Stimulated salivary flow rate, pH and lactobacillus and yeast concentrations in non‐smokers and smokers

Parvinen, T

doi:10.1111/j.1600-0722.1984.tb00897.x

Cited by 27 publications

(27 citation statements)

References 14 publications

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“…In fact, 15-year-olds could be considered as orally 'grown up' regarding their oral sugar clearance, which earlier has been shown concerning their salivary secretion rate [Crossner, 1984b]. The mean stimulated salivary flow rate for the adults in this study was approximately twice as high as in other reports [Heintze et al, 1983;Parvinen, 1984] and 1 ml/min higher than for the 15-year-olds in the present study. In spite of this, the oral sugar clearance was similar in these two test groups, sup porting previous reports that variations within the normal-high stimulated salivary flow levels do not in fluence the oral sugar clearance rate significantly [Swenander Lanke, 1957;Hase and Birkhed, 1988;Spiers and Dean, 1989].…”

Section: Discussionsupporting

confidence: 61%

Oral Sugar Clearance in Children Compared with Adults

1991

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The aim of the present investigation was to study oral sugar clearance of various types of foodstuffs in children of different ages in comparison with adults. Thirty children and 20 adults were selected constituting five groups, each containing 10 individuals: 3-, 7-, 15-year-olds and adults, all with normal salivary secretion rate, and adults with low salivary secretion rate (dry mouth patients). Oral sugar clearance was studied using three glucose-containing products: solution, tablet and biscuit. Repeated saliva samples were collected every other minute, between 1 and 11 min after the intake, with a convenient method suitable for very young children. For that purpose, a small circular paper disc, absorbing approximately 20 μl of saliva, was placed under the tongue for 10 s. The glucose concentration of the samples was then analyzed enzymatically. The results showed that, in children, the oral sugar clearance became faster with increasing age. The adults and the 15-year-olds had similar patterns of clearance, while the dry mouth patients had the slowest oral clearance of all groups. The 3- and 7-year-olds showed the greatest variation in oral clearance between the various foodstuffs; the solution had faster clearance than both the biscuit and the tablet. The main conclusion from this study is that young children (3- and 7-year-olds) have both slower salivary sugar clearance and larger variation in clearance among various foodstuffs than older children and adults.

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Section: Discussionsupporting

confidence: 61%

Oral Sugar Clearance in Children Compared with Adults

1991

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“…The biologic plausibility of this finding is supported by the potential negative effects of tobacco use on salivary pH, salivary flow, and oral microbial composition. 32,33 Our finding is consistent with earlier reports. 34,35 However, this association has not always been established in cross-sectional studies.…”

Section: Divaris Et Alsupporting

confidence: 93%

Risk Factors for Third Molar Occlusal Caries: A Longitudinal Clinical Investigation

Divaris

Fisher

Shugars

et al. 2012

Journal of Oral and Maxillofacial Surgery

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“…In one study, an estimate of the fraction of human DNA in DNA extracted from saliva ranged from 11-100% [9]. Two studies have shown increased amounts of specific types of bacteria in saliva of smokers compared to that of nonsmokers [14,15]. A study evaluating the effects of the fraction of human DNA present in DNA extracted from saliva and buccal samples on genotyping using the Affymetrix GeneChip ® Mapping 500K Array suggested that samples containing < 30% human DNA had poor genotyping performance [16].…”

Section: Introductionmentioning

confidence: 99%

Clinical trial participant characteristics and saliva and DNA metrics

Nishita

Jack

McElroy

et al. 2009

BMC Med Res Methodol

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BackgroundClinical trial and epidemiological studies need high quality biospecimens from a representative sample of participants to investigate genetic influences on treatment response and disease. Obtaining blood biospecimens presents logistical and financial challenges. As a result, saliva biospecimen collection is becoming more frequent because of the ease of collection and lower cost. This article describes an assessment of saliva biospecimen samples collected through the mail, trial participant demographic and behavioral characteristics, and their association with saliva and DNA quantity and quality.MethodsSaliva biospecimens were collected using the Oragene® DNA Self-Collection Kits from participants in a National Cancer Institute funded smoking cessation trial. Saliva biospecimens from 565 individuals were visually inspected for clarity prior to and after DNA extraction. DNA samples were then quantified by UV absorbance, PicoGreen®, and qPCR. Genotyping was performed on 11 SNPs using TaqMan® SNP assays and two VNTR assays. Univariate, correlation, and analysis of variance analyses were conducted to observe the relationship between saliva sample and participant characteristics.ResultsThe biospecimen kit return rate was 58.5% among those invited to participate (n = 967) and 47.1% among all possible COMPASS participants (n = 1202). Significant gender differences were observed with males providing larger saliva volume (4.7 vs. 4.5 ml, p = 0.019), samples that were more likely to be judged as cloudy (39.5% vs. 24.9%, p < 0.001), and samples with greater DNA yield as measured by UV (190.0 vs. 138.5, p = 0.002), but reduced % human DNA content (73.2 vs. 77.6 p = 0.005) than females. Other participant characteristics (age, self-identified ethnicity, baseline cigarettes per day) were associated with saliva clarity. Saliva volume and saliva and DNA clarity were positively correlated with total DNA yield by all three quantification measurements (all r > 0.21, P < 0.001), but negatively correlated with % human DNA content (saliva volume r = -0.148 and all P < 0.010). Genotyping completion rate was not influenced by saliva or DNA clarity.ConclusionFindings from this study show that demographic and behavioral characteristics of smoking cessation trial participants have significant associations with saliva and DNA metrics, but not with the performance of TaqMan® SNP or VNTR genotyping assays.Trial registrationCOMPASS; registered as NCT00301145 at clinicaltrials.gov.

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Stimulated salivary flow rate, pH and lactobacillus and yeast concentrations in non‐smokers and smokers

Cited by 27 publications

References 14 publications

Oral Sugar Clearance in Children Compared with Adults

Oral Sugar Clearance in Children Compared with Adults

Risk Factors for Third Molar Occlusal Caries: A Longitudinal Clinical Investigation

Clinical trial participant characteristics and saliva and DNA metrics

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