Stimulation of Antibody-Forming Cells by Oligonucleotides of Known Composition

Braun, Werner; Nakano, Masayasu; Jaraskova, Lydie; Yajima, Y; Jiménez, Luis Pablo Calle

doi:10.1007/978-3-642-87668-4_23

Cited by 29 publications

(12 citation statements)

References 26 publications

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“…4), phagocytosis (41), and production of specific antibody (38)(39)(40) support the hypothesis that a common mechanism (release of oligonucleotides ? [43]) might underlie interferon production, antiviral protection, and the other host resistance mechanisms.…”

Section: Resultsmentioning

confidence: 79%

“…The plant cytokinines, kinetin riboside and isopentenyladenosine, reverse the stimulatory effects of oligodeoxyribonucleotides and double-stranded RNA's such as (poly rA)-(poly rU) on antibody production (38)(39)(40) and phagocytosis (41); kinetin riboside also antagonizes the effect of endotoxin on host resistance to infection with Pseudomonas aeruginosa (15). Kinetin riboside and isopentenyladenosine do not inhibit the normal immune response, but only the adjuvant-like effects on antibody production.…”

Section: Resultsmentioning

confidence: 99%

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The role of interferon in the protective effect of a synthetic double-stranded polyribonucleotide against intranasal vesicular stomatitis virus challenge in mice

Clerco¹,

Nuwer²,

Merigan³

1970

J. Clin. Invest.

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A B S T R A C T Intravenous injection of polyinosinic acid/polycytidylic acid [(poly rI) * (poly rC)] offered significant protection against intranasal challenge of young mice with vesicular stomatitis virus (VSV). Optimal protection was obtained when a single dose was administered 2 hr before virus challenge, but repeated doses were effective when started as late as 3 days after virus challenge. The therapeutic ratio or ratio of maximum tolerated dose to minimum effective dose for a single intravenous injection of (poly rI) *(poly rC) 2 hr before virus inoculation was > 8 mg/kg: 0.004 mg/ kg or > 200. Dose-response curves for interferon production and antiviral protection by (poly rI) -(poly rC) were closely parallel. Equivalent doses of poly rI or poly rC alone did not exert any interferon-inducing capacity or protective effect on intranasal VSV challenge. Several factors, which are known to potentiate or antagonize interferon production, increased or decreased the interferon-inducing capacity and antiviral protection of either (poly rI) * (poly rC) or maleic acid/divinyl ether copolymer (MA/DVE) in parallel. Interferon production and antiviral protection by MA/DVE were enhanced by arginine but abolished by prior treatment with MA/DVE; DEAE-dextran (intraperitoneally), kinetin riboside and isopentenyladenosine, and prior injection of endotoxin reduced both interferon production and antiviral protection by (poly rI) -(poly rC).Treatment with exogenous interferon in amounts which closely mimicked the levels of circulating interferon produced endogenously by an effective dose of (poly rI) * (poly rC) gave protection against intranasal

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Section: Resultsmentioning

confidence: 79%

Section: Resultsmentioning

confidence: 99%

The role of interferon in the protective effect of a synthetic double-stranded polyribonucleotide against intranasal vesicular stomatitis virus challenge in mice

Clerco¹,

Nuwer²,

Merigan³

1970

J. Clin. Invest.

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“…Braun et al [5] reported that poly A:U (a polyanion of phosphated polymer type) enhances secondary response less effectively than primary immune response t o SRBC in mice. Moreover, the effect of poly A:U on secondary response was restricted t o 19 S antibody formation, while their effect o n 7 S PFC was reported t o be of "questionable significance".…”

Section: Discussionmentioning

confidence: 99%

“…The molecular weight of the polyanions does not seem to be critical in the range of 2 x l o 3 -5 x lo'. The aim of the present report was to investigate the effects of dextran sulfate on the kinetics of the primary and secondary response and to show whether the enhancement of the secondary response by this polyanion is limited t o the 19 S antibody formation as reported for polynucleotides [ 5 ] .…”

Section: Introductionmentioning

confidence: 92%

Stimulation of humoral antibody formation by polyanions. IV. The effects of dextran sulfate on the kinetics of secondary immune response in mice

et al. 1971

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sulfate enhances secondary immune response as well as primary immune response in mice primed and challenged with suboptimal doses of sheep red blood cells. Enhancement of primary response by dextran sulfate did not diminish secondary responses towards suboptimal antigen dose. However, in mice primed and challenged with an optimal antigen dose and injected at the time of priming with dextran sulfate, immunological memory seems t o be increased. In primary as well as in secondary response the number of both 19 S and 7 S PFC were increased.

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“…However, no increase in ro settes occurs when bone marrow cells are similarly treated with the po lynucleotide complexes [2]. These results indicate that the adjuvant ac tion of poly A:U [3,4] in the primary immune response is mediated in part through stimulation of thymus-derived (T) lymphocytes.…”

Section: Introductionmentioning

confidence: 87%

Adjuvant Action of Poly A:U on T and B Rosette-Forming Cells in SRBC-Immunized Mice

Cone

Wilson

1972

Int Arch Allergy Immunol

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The adjuvant action of complexes of polyadenylic and polyuridylic acids (poly A:U) on the primary immune response to SRBC was studied with respect to its effect on the production of thymus-derived (T) and bone marrow-derived (B) rosette-forming cells (RFC). RFC were assayed by two separate methods; one in which rosettes were prepared by incubation at 37 °C and then at 4 °C, the other by centrifugation at 4 °C. Two to three times more RFC were detected in mice receiving SRBC only when RFC were assayed by the incubation method. Most RFC detected by the incubation method were Θ-negative (BRFC) whereas 15–32% of the RFC detected by the centrifugation method were Θ -positive (TRFC). Both methods detected a 2- to 3-fold amplification of BRFC in mice receiving SRBC plus poly A:U. However, while the centrifugation method detected a 2- to 3-fold increase in T RFC, the incubation method detected a greater than 10-fold amplification of T RFC.

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Stimulation of Antibody-Forming Cells by Oligonucleotides of Known Composition

Cited by 29 publications

References 26 publications

The role of interferon in the protective effect of a synthetic double-stranded polyribonucleotide against intranasal vesicular stomatitis virus challenge in mice

The role of interferon in the protective effect of a synthetic double-stranded polyribonucleotide against intranasal vesicular stomatitis virus challenge in mice

Stimulation of humoral antibody formation by polyanions. IV. The effects of dextran sulfate on the kinetics of secondary immune response in mice

Adjuvant Action of Poly A:U on T and B Rosette-Forming Cells in SRBC-Immunized Mice

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