Stimulation by Ovine Prolactin of Fluid Transfer in Everted Sacs of Rat Small Intestine

Ramsey, D. H.; Bern, H. A.

doi:10.1677/joe.0.0530453

Cited by 39 publications

(24 citation statements)

References 14 publications

(20 reference statements)

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“…PRL, which is known to affect ion transport across epithelial cells, could act at the apical membrane of mammary epithelial cells or on intestinal cells of the suckling pups. It has been recently shown that PRL could be transported across the epithelium of thejejunum and ileum of the suckling rat (18) and it is documented that PRL may affect osmoregulation in the intestine (19). GH receptors have also been identified by immunochemistry in different parts of the intestine of the rat (20).…”

Section: Discussionmentioning

confidence: 99%

Identification of prolactin and growth hormone binding proteins in rabbit milk.

Postel-Vinay

Belair

Kayser

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

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Two distinct soluble proteins that specifically bind 125I-labeled human growth hormone (GH) are identified in the supernatant of ultracentrifuged rabbit milk, using HPLC gel filtration. The higher molecular weight protein is GH specific, whereas the other one is specific for prolactin (PRL). 125I-labeled human GH, the M, of the GH-binding protein and of the PRL-binding protein were estimated to be 51,000 and 33,000, respectively. The binding proteins identified in the present work are probably responsible for the transport oftheir specific ligands in the milk. It is also conceivable that they have a role in the effects of GH and PRL in the mammary gland and/or the intestine of the young.

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Section: Discussionmentioning

confidence: 99%

Identification of prolactin and growth hormone binding proteins in rabbit milk.

Postel-Vinay

Belair

Kayser

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

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“…It is clear that there are unidentified dietary constituents) that selectively influence the jejunal mechanism for the active transfer of ac-methyl glucoside. Different commercial diets are known to alter the sensitivity of intestine to hormonal actions (Ramsey & Bern, 1972) and influence the levels of various enzymes involved in digestion and transport (Heitanen, 1975).…”

Section: Discussionmentioning

confidence: 99%

Effects of fasting and semistarvation on the kinetics of active and passive sugar absorption across the small intestine in vivo.

Debnam¹,

Levin²

1975

The Journal of Physiology

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SUMMARY1. The effects of dietary restriction on the kinetics of absorption in vivo of glucose, galactose and a-methyl glucoside were assessed by electrical and chemical methods in the rat jejunum.2. The 'apparent Ki', maximum absorption or Vmax (jc-mole/ 1O cm.15 min) and maximum potential difference (p.d.max) were obtained for the jejunal electrogenic active transfer mechanism from the transfer p.d.s and the chemical absorption data corrected for diffusion using various graphical kinetic plots.3. Fasting for 3 days greatly decreased the 'apparent K.s', obtained from electrical or chemical data, for all the sugars but had no effect on those for L-valine or L-methionine. Semistarvation caused a less pronounced reduction of the 'apparent Kms' for the sugars. The dietaryinduced change in 'apparent K.' for glucose was also observed in the fasted hamster. One interpretation of these changes is that the affinity of the carriers for sugars increases during dietary restriction; the greater the level of restriction the greater the increase.4. Fasting and semistarvation caused large reductions in the Vmax. These reductions were correlated with a reduced enterocyte population estimated by changes in enterocyte column size.5. The reduction in the VmaX for galactose was mainly accounted for by the decrease in enterocyte population. In the case of glucose, other factors such as reduced enterocyte metabolism or changes in the carriers must be involved to explain the discrepancy between the large decrease in Vmax and the enterocyte column size.6. Fasting and semi-starvation had complex, differential actions on the * Present address: Department of Physiology, Royal Free Hospital School of Medicine, Hunter Street, London, WC1N IBP. E. S. DEBNAM AND R. J. LEVIN p.d.max for glucose, galactose and x-methyl glucoside. These changes did not correlate with those observed in the Vmax measured chemically. 7. A standard diet obtained from two commercial sources was found to differ greatly in its effect on the electrogenic transfer system for cx-methyl glucoside but had no effect on those for galactose and glucose.

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“…Adult female Sprague-Dawley rats weighing between 200 to 250 g sewed as controls. In a group of the same species, 1 mg of ovine prolactin (31 IU/mg; Sigma Chemical Co., St. Louis, MO) was injected subcutaneously, 24 and 48 h before the intestine was removed for incubation (15). Prolactin was dissolved in TS hormone solvent (Sigma) containing 3 mg phenol and 50 mg glucose per ml water.…”

Section: Methodsmentioning

confidence: 99%