No abstract

An experimental determination of particle charge in a bulk dc discharge plasma covering a wide range of neutral gas pressures, was recently reported [S. Ratynskaia, Phys. Rev. Lett. 93, 085001 (2004)]. The charges obtained were several times smaller than the predictions of collisionless orbital motion limited theory. This discrepancy was attributed to the effect of ion-neutral collisions. In the present paper a more detailed description of this experiment is provided and additional experimental results obtained with particles of different sizes are reported. The measurements are compared with molecular dynamics simulations of particle charging for conditions similar to those of the experiment, with other available experimental data on particle charge in the bulk of gas discharges, and with a simple analytical model accounting for ion-neutral collisions. All the considered evidence indicates that ion-neutral collisions represent a very important factor, which significantly affects (reduces) the particle charge under typical discharge conditions.

No abstract

Non-thermal (low-temperature) physical plasma is under intensive study as an alternative approach to control superficial wound and skin infections when the effectiveness of chemical agents is weak due to natural pathogen or biofilm resistance. The purpose of this study was to test the individual susceptibility of pathogenic bacteria to non-thermal argon plasma and to measure the effectiveness of plasma treatments against bacteria in biofilms and on wound surfaces. Overall, Gram-negative bacteria were more susceptible to plasma treatment than Gram-positive bacteria. For the Gramnegative bacteria Pseudomonas aeruginosa, Burkholderia cenocepacia and Escherichia coli, there were no survivors among the initial 10 5 c.f.u. after a 5 min plasma treatment. The susceptibility of Gram-positive bacteria was species-and strain-specific. Streptococcus pyogenes was the most resistant with 17 % survival of the initial 10 5 c.f.u. after a 5 min plasma treatment. Staphylococcus aureus had a strain-dependent resistance with 0 and 10 % survival from 10 5 c.f.u. of the Sa 78 and ATCC 6538 strains, respectively. Staphylococcus epidermidis and Enterococcus faecium had medium resistance. Non-ionized argon gas was not bactericidal. Biofilms partly protected bacteria, with the efficiency of protection dependent on biofilm thickness. Bacteria in deeper biofilm layers survived better after the plasma treatment. A rat model of a superficial slash wound infected with P. aeruginosa and the plasma-sensitive Staphylococcus aureus strain Sa 78 was used to assess the efficiency of argon plasma treatment. A 10 min treatment significantly reduced bacterial loads on the wound surface. A 5-day course of daily plasma treatments eliminated P. aeruginosa from the plasma-treated animals 2 days earlier than from the control ones. A statistically significant increase in the rate of wound closure was observed in plasma-treated animals after the third day of the course. Wound healing in plasma-treated animals slowed down after the course had been completed. Overall, the results show considerable potential for non-thermal argon plasma in eliminating pathogenic bacteria from biofilms and wound surfaces.Abbreviation: CI, confidence interval.

No abstract

The charge of dust particles is determined experimentally in a bulk dc discharge plasma in the pressure range 20-100 Pa. The charge is obtained by two independent methods: one based on an analysis of the particle motion in a stable particle flow and another on an analysis of the transition of the flow to an unstable regime. Molecular-dynamics simulations of the particle charging for conditions similar to those of the experiment are also performed. The results of both experimental methods and the simulations demonstrate good agreement. The charge obtained is several times smaller than predicted by the collisionless orbital motion theory, and thus the results serve as an experimental indication that ion-neutral collisions significantly affect particle charging.

An observation of low frequency waves spontaneously excited in a dc glow discharge dusty plasma is reported. To analyze possible reasons for the instability observed, a linear dispersion relation which takes into account collisions with neutrals, dust grain charge variations, ion drift, and forces acting on dust particles is derived. Numerical analysis of the dispersion relation shows that the observed instability is the result of dust charge variations in the presence of external charge-dependent forces together with the ion drift effect.

The results of numerical study of mass-transfer processes in quasi-two- and three-dimensional nonideal dissipative systems are presented. Simulations were performed for different types of model pair potentials of intergrain interaction that are various combinations of power-law and exponential functions. The calculations were performed in a wide range of parameters typical for laboratory dusty plasma experiments. It was shown that the dynamics of grains in liquidlike systems for short observation times is close to the evolution of thermal oscillations in the crystal lattice.