is commonly used to form micelles 12,20,21) .…”

Section: Introductionmentioning

confidence: 99%

Controlled release of simvastatin from biodegradable hydrogels promotes odontoblastic differentiation

et al. 2015

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The objective of this study was to investigate the odontoblastic differentiation of dental pulp stem cells (DPSC) by biodegradable hydrogels incorporating simvastatin micelles, both in vitro and in vivo. Simvastatin (ST) was incorporated into the micelles of gelatin grafted with L-lactic acid oligomers (LAo) to allow water-solubilization. The simvastatin-LAo-grafted gelatin (LAo-g-gelatin) micelles were mixed with gelatin, followed by chemical crosslinking to form gelatin hydrogels (ST Mi/GH). The ST Mi were released from the gelatin hydrogel granules (GH) through enzymatic degradation. The ST Mi enhanced alkaline phosphatase activity, calcium deposition, and bone morphogenic protein-2 secretion of DPSC. When implanted subcutaneously into mice, the ST Mi/GH treated group exhibited increased dentin sialoprotein and calcium deposition, compared with those treated with GH plus free ST. It is possible to achieve odontoblastic differentiation of DPSC through the controlled release of ST from GH.

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“…For the degradation test we considered a modification of the ASTM F1634-95 standard [26], hence, the substitutes were immersed in a solution of concentrated ionic salts or SBF with a pH of 7, following the protocol described by Kokubo and Takadama [27]; afterwards, the samples were incubated at 37 °C for 28 days. After we removed the samples from the solution and dried them as described above, we measured degradation by recording their mass on days 0, 1, 7, 14, 21, and 28, to observe the weight loss trend and calculate the percentage of degradation using equation (2). It should be noted that day 0 is the start day, thus the mass corresponds to the initial mass of the sample.…”

Section: In Vitro Degradation and Water Uptakementioning

confidence: 99%

“…Currently, numerous types of grafts, classified according to their origin, are use clinically: autografts from the same patient, allografts from the same species, xenografts from other species, such as deproteinized bovine bone, and alloplastic bone substitutes that are synthetically manufactured [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

“…However, they have disadvantages in terms of the extraction site, which usually is in the iliac crest or other bones such as tibia and femur; this extraction involves double surgery, increase pain, morbidity of the extraction site, and quantity of bone available according to the amount of tissue needed to correct the defect [1,2]. Allografts provide biocompatibility properties, different reconstruction options, and replacement of bone parts.…”

Section: Introductionmentioning

confidence: 99%

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Degradation, water uptake, injectability and mechanical strength of injectable bone substitutes composed of silk fibroin and hydroxyapatite nanorods

Buitrago-Vásquez

Orozco

2018

Rev. Fac. Ing.

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Injectable bone substitutes are generally composite materials capable of being extruded through a device; they consist of a solid phase inside a matrix that allows the easy movement of particles. Injectable bone substitutes have the advantage of allowing its application in situ without the need of invasive surgical techniques, guaranteeing a good recovery; for this reason, they are a promising alternative to replace conventional techniques to repair bone defects. Conventional techniques include the use of allografts and autografts, which often cause adverse reactions, and are disadvantageous for both the patient and the doctor. Furthermore, there are no reports regarding bone substitute development in Colombia, creating the necessity to research composite materials that could become injectable bone substitutes. In this study, we manufactured injectable bone substitutes with hydroxyapatite and calcium phosphate, which is most similar to bone tissue, and synthesized them in nanorods with shape and size similar to the natural hydroxyapatite found inside the body. Additionally, we used extracted silk fibroin from silkworm cocoons of Bombyx mori, a natural polymer of protein nature with high mechanical properties and excellent biocompatibility. For the materials manufactured, we evaluated degradation, in a simulated body fluid (SBF) at normal body temperature, water uptake, injectability and mechanical strength. The manufactured bone substitutes showed good degradation and water uptake properties, an approximate 97% injectability, and low mechanical resistance, indicating promising properties to be used as an injectable bone substitute.

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Stimulation of bone regeneration following the controlled release of water-insoluble oxysterol from biodegradable hydrogel

Cited by 37 publications

References 19 publications

Controlled dual delivery of BMP-2 and dexamethasone by nanoparticle-embedded electrospun nanofibers for the efficient repair of critical-sized rat calvarial defect

Controlled dual delivery of BMP-2 and dexamethasone by nanoparticle-embedded electrospun nanofibers for the efficient repair of critical-sized rat calvarial defect

Controlled release of simvastatin from biodegradable hydrogels promotes odontoblastic differentiation

Degradation, water uptake, injectability and mechanical strength of injectable bone substitutes composed of silk fibroin and hydroxyapatite nanorods

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