Food Proteins and Gut Mucosal Barrier. III. The Influence of Lactation and Prolactin on the in Vitro Binding and Uptake of Bovine Serum Albumin and Ovalbumin in the Rat Jejunum

Stern, Martin; Harmatz, Paul; Kleinman, Ronald E.; Walker, W. Allan

doi:10.1203/00006450-198503000-00014

Cited by 5 publications

(2 citation statements)

References 12 publications

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“…Our results confirm earlier studies (6,21,22) as far as intestinal binding and uptake of BSA are concerned. Binding and uptake curves for the cow's milk proteins/peptides tested were running nearly in parallel.…”

Section: Discussionsupporting

confidence: 92%

Handling of Gliadin Peptides B1‐B4 and of Cow's Milk Proteins by Rat Jejunum Gut Sacs

Melter,

Belitz,

Gellermann

et al. 1988

J. pediatr. gastroenterol. nutr.

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SummaryThe rat everted gut sac was used as a model of intestinal protein and peptide handling to study small intestinal binding and uptake of different gliadin peptide fractions (B1, B2, B3, B4) in comparison with bovine serum albumin (BSA), α‐lactalbumin (α‐LA), and a BSA peptide fraction (BSA‐P). Not only binding curves, but also uptake curves of BSA, α‐LA, and BSA‐P were run in parallel between 1 and 30 min. Binding and uptake obviously depended on molecular weight. BSA‐P binding was found to be highest of all. Binding of BSA was significantly lower. After 20 and 30 min, significantly more α‐LA and BSA‐P were taken up than was BSA. There was no difference in handling of the gliadin peptide fractions tested by the gut mucosal surface. Binding of B1‐B4 was significantly higher than binding of BSA and was significantly lower than binding of BSA‐P. No difference was found between binding of B1‐B4 and a‐LA. There was a consistently higher uptake of all gliadin peptide fractions, independent of molecular weight. Slopes for uptake curves of B1‐B4 were steeper than those of BSA, α‐LA, and BSA‐P. Crossing of uptake over binding curves was consistently observed with gliadin fractions, but never with cow's milk proteins/peptides. Differences in uptake between gliadin peptide fractions and cow's milk proteins/peptides might be based on a specific interaction between gliadin peptides and the gut surface.

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Section: Discussionsupporting

confidence: 92%

Handling of Gliadin Peptides B1‐B4 and of Cow's Milk Proteins by Rat Jejunum Gut Sacs

Melter,

Belitz,

Gellermann

et al. 1988

J. pediatr. gastroenterol. nutr.

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“…11], maturation and growth of the gut [12], and 1,25-OH vitamin D production [13]. Steadily increasing fetal serum prolactin levels [14] and the prolonged hyperprolactinemia of the preterm infant [15] are among the findings that support the role of prolactin during human development.…”

Section: Introductionmentioning

confidence: 91%

Developmental Regulation of the Inhibitory Effect of Dopamine on Prolactin Release in the Preterm Neonate

Seri

Somogyvari²,

Hovanyovszky³

et al. 1998

Neonatology

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The secretion and release of prolactin from the anterior pituitary is under the tonic inhibitory control of endogenous dopamine produced in the central nervous system. Exogenous dopamine inhibits prolactin secretion by reaching the pituitary via the portal circulation, and the hypolactotropic effect of dopamine infusion has been documented in all age groups in humans. However, the maturation of lactotroph sensitivity to dopaminergic inhibition has not been studied. Therefore, we followed the changes in serum prolactin concentrations before, during, and after dopamine infusion in 19 sick preterm infants with a mean gestational age of 30.6 ± 0.6 weeks during the first 3 days of life, and examined the relationship of the hypolactotropic effect of dopamine to gestational age and birth weight in this patient population. As expected, dopamine therapy resulted in a decrease in mean serum prolactin from 89.4 ± 9.5 to 58.6 ± 9.1 µg/l (p < 0.05) with a return of the serum prolactin concentration to the pretreatment level 2–6 h after discontinuation of drug administration (98.3 ± 11.7 µg/l, p < 0.05). However, simple regression analysis of the individual data revealed that the magnitude of the dopamine-induced decrease in serum prolactin was significantly influenced by gestational age (p = 0.006) and birthweight (p = 0.037). Thus, our findings provide evidence for the maturation of pituitary lactotroph sensitivity to dopaminergic inhibition in the preterm human neonate.

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Uptake of polypeptide fragments of proteins by rat intestine in vitro and in vivo

et al. 1988

Gastroenterology

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Food Proteins and Gut Mucosal Barrier. III. The Influence of Lactation and Prolactin on the in Vitro Binding and Uptake of Bovine Serum Albumin and Ovalbumin in the Rat Jejunum

Cited by 5 publications

References 12 publications

Handling of Gliadin Peptides B1‐B4 and of Cow's Milk Proteins by Rat Jejunum Gut Sacs

Handling of Gliadin Peptides B1‐B4 and of Cow's Milk Proteins by Rat Jejunum Gut Sacs

Developmental Regulation of the Inhibitory Effect of Dopamine on Prolactin Release in the Preterm Neonate

Uptake of polypeptide fragments of proteins by rat intestine in vitro and in vivo

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