Stimulation of biofilm formation by insertion of Tetrahymena pyriformis wells within Burkholderia cenocepacia biofilms

Kaminskaya, Anastasya A.; Pushkareva, V. I.; Moisenovich, Mikhail M.; Степанова, Т. В.; Волкова, Н. В.; Romanova, Julia M.; Litvin, V. Yu.; Гинцбург, А Л; Ermolaeva, S. A.

doi:10.3103/s0891416807040088

Cited by 7 publications

(4 citation statements)

References 27 publications

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“…The monospecies grazing experiments thus indicate differential bacterial behavior in response to a predator and vice-versa. Similar observations have been reported previously where protozoa regulate the social behavior of the bacteria ( Rønn et al, 2002 ; Scherwass et al, 2016 ) or where bacteria regulate the protozoan population ( Kaminskaya et al, 2007 ). The specificity of such responses has been reported to vary depending on the selected bacteria and protozoa ( Dopheide et al, 2011 ; Friman et al, 2013 ).…”

Section: Discussionsupporting

confidence: 89%

“…Grazing by protozoa has been reported to stimulate micro-colony formation, alter mass transfer of nutrients and induce biofilm development by stimulating bacterial layer thickness ( Matz et al, 2004 ; Weitere et al, 2005 ; Kaminskaya et al, 2007 ; Wey et al, 2008 ; Böhme et al, 2009 ). Other studies, however, argue that protozoa do not induce biofilm formation ( Huws et al, 2005 ) but instead show a marked preference for grazing on attached or aggregated bacterial cells or only change biofilm community structure ( Caron, 1987 ; Sibbald and Albright, 1988 ; Huws et al, 2005 ; Wey et al, 2008 ).…”

Section: Introductionmentioning

confidence: 99%

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Synergistic Interactions within a Multispecies Biofilm Enhance Individual Species Protection against Grazing by a Pelagic Protozoan

et al. 2018

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Biofilm formation has been shown to confer protection against grazing, but little information is available on the effect of grazing on biofilm formation and protection in multispecies consortia. With most biofilms in nature being composed of multiple bacterial species, the interactions and dynamics of a multispecies bacterial biofilm subject to grazing by a pelagic protozoan predator were investigated. To this end, a mono and multispecies biofilms of four bacterial soil isolates, namely Xanthomonas retroflexus, Stenotrophomonas rhizophila, Microbacterium oxydans and Paenibacillus amylolyticus, were constructed and subjected to grazing by the ciliate Tetrahymena pyriformis. In monocultures, grazing strongly reduced planktonic cell numbers in P. amylolyticus and S. rhizophila and also X. retroflexus. At the same time, cell numbers in the underlying biofilms increased in S. rhizophila and X. retroflexus, but not in P. amylolyticus. This may be due to the fact that while grazing enhanced biofilm formation in the former two species, no biofilm was formed by P. amylolyticus in monoculture, either with or without grazing. In four-species biofilms, biofilm formation was higher than in the best monoculture, a strong biodiversity effect that was even more pronounced in the presence of grazing. While cell numbers of X. retroflexus, S. rhizophila, and P. amylolyticus in the planktonic fraction were greatly reduced in the presence of grazers, cell numbers of all three species strongly increased in the biofilm. Our results show that synergistic interactions between the four-species were important to induce biofilm formation, and suggest that bacterial members that produce more biofilm when exposed to the grazer not only protect themselves but also supported other members which are sensitive to grazing, thereby providing a “shared grazing protection” within the four-species biofilm model. Hence, complex interactions shape the dynamics of the biofilm and enhance overall community fitness under stressful conditions such as grazing. These emerging inter- and intra-species interactions could play a vital role in biofilm dynamics in natural environments like soil or aquatic systems.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Synergistic Interactions within a Multispecies Biofilm Enhance Individual Species Protection against Grazing by a Pelagic Protozoan

et al. 2018

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“…The Gram-negative bacteria B. cenocepacia strain Bc 46 and P. aeruginosa strain PA103 were used to grow biofilms. In general, the bacteria were grown in biofilms as described previously (Kaminskaya et al, 2007). Briefly, an overnight culture was diluted 1000-fold in Luria-Bertani broth and cultured without agitation at 28 uC for 72 h. A coverglass of 1.5 cm 2 was placed vertically into the flask to allow a biofilm to form on its surface.…”

Section: Methodsmentioning

confidence: 99%

“…Thereafter, the biofilms were labelled using a Live/Dead Cell Viability Assay kit (Invitrogen) according to the manufacturer's instructions. Digital images were acquired with a confocal laser-scanning microscope (Zeiss Axiovert 200M LSM 510 META) as described by Kaminskaya et al (2007).…”

Section: Methodsmentioning

confidence: 99%

Bactericidal effects of non-thermal argon plasma in vitro, in biofilms and in the animal model of infected wounds

Sа

Varfolomeev

Chernukha

et al. 2011

Journal of Medical Microbiology

298

167

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Non-thermal (low-temperature) physical plasma is under intensive study as an alternative approach to control superficial wound and skin infections when the effectiveness of chemical agents is weak due to natural pathogen or biofilm resistance. The purpose of this study was to test the individual susceptibility of pathogenic bacteria to non-thermal argon plasma and to measure the effectiveness of plasma treatments against bacteria in biofilms and on wound surfaces. Overall, Gram-negative bacteria were more susceptible to plasma treatment than Gram-positive bacteria. For the Gramnegative bacteria Pseudomonas aeruginosa, Burkholderia cenocepacia and Escherichia coli, there were no survivors among the initial 10 5 c.f.u. after a 5 min plasma treatment. The susceptibility of Gram-positive bacteria was species-and strain-specific. Streptococcus pyogenes was the most resistant with 17 % survival of the initial 10 5 c.f.u. after a 5 min plasma treatment. Staphylococcus aureus had a strain-dependent resistance with 0 and 10 % survival from 10 5 c.f.u. of the Sa 78 and ATCC 6538 strains, respectively. Staphylococcus epidermidis and Enterococcus faecium had medium resistance. Non-ionized argon gas was not bactericidal. Biofilms partly protected bacteria, with the efficiency of protection dependent on biofilm thickness. Bacteria in deeper biofilm layers survived better after the plasma treatment. A rat model of a superficial slash wound infected with P. aeruginosa and the plasma-sensitive Staphylococcus aureus strain Sa 78 was used to assess the efficiency of argon plasma treatment. A 10 min treatment significantly reduced bacterial loads on the wound surface. A 5-day course of daily plasma treatments eliminated P. aeruginosa from the plasma-treated animals 2 days earlier than from the control ones. A statistically significant increase in the rate of wound closure was observed in plasma-treated animals after the third day of the course. Wound healing in plasma-treated animals slowed down after the course had been completed. Overall, the results show considerable potential for non-thermal argon plasma in eliminating pathogenic bacteria from biofilms and wound surfaces.Abbreviation: CI, confidence interval.

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Low Temperature Atmospheric Argon Plasma: Diagnostics and Medical Applications

Sа

Петров

Zigangirova

et al. 2011

Plasma for Bio-Decontamination, Medicine and Food Security

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Stimulation of biofilm formation by insertion of Tetrahymena pyriformis wells within Burkholderia cenocepacia biofilms

Cited by 7 publications

References 27 publications

Synergistic Interactions within a Multispecies Biofilm Enhance Individual Species Protection against Grazing by a Pelagic Protozoan

Synergistic Interactions within a Multispecies Biofilm Enhance Individual Species Protection against Grazing by a Pelagic Protozoan

Bactericidal effects of non-thermal argon plasma in vitro, in biofilms and in the animal model of infected wounds

Low Temperature Atmospheric Argon Plasma: Diagnostics and Medical Applications

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