Stigmasterol: An adjuvant for beta lactam antibiotics against beta-lactamase positive clinical isolates

Tong, Woei Yenn; Khan, Minhaj Ahmad; Syuhada, Nur Amiera; Ring, Leong Chean; Demirtaş, İbrahim; Tan, Wen-Nee

doi:10.1016/j.steroids.2017.10.016

Cited by 26 publications

(22 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They found that stigmasterol could prevent the tumor growth by disrupting tumor angiogenesis, which mainly functioned by selectively suppressing the viability, migration, and morphogenesis of human umbilical vein endothelial cells via downregulation of tumor necrosis factor-α and VEGFR-2 17. Yenn et al proved that stigmasterol could serve as an antibiotic adjuvant to improve the effect of ampicillin against infections caused by β-lactamase producing pathogens 18. Antwi et al found that 50 mg/kg and 100 mg/kg of stigmasterol would be helpful to inhibit lipopolysaccharide-induced innate immune responses and improve survival up to 40% in murine models 19…”

Section: Introductionmentioning

confidence: 99%

<p>Stigmasterol Exerts Neuro-Protective Effect Against Ischemic/Reperfusion Injury Through Reduction Of Oxidative Stress And Inactivation Of Autophagy</p>

Sun¹,

Li²,

Liu³

et al. 2019

NDT

View full text Add to dashboard Cite

PurposeStroke remains the primary cause of pain, suffering, and death in patients. One of the major thrusts in stroke therapy is to find an effective prevention strategy. Objectives of this study are to testify the neuro-protection effect of stigmasterol in ischemic/reperfusion injury model.MethodsThe dosage-dependent effects (20, 40, and 80 mg/kg) of stigmasterol on physiological behaviors and oxidative stress biomarkers were investigated. Expression and phosphorylation of beclin1, microtubule-associated protein 1 light chain 3 (LC3), adenosine monophosphate-activated protein kinase (AMPK), mTOR, and N-terminal kinase (JNK) were detected.ResultsThe results showed that stigmasterol was able to effectively reduce neurological deficits and infarct damage induced by the ischemic/reperfusion injury, improve histopathology changes, and restore the levels of the endogenous antioxidant defense system in a dose–response mode. Stigmasterol effectively depressed the expression level of beclin1, and the conversion of LC3 I to LC3 II, while promoted the phosphorylation of mTOR, and remarkably inhibited the phosphorylation of AMPK and JNK, as well as the expression of JNK induced by 24 hrs of reperfusion.ConclusionThese findings reveal that stigmasterol has neuro-protective effect against the ischemic/reperfusion injury, possibly associated with reduction of oxidative stress and inactivation of autophagy via AMPK/mTOR and JNK pathways.

show abstract

Section: Introductionmentioning

confidence: 99%

<p>Stigmasterol Exerts Neuro-Protective Effect Against Ischemic/Reperfusion Injury Through Reduction Of Oxidative Stress And Inactivation Of Autophagy</p>

Sun¹,

Li²,

Liu³

et al. 2019

NDT

View full text Add to dashboard Cite

show abstract

“…Stigmasterol exerted the synergistic antibiotic effect (suppressed colony counts, yielding 98.7%) as an adjuvant of ampicillin to gram-negative and gram-positive bacteria. However, the inhibition of ampicillin and stigmasterol alone was ineffective ( Yenn et al, 2017 ). The aqueous extract of A. lancea has the activity of anti-monilial infection.…”

Section: Pharmacologymentioning

confidence: 99%

“… Yu et al (2017) Anti-inflammatory effects Water extracts of A. lancea rhizomes 100, 200 mg/kg Male C57BL/6 mice Inducing the hepatic expression of CYP3A Yuan et al (2017) Alleviating LPS-induced inflammatory responses Atractylodin 40 or 80 mg/kg Lipopolysaccharide (LPS)-induced inflammatory rates Suppression of nucleotide-binding domain-like receptor protein 3 inflammasome and toll-like receptor 4 activation Tang et al (2018) Ameliorate colitis β-sitosterol and stigmasterol 0.4% Dextran sulfate sodium (DSS)-induced colitis in C57BL/6J male mice Suppressing the activation of inflammatory master regulator NF-κB. Stigmasterol significantly lowered colonic inflammation score and the expression of cyclooxygenase-2 and colony stimulating factor-1 Feng et al (2017) Anti-bacterial activity Antimycotic effect AR 0.5-10.0% Trichophyton tonsuraus , Microsporum gypseum , et al This drug had apparent fungi action Yin et al (2000) Combating multidrug resistant strains Stigmasterol 1 μg/disc, or in combination (1 μg/disc ampicillin + 1 μg/disc stigmasterol) 2 gram-positive bacteria and 2 gram-negative bacteria Synergistic effects of stigmasterol and ampicillin against β-lactamase producing clinical isolates Yenn et al (2017) Other activities Protective effects of acute lung injury LPS+AO-I (5, 10 and 20 mg/kg), LPS+VGX-1027 (0.5 mg/mouse) LPS-induced acute lung injury mouse model Inhibiting of TLR4 expression and NF-κB activation Zhang et al (2015) Antiasthmatic properties Stigmasterol 10-100 mg/kg Ovalbumin-induced Guinea pigs Reducing the proliferation of eosinophils, lymphocytes, and monocytes while reducing peribronchiolar, perivascular, and alveolar ...…”

Section: Pharmacologymentioning

confidence: 99%

Atractylodis Rhizoma: A review of its traditional uses, phytochemistry, pharmacology, toxicology and quality control

Zhang

Zhao

Chang

et al. 2021

Journal of Ethnopharmacology

View full text Add to dashboard Cite

Ethnopharmacological relevance Atractylodis Rhizoma (AR), mainly includes Atractylodes lancea (Thunb.) DC. ( A. lancea ) and Atractylodes chinensis (DC.) Koidz. ( A. chinensis ) is widely used in East Asia as a diuretic and stomachic drug, for the treatment of rheumatic diseases, digestive disorders, night blindness, and influenza as it contains a variety of sesquiterpenoids and other components of medicinal importance. Aim of the review A systematic summary on the botany, traditional uses, phytochemistry, pharmacology, toxicology, and quality control of AR was presented to explore the future therapeutic potential and scientific potential of this plant. Materials and methods A review of the literature was performed by consulting scientific databases including Google Scholar, Web of Science, Baidu Scholar, Springer, PubMed, ScienceDirect, CNKI, etc. Plant taxonomy was confirmed to the database “The Plant List”. Results Over 200 chemical compounds have been isolated from AR, notably sesquiterpenoids and alkynes. Various pharmacological activities have been demonstrated, especially improving gastrointestinal function and thus allowed to assert most of the traditional uses of AR. Conclusions The researches on AR are extensive, but gaps still remain. The molecular mechanism, structure-activity relationship, potential synergistic and antagonistic effects of these components need to be further elucidated. It is suggested that further studies should be carried out in the aspects of comprehensive evaluation of the quality of medicinal materials, understanding of the “effective forms” and “additive effects” of the pharmacodynamic substances based on the same pharmacophore of TCM, and its long-term toxicity in vivo and clinical efficacy.

show abstract

“…Interestingly, reserpine and quercetin inhibited efflux pump and the application of quinine and morin reversed the antibiotic tolerance induced by sub-lethal concentrations of ciprofloxacin. Phytosterol stigmasterol in combination with ampicillin showed up to 98.7% inhibition of clinical gram positive and gram negative bacteria (Yenn et al, 2017). In addition to phytochemicals, biosurfactants of various origins have also exhibited effective antibiotic adjuvant properties.…”

Section: Combinatorial Treatments To Combat Multidrug Resistancementioning

confidence: 99%

Current Trends in Experimental and Computational Approaches to Combat Antimicrobial Resistance

et al. 2020

View full text Add to dashboard Cite

A multitude of factors, such as drug misuse, lack of strong regulatory measures, improper sewage disposal, and low-quality medicine and medications, have been attributed to the emergence of drug resistant microbes. The emergence and outbreaks of multidrug resistance to last-line antibiotics has become quite common. This is further fueled by the slow rate of drug development and the lack of effective resistome surveillance systems. In this review, we provide insights into the recent advances made in computational approaches for the surveillance of antibiotic resistomes, as well as experimental formulation of combinatorial drugs. We explore the multiple roles of antibiotics in nature and the current status of combinatorial and adjuvant-based antibiotic treatments with nanoparticles, phytochemical, and other non-antibiotics based on synergetic effects. Furthermore, advancements in machine learning algorithms could also be applied to combat the spread of antibiotic resistance. Development of resistance to new antibiotics is quite rapid. Hence, we review the recent literature on discoveries of novel antibiotic resistant genes though shotgun and expression-based metagenomics. To decelerate the spread of antibiotic resistant genes, surveillance of the resistome is of utmost importance. Therefore, we discuss integrative applications of whole-genome sequencing and metagenomics together with machine learning models as a means for state-of-the-art surveillance of the antibiotic resistome. We further explore the interactions and negative effects between antibiotics and microbiomes upon drug administration.

show abstract

Stigmasterol: An adjuvant for beta lactam antibiotics against beta-lactamase positive clinical isolates

Cited by 26 publications

References 18 publications

<p>Stigmasterol Exerts Neuro-Protective Effect Against Ischemic/Reperfusion Injury Through Reduction Of Oxidative Stress And Inactivation Of Autophagy</p>

<p>Stigmasterol Exerts Neuro-Protective Effect Against Ischemic/Reperfusion Injury Through Reduction Of Oxidative Stress And Inactivation Of Autophagy</p>

Atractylodis Rhizoma: A review of its traditional uses, phytochemistry, pharmacology, toxicology and quality control

Current Trends in Experimental and Computational Approaches to Combat Antimicrobial Resistance

Contact Info

Product

Resources

About