Sterols in Ascaris lumbricoides

Cole, Robert; Krusberg, L. R.

doi:10.1016/0010-406x(67)90120-x

Cited by 16 publications

(2 citation statements)

References 10 publications

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“…Others (HIEB & ROTHSTEIN, 1968;COLE & DUTKY, 1969;BARRETT et al, 1970;GERASIMOVA & LEUTSKAYA, 1983;CHITWOOD et al, 1986) maintain that nematodes are capable of steroid synthesis if provided with adequate precursor molecules. It is known that steroids are present within the tissues of nematodes (COLE & KRUSBERG, 1967;BARRETT et al, 1970;DENNIS, 1977;BOTTJER et al, 1984b). If steroids are essential to the reproductive biology of nematodes it is unlikely that they would rely entirely on host-provided steroids, as it would therefore be expected that there would be even more pronounced differences in egg output by parasites from male or female hosts or from adult or juvenile hosts.…”

Section: Discussionmentioning

confidence: 99%

“…The presence of hormones in nematodes has been well documented (COLE & KRUSBERG, 1967;BARRETT et al, 1970;DENNIS, 1977;BOTTJER et al, 1984b;CHUNG et al, 1986). Also, the effect of hormones such as testosterone, estradiol, ecdysone, and others on overall growth and development, both in vivo (SOLOMON, 1966;REDDINGTON et al, 1981;CHOMICZ, 1984;SWANSON et al, 1984;KHATOON et al, 1986), and in vitro (Lu et al, 1977;BOTTJER et al, 1984a) has been investigated.…”

Section: Introductionmentioning

confidence: 99%

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Heligmosomoides polygyrus: effect of exogenous steroid hormones on egg output in vitro

Richardson

MacKinnon

1990

J. Helminthol.

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The effect of exogenous steroid hormones on the egg output of Heligmosomoides polygyrus (Nematoda: Trichostrongylidae) was examined in vitro. Using worms raised in female mice, it was found that estradiol, testosterone, and cortisone each significantly decreased egg output. Although similar trends were found using H. polygyrus raised in male mice, none of the decreases found was significant. No significant differences were found with ecdysone or progesterone treatments using worms from female or male mice. Treatment of worms with cortisone did not significantly affect retention of eggs within the uterus of H. polygyrus. Titration of the effect of cortisone on egg output indicated that levels of reduction were significant for concentrations of 5·6 × 10−6 M to 5·6 × 10−3 M in worms from female mice and for concentrations of 5·6 × 10−8, × 10−7, × 10−5 and × 10−3 in worms from male mice. Radioisotope labelling experiments showed incorporation of 3H-corticosterone in the nucleus of intestinal cells of H. polygyrus suggesting that its effect on egg production may be via a modulatory effect on the intestinal cells.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Heligmosomoides polygyrus: effect of exogenous steroid hormones on egg output in vitro

Richardson

MacKinnon

1990

J. Helminthol.

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Glycerides, waxes and sterols in ovaries ofAscaris lumbricoides (Nematoda)

Tarr¹,

Fairbairn²

1973

Lipids

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Glycerides (65%), ascaroside esters (33%), waxes (1.5%) and sterols (0.26%) accounted for essentially all the neutral lipids inA. lumbricoides ovaries. Nineteen per cent by weight of the triglycerides contained only long chain fatty acids. Nearly all the remaining triglycerides contained 1 mol volatile acid. Mono‐ and diglycerides, free fatty acids and triglycerides containing 2 mol volatile acids were present in very small amounts. Mole percentages of glyceride volatile acids were α‐methylavaleric (70), α‐methylbutyric (23),n‐valeric (ca. 7), and traces of acetic, propionic, isobutyric andn‐butyric. Mole percentages of the alcoholic component of the waxes were 96% 1‐octadecanol, and 4% of its 16, 17 and 19 carbon homologs. The acid components were α‐methylbutyric (75 mol%), acetic (16 mol%), propionic andn‐valeric (each 4 mol%), and traces ofn‐butyric and α‐methylvaleric. Sterols (42 wt% cholesterol, 30% cholestanol, 9.5% campestanol, 8.2% stigmastanol, 6.6% β‐sitosterol, and 3.8% campesterol) were essentially the same as those found in the whole worm, except that no esterified sterols were present.

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Sterol metabolism in the nematodeCaenorhabditis elegans

Chitwood

Lusby

Lozano

et al. 1984

Lipids

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The metabolism of various dietary sterols and the effects of an azasteroid on sitosterol metabolism in the free-living nematodeCaenorhabditis elegans was investigated. The major unesterified sterols ofC. elegans in media supplemented with sitosterol, cholesterol or desmosterol included 7-dehydrocholesterol (66.5%, 40.5%, 31.2%, respectively), cholesterol (6.7%, 52.3%, 26.9%), lathosterol (4.4%, 3.6%, 1.7%) and 4α-methylcholest-8(14)-en-3β-ol (4.2%, 2.1%, 3.8%). Esterified sterols, representing less than 20% of the total sterols, were somewhat similar except for a significantly higher relative content of 4α-methylcholest-8(14)-en-3β-ol (23.3%, 23.4%, 10.6%). ThusC. elegans not only removes the substituent at C24 of dietary sitosterol but possesses the unusual ability to produce significant quantities of 4α-methylsterols. WhenC. elegans was propagated in medium supplemented with sitosterol plus 5 μg/ml of 25-azacoprostane hydrochloride, the azasteroid strongly interfered with reproduction and motility ofC. elegans and strongly inhibited the Δ24-sterol reductase enzyme system; excluding sitosterol, the major free sterols of azacoprostane-treatedC. elegans were cholesta-5, 7, 24-trien-3β-ol (47.9%), desmosterol (9.4%), fucosterol (2.1%) and cholesta-7,24-dien-3β-ol (2.0%). These 4 sterols are likely intermediates in the metabolism of sitosterol inC. elegans.

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Sterols in Ascaris lumbricoides

Cited by 16 publications

References 10 publications

Heligmosomoides polygyrus: effect of exogenous steroid hormones on egg output in vitro

Heligmosomoides polygyrus: effect of exogenous steroid hormones on egg output in vitro

Glycerides, waxes and sterols in ovaries ofAscaris lumbricoides (Nematoda)

Sterol metabolism in the nematodeCaenorhabditis elegans

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