Stimulation of 2',5'-oligoadenylate synthetase activity in sheep endometrium during pregnancy, by intrauterine infusion of ovine trophoblast protein-1, and by intramuscular administration of recombinant bovine interferon- I1

Mirando, Mark A.; Short, Everett C.; Geisert, R. D.; Vallet, J. L.; Bazer, Fuller W.

doi:10.1530/jrf.0.0930599

Cited by 38 publications

(19 citation statements)

References 28 publications

(37 reference statements)

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“…In addition to its antiluteolytic actions, IFNT increases expression of several IFNstimulated genes (ISGs) that are hypothesized to be important for endometrial differentiation and implantation of the conceptus (Hansen et al 1999, Bazer et al 2010. These ISGs include STAT1 and STAT2 (Johnson et al 1999a, 1999d, Stewart et al 2001a, IFN regulatory factor-1 (IRF1; Spencer et al 1998, Johnson et al 1999a, 1999d, Stewart et al 2001a, IRF9 (Stewart et al 2002), ISG15 (Johnson et al 1999a, 1999b, 1999d, Stewart et al 2001a, MX , OAS (Mirando et al 1991, MIC , B2M ,…”

Section: Introductionmentioning

confidence: 99%

Pregnancy and interferon τ regulate DDX58 and PLSCR1 in the ovine uterus during the peri-implantation period

et al. 2011

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Interferon t (IFNT), the pregnancy recognition signal in ruminants, abrogates the luteolytic mechanism for maintenance of the corpus luteum for production of progesterone (P 4 ). This study examined the expression of DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58) and phospholipid scramblase 1 (PLSCR1) mRNAs in the ovine uterus as these genes were increased most in 2fTGH (STAT1 positive) cells by IFNT. The results of this study indicated that IFNT regulates expression of DDX58 and PLSCR1 mRNAs in the ovine uterus, which confirmed the results of the in vitro transcriptional profiling experiment with the 2fTGH (parental STAT1 positive) and U3A (STAT1 null) cell lines. Steady-state levels of DDX58 and PLSCR1 mRNAs increased in cells of the ovine uterus between days 12 and 20 of pregnancy, but not between days 10 and 16 of the estrous cycle. The expression of DDX58 and PLSCR1 mRNAs was greatest in endometrial stromal cells, but there was transient expression in uterine luminal and superficial glandular epithelial cells. P 4 alone did not induce expression of DDX58 and PLSCR1 mRNAs; however, intrauterine injections of IFNT did induce expression of DDX58 and PLSCR1 mRNAs in the endometria of nonpregnant ewes independent of effects of P 4 . These results indicate that IFNT induces expression of DDX58 and PLSCR1 in ovine endometrial cells via the classical STAT1-mediated cell signaling pathway. Based on their known biological effects, DDX58 and PLSCR1 are IFN-stimulated genes, which may increase the antiviral status of cells of the pregnant uterus to protect against viral infection and/or enhance secretion of type I IFNs that inhibit viral replication.

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Section: Introductionmentioning

confidence: 99%

Pregnancy and interferon τ regulate DDX58 and PLSCR1 in the ovine uterus during the peri-implantation period

et al. 2011

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“…The IFN induces or increases expression of many IFN-stimulated genes (ISGs) in a celltype specific manner in the uterine endometrium. These ISGs include ␤2MG [23], Stat1 and Stat2 [24][25][26][27][28], IRF-1 [24,25,27,28], ISG17 [25,[29][30][31], Mx protein [32,33], and 2Ј,5Ј-oligoadenylate synthetase (OAS) [34,35]. The IFN regulates gene transcription through an intracellular signal transduction system involving Stats and IRFs [25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Pregnancy and Interferon Tau Regulate Major Histocompatibility Complex Class I and β2-Microglobulin Expression in the Ovine Uterus1

Choi¹,

Johnson

Spencer³

et al. 2003

Biology of Reproduction

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Major histocompatibility complex (MHC) class I molecules, consisting of an alpha chain and beta2-microglobulin (beta2MG), play an important role in immune rejection responses by discriminating self and nonself and are increased by type I interferons during antiviral responses. Interferon tau (IFNtau), the pregnancy-recognition signal in ruminants, is a type I interferon produced by the ovine conceptus between Days 11 and 21 of gestation. In study 1, expression of MHC class I alpha chain and beta2MG mRNA and protein was detected primarily in endometrial luminal epithelium (LE) and glandular epithelium (GE) on Days 10 and 12 of the estrous cycle and pregnancy. On Days 14-20 of pregnancy, MHC class I and beta2MG expression increased only in endometrial stroma and GE and, concurrently, was absent in LE and superficial ductal GE (sGE). Although neither MHC class I nor beta2MG proteins were detected in Day 20 trophectoderm, beta2MG mRNA was detected in conceptus trophectoderm. In study 2, cyclic ewes were ovariectomized on Day 5, treated daily with progesterone to Day 16, received intrauterine infusions between Days 11 and 16 of either control serum proteins or recombinant ovine IFNtau, and were hysterectomized on Day 17. The IFNtau increased MHC class I and beta2MG expression only in endometrial stroma and GE. During pregnancy, MHC class I and beta2MG gene expression is inhibited in endometrial LE and sGE but, paradoxically, is stimulated by IFNtau in the stroma and GE. The silencing of MHC class I alpha chain and beta2MG genes in the endometrial LE and sGE during pregnancy recognition and establishment may be a critical mechanism preventing immune rejection of the conceptus allograft.

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“…In sheep, IFN binds to classical type I IFN receptors on the endometrial luminal (LE) and superficial glandular epithelia (GE) to prevent uterine release of luteolytic pulses of prostaglandin F 2␣ (PGF) by suppressing transcription of epithelial estrogenreceptor alpha (ER␣) and oxytocin receptor (OTR) genes [1,4]. IFN induces or increases expression of a number of IFN-stimulated genes (ISGs) in the ruminant endometrium or endometrial cell lines, including signal transducer and activator of transcription (STAT) 1 and 2 [5,6], ␤ 2 -microglobulin [7], IFN regulatory factor one (IRF-1) [5,6,8], ISG17/ubiquitin cross-reactive protein (ISG17/UCRP) [5,6,9], Mx protein [10], and 2Ј,5Ј oligoadenylate synthetase (OAS) [11]. Recent evidence indicates that effects of IFN on endometrial gene expression are mediated by an intracellular signal transduction system involving STAT1, STAT2, and IRFs [5,6,8].…”

Section: Introductionmentioning

confidence: 99%

Effects of the Estrous Cycle, Pregnancy, and Interferon Tau on 2′,5′-Oligoadenylate Synthetase Expression in the Ovine Uterus1

Johnson

Stewart

Gray

et al. 2001

Biology of Reproduction

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The enzymes which comprise the 2',5'-oligoadenylate synthetase (OAS) family are interferon (IFN) stimulated genes which regulate ribonuclease L antiviral responses and may play additional roles in control of cellular growth and differentiation. This study characterized OAS expression in the endometrium of cyclic and pregnant ewes as well as determined effects of IFNtau and progesterone on OAS expression in cyclic or ovariectomized ewes and in endometrial epithelial and stromal cell lines. In cyclic ewes, low levels of OAS protein were detected in the endometrial stroma (S) and glandular epithelium (GE). In early pregnant ewes, OAS expression increased in the S and GE on Day 15. OAS expression in the lumenal epithelium (LE) was not detected in uteri from either cyclic or pregnant ewes. Intrauterine administration of IFNtau stimulated OAS expression in the S and GE, and this effect of IFNtau was dependent on progesterone. Ovine endometrial LE, GE, and S cell lines responded to IFNtau with induction of OAS proteins. In all three cell lines, the 40/46-kDa OAS forms were induced by IFNtau, whereas the 100-kDa OAS form appeared to be constitutively expressed and not affected by IFNtau. The 69/71-kDa OAS forms were induced by IFNtau in the S and GE cell lines, but not in the LE. Collectively, these results indicate that OAS expression in the endometrial S and GE of the early pregnant ovine uterus is directly regulated by IFNtau from conceptus and requires the presence of progesterone.

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Stimulation of 2',5'-oligoadenylate synthetase activity in sheep endometrium during pregnancy, by intrauterine infusion of ovine trophoblast protein-1, and by intramuscular administration of recombinant bovine interferon- I1

Cited by 38 publications

References 28 publications

Pregnancy and interferon τ regulate DDX58 and PLSCR1 in the ovine uterus during the peri-implantation period

Pregnancy and interferon τ regulate DDX58 and PLSCR1 in the ovine uterus during the peri-implantation period

Pregnancy and Interferon Tau Regulate Major Histocompatibility Complex Class I and β2-Microglobulin Expression in the Ovine Uterus1

Effects of the Estrous Cycle, Pregnancy, and Interferon Tau on 2′,5′-Oligoadenylate Synthetase Expression in the Ovine Uterus1

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