Stimulated Raman photoacoustic imaging

Yakovlev, Vladislav V.; Zhang, Hao F.; Noojin, Gary D.; Denton, Michael L.; Thomas, Robert J.; Scully, Marlan O.

doi:10.1073/pnas.1012432107

Cited by 63 publications

(43 citation statements)

References 41 publications

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“…This, combined with the intense nature of stimulated Raman scattering (SRS) leads to the possibility of remote detection of powders, such as fertilizers or anthrax [3]. Deep-tissue SRS biomedical imaging can benefit as well [4]. Monte Carlo simulations [5,6] were used to guide our experimental efforts, and we experimentally achieved microjoule-level, gigawatt peak power, emission from such a random Raman laser.…”

Section: Introductionmentioning

confidence: 99%

Random Raman laser shines bright

Hokr

Noojin²,

Beier

et al. 2013

CLEO: 2013 Postdeadline

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Section: Introductionmentioning

confidence: 99%

Random Raman laser shines bright

Hokr

Noojin²,

Beier

et al. 2013

CLEO: 2013 Postdeadline

Self Cite

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“…The growing availability of tunable laser diodes with exceptional short-and long-term stability will allow a faster penetration of SRS microscopy into biomedical and materials science research. The same concept can be also extended for stimulated Raman photoacoustic imaging [42,43].…”

Section: Discussionmentioning

confidence: 96%

Stimulated Raman microscopy without ultrafast lasers

2013

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Stimulated Raman scattering (SRS) microscopy is a powerful tool for chemically-sensitive non-invasive optical imaging. However, the short-pulse laser sources, which are currently being employed for this imaging technique, are still expensive and require substantial maintenance to provide temporal and spectral overlap. SRS imaging, which utilizes cw laser sources, has a major advantage over pulsed lasers, as it eliminates the possibility of cell damage due to exposure to high-intensity light radiation, while substantially reducing the cost and complexity of the set-up and keeping a subcellular spatial resolution. As a proof-of-principle, we demonstrate microscopic imaging of dimethyl sulfoxide using two independent, commonly used and inexpensive lasers: a diode-pumped, intracavity doubled 532 nm laser and a He-Ne laser operating at 633 nm. In our proof-of-principle experience, dimethyl sulfoxide acts as a contrast agent providing Raman scattering signal. The 532 nm and 633 nm lasers act as excitation and probe sources, respectively [1].

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“…In 2003, we introduced pulsed PT nanotherapy (also termed selective nanophotothermolysis) for killing individual cancer cells and bacteria targeted by strongly absorbing NPs (19,22–24,115,116). The killing mechanism was related to the formation of nano- and microbubbles around laser-overheated NPs, which during expansion mechanically damaged cellular structures.…”

Section: Application Of Cnt and Its Hybridmentioning

confidence: 99%

Advanced contrast nanoagents for photoacoustic molecular imaging, cytometry, blood test and photothermal theranostics

Zerda

Kim

Galanzha

et al. 2011

Contrast Media & Molecular

106

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Various nanoparticles have raised significant interest over the past decades for their unique physical and optical properties and biological utilities. Here we summarize the vast applications of advanced nanoparticles with a focus on carbon nanotube (CNT)-based or CNT-catalyzed contrast agents for photoacoustic (PA) imaging, cytometry and theranostics applications based on the photothermal (PT) effect. We briefly review the safety and potential toxicity of the PA/PT contrast nanoagents, while showing how the physical properties as well as multiple biological coatings change their toxicity profiles and contrasts. We provide general guidelines needed for the validation of a new molecular imaging agent in living subjects, and exemplify these guidelines with single-walled CNTs targeted to αvβ3, an integrin associated with tumor angiogenesis, and golden carbon nanotubes targeted to LYVE-1, endothelial lymphatic receptors. An extensive review of the potential applications of advanced contrast agents is provided, including imaging of static targets such as tumor angiogenesis receptors, in vivo cytometry of dynamic targets such as circulating tumor cells and nanoparticles in blood, lymph, bones and plants, methods to enhance the PA and PT effects with transient and stationary bubble conjugates, PT/PA Raman imaging and multispectral histology. Finally, theranostic applications are reviewed, including the nanophotothermolysis of individual tumor cells and bacteria with clustered nanoparticles, nanothrombolysis of blood clots, detection and purging metastasis in sentinel lymph nodes, spectral hole burning and multiplex therapy with ultrasharp rainbow nanoparticles.

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Stimulated Raman photoacoustic imaging

Cited by 63 publications

References 41 publications

Random Raman laser shines bright

Random Raman laser shines bright

Stimulated Raman microscopy without ultrafast lasers

Advanced contrast nanoagents for photoacoustic molecular imaging, cytometry, blood test and photothermal theranostics

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