Stimulation of bone marrow cells and bone formation by nacre: in vivo and in vitro studies

Lamghari, Meriem; Almeida, Maria; Berland, Sophie; Huet, H.; Laurent, A.; Milet, Christian; Lopez, Évelyne

doi:10.1016/s8756-3282(99)00141-6

Cited by 122 publications

(48 citation statements)

References 9 publications

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“…Besides regulating the mineralization process of nacre, water-soluble matrix (WSM) contributes to its biological activities [3,4]. These WSM molecules are evidently involved in cell recruitment, differentiation and stimulation to produce mineralized tissues [5,6]. Due to beneficial biological activities, nacre finds uses in several traditional pharmaceutical preparations, by stimulating bone growth and enhancing bone density.…”

Section: Introductionmentioning

confidence: 99%

Water Soluble Bioactives of Nacre Mediate Antioxidant Activity and Osteoblast Differentiation

2013

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The water soluble matrix of nacre is a proven osteoinductive material. In spite of the differences in the biomolecular compositions of nacre obtained from multiple species of oysters, the common biochemical properties of those principles substantiate their biological activity. However, the mechanism by which nacre stimulates bone differentiation remains largely unknown. Since the positive impact of antioxidants on bone metabolism is well acknowledged, in this study we investigated the antioxidant potential of a water soluble matrix (WSM) obtained from the nacre of the marine oyster Pinctada fucata, which could regulate its osteoblast differentiation activity. Enhanced levels of ALP activity observed in pre-osteoblast cells upon treatment with WSM, suggested the induction of bone differentiation events. Furthermore, bone nodule formation and up-regulation of bone differentiation marker transcripts, i.e. collagen type-1 and osteocalcin by WSM confirmed its ability to induce differentiation of the pre-osteoblasts into mature osteoblasts. Remarkably, same WSM fraction upon pre-treatment lowered the H2O2 and UV-B induced oxidative damages in keratinocytes, thus indicating the antioxidant potential of WSM. This was further confirmed from the in vitro scavenging of ABTS and DPPH free radicals and inhibition of lipid peroxidation by WSM. Together, these results indicate that WSM poses both antioxidant potential and osteoblast differentiation property. Thus, bioactivities associated with nacre holds potential in the development of therapeutics for bone regeneration and against oxidative stress induced damages in cells.

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Section: Introductionmentioning

confidence: 99%

Water Soluble Bioactives of Nacre Mediate Antioxidant Activity and Osteoblast Differentiation

2013

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“…Other subsequent experiments have tested nacre’s osteogenic power in few laboratory animals, such as sheep and have reported similar dramatic osteostimulatory effects [163]. In 12 weeks a defect excavated in the lumbar vertebra and filled with nacre powder transformed into spongy bone while the nacre was seen to have dissolved [163]. In comprehending these results we cannot rule out some osteogenic effect from the nacre surface structure itself such as the micro- and nanotopography.…”

Section: Biomedical Applications Of Marine Productsmentioning

confidence: 96%

“…There was no inflammation and fresh bone had been generated as the nacre resorbed. Other subsequent experiments have tested nacre’s osteogenic power in few laboratory animals, such as sheep and have reported similar dramatic osteostimulatory effects [163]. In 12 weeks a defect excavated in the lumbar vertebra and filled with nacre powder transformed into spongy bone while the nacre was seen to have dissolved [163].…”

Section: Biomedical Applications Of Marine Productsmentioning

confidence: 99%

“…In the study by Lamghari et al it was proposed that the matrix peptides stimulated cellular responses that would be expected from treatment with Bone Morphogenic Proteins (BMP’s) and Transforming Growth Factor- beta (TGF-β) [163]. The biochemical responses of bone marrow stromal cells (hBMSC) to nacre for instance are like-for-like following treatment with nanogram quantities of TGF-β.…”

Section: Biomedical Applications Of Marine Productsmentioning

confidence: 99%

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Evolving Marine Biomimetics for Regenerative Dentistry

2014

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New products that help make human tissue and organ regeneration more effective are in high demand and include materials, structures and substrates that drive cell-to-tissue transformations, orchestrate anatomical assembly and tissue integration with biology. Marine organisms are exemplary bioresources that have extensive possibilities in supporting and facilitating development of human tissue substitutes. Such organisms represent a deep and diverse reserve of materials, substrates and structures that can facilitate tissue reconstruction within lab-based cultures. The reason is that they possess sophisticated structures, architectures and biomaterial designs that are still difficult to replicate using synthetic processes, so far. These products offer tantalizing pre-made options that are versatile, adaptable and have many functions for current tissue engineers seeking fresh solutions to the deficiencies in existing dental biomaterials, which lack the intrinsic elements of biofunctioning, structural and mechanical design to regenerate anatomically correct dental tissues both in the culture dish and in vivo.

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“…Pearl oyster nacre is able to induce osteogenesis and bone formation from latent osteoprogenitors along an endochondral pathway, consisting of a cartilage tissue intermediary phase (Lamghari et al, 2001a; 2001b; Lopez et al, 1992; 2004a; 2004b; Milet et al, 2004; Shen et al, 2006; Silve et al, 1992). Nacre has been tested in human, sheep and rabbit models (Lamghari et al, 1999; 2001b; Liao et al, 2002). In human patients with critical size, non-union bone defects, fresh woven bone welded itself throughout the nacre implant with participation of osteoclasts and osteoblasts.…”

Section: Introductionmentioning

confidence: 99%

Osteogenic Potency of Nacre on Human Mesenchymal Stem Cells

Green

Kwon²,

Jung

2015

Molecules and Cells

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Nacre seashell is a natural osteoinductive biomaterial with strong effects on osteoprogenitors, osteoblasts, and osteoclasts during bone tissue formation and morphogenesis. Although nacre has shown, in one study, to induce bridging of new bone across large non-union bone defects in 8 individual human patients, there have been no succeeding human surgical studies to confirm this outstanding potency. But the molecular mechanisms associated with nacre osteoinduction and the influence on bone marrow-derived mesenchymal stem cells (BMSC’s), skeletal stem cells or bone marrow stromal cells remain elusive. In this study we highlight the phenotypic and biochemical effects of Pinctada maxima nacre chips and the global nacre soluble protein matrix (SPM) on primary human bone marrow-derived stromal cells (hBMSCs) in vitro. In static co-culture with nacre chips, the hBMSCs secreted Alkaline phosphatase (ALP) at levels that exceeded bone morphogenetic protein (rhBMP-2) treatment. Concentrated preparation of SPM applied to Stro-1 selected hBMSC’s led to rapid ALP secretions, at concentrations exceeding the untreated controls even in osteogenic conditions. Within 21 days the same population of Stro-1 selected hBMSCs proliferated and secreted collagens I–IV, indicating the premature onset of an osteoblast phenotype. The same SPM was found to promote unselected hBMSC differentiation with osteocalcin detected at 7 days, and proliferation increased at 7 days in a dose-dependent manner. In conclusion, nacre particles and nacre SPM induced the early stages of human bone cell differentiation, indicating that they may be promising soluble factors with osteoinductive capacity in primary human bone cell progenitors such as, hBMSC’s.

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Stimulation of bone marrow cells and bone formation by nacre: in vivo and in vitro studies

Cited by 122 publications

References 9 publications

Water Soluble Bioactives of Nacre Mediate Antioxidant Activity and Osteoblast Differentiation

Water Soluble Bioactives of Nacre Mediate Antioxidant Activity and Osteoblast Differentiation

Evolving Marine Biomimetics for Regenerative Dentistry

Osteogenic Potency of Nacre on Human Mesenchymal Stem Cells

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