Stimulating growth and xylindein production of Chlorociboria aeruginascens in agar-based systems

Abstract

Four isolates of Chlorociboria aeruginascens were tested for possible stimulatory effects when grown on malt agar media containing wood additives. The addition of any of the four types of test wood (Acer saccharum, Populus tremuloides, spalted P. tremuloides, and Ailanthus altissima), stimulated colony growth and xylindein production in C. aeruginascens. Addition of any amount of wood produced more growth than no wood additions, while ground wood produced more growth than chopped wood. Of the wood types tested, A. saccharum wood stimulated all four isolates, while spalted Populus tremuloides stimulated three of the four isolates. High glucose and sucrose amounts may be partially responsible for the greater stimulatory affect of some woods over others. The development of this simple and reliable method for growth and pigment stimulation of C. aeruginascens in laboratory conditions will allow for further development of this fungus for decorative and commercial use.

New records of Helotiales in Turkey

“…Also macroscopically, while C. aeruginascens has a number of fruiting bodies in one place, with asymmetric caps and generally stems which are away from the centre and only occasionally attached to the centre, C. aeruginosa has fruiting bodies which form separately, the caps are symmetrical and the stems are generally attached to the centre, and only occasionally away from the centre 17 . C. aeruginascens is reported to have a wide distribution in forested areas in Europe, North America, and Asia 15,20,21,34 . This species grows particularly on many hardwood trees such as Acer sp., Betula sp., Fagus sp., Populus sp., Ulmus sp., and Quercus sp.…”

Wood-Rotting Fungal Pigments as Colorant Coatings on Oil-Based Textile Dyes

“…The pigments were extracted from wood-amended 2% MEA (malt extract agar) malt agar plates, then placed into raw linseed oil as described in Robinson et al [21]. The amended plates contained finely ground white rotted, sterilized wood shavings that stimulate pigment production [22]. Extracted pigments were added to the raw linseed oil in their DCM carrier, and then the DCM was evaporated off, leaving the pigments in oil solution.…”

“…Not only is linseed oil a common carrier used in oil paints [21], but it can also be used as a painting medium, making oil paints more fluid, transparent, and glossy [20]. Preliminary studies [22] of several natural oils-including five types of linseed oil-as potential fungal pigment carriers show that many of these oils were excellent carriers, and specifically that raw linseed exhibited not only a high carrying capacity of the pigments, but also color stability. When mixed with the fungal pigment, raw linseed oil not only does not affect the pigment color, but also allows different pigment colors to be blended to some degree [21,23].…”

Feasibility of Coloring Bamboo with the Application of Natural and Extracted Fungal Pigments

“…This result can be compared with a previous test performed by Robinson [26,34], where S. cuboideum obtained a similar result in sugar maple from six to ten weeks. Interestingly, incubation conditions between this experiment and the previous cited were different-the bamboo was run at 19 ± 2 • C, while the experiment run by Robinson was at 27 ± 2 • C. The selection of a lower temperature was due to the inclusion of C. aeruginosa, which prefers lower growth temperatures (21 ± 2 • C) [13]. Based on this observation, it is possible that with a higher temperature, S. cuboideum would have produced more pigment in a shorter time frame.…”

“…Scytalidium cuboideum, S. ganodermophthorum, and C. aeruginosa were grown on malt agar plates with white-rotted maple wood chips following the protocol established in Robinson et al [13]. Pigments were extracted with dichloromethane (DCM) following the methodology of Robinson et al [18].…”