Glycerides, waxes and sterols in ovaries ofAscaris lumbricoides (Nematoda)

Abstract

Glycerides (65%), ascaroside esters (33%), waxes (1.5%) and sterols (0.26%) accounted for essentially all the neutral lipids inA. lumbricoides ovaries. Nineteen per cent by weight of the triglycerides contained only long chain fatty acids. Nearly all the remaining triglycerides contained 1 mol volatile acid. Mono‐ and diglycerides, free fatty acids and triglycerides containing 2 mol volatile acids were present in very small amounts. Mole percentages of glyceride volatile acids were α‐methylavaleric (70), α‐methylbutyric (23),n‐valeric (ca. 7), and traces of acetic, propionic, isobutyric andn‐butyric. Mole percentages of the alcoholic component of the waxes were 96% 1‐octadecanol, and 4% of its 16, 17 and 19 carbon homologs. The acid components were α‐methylbutyric (75 mol%), acetic (16 mol%), propionic andn‐valeric (each 4 mol%), and traces ofn‐butyric and α‐methylvaleric. Sterols (42 wt% cholesterol, 30% cholestanol, 9.5% campestanol, 8.2% stigmastanol, 6.6% β‐sitosterol, and 3.8% campesterol) were essentially the same as those found in the whole worm, except that no esterified sterols were present.

Long‐Chain O‐Ascarosyl‐alkanediols Are Constitutive Components of Caenorhabditis elegans but Do Not Induce Dauer Larva Formation

“…1%. This is in contrast with what was observed in parasitic nematodes, where the ascaroside esters constituted up to 33% of all neutral lipids in the ovaries [9].…”

“…The dauer larva induction assay was performed according to [9], with monoascarosyl alkanediol (25 or 250 mg per well) isolated from A. suum.…”

Biosynthesis of lipids during embryogenesis of Ascaris lumbricoides eggs.

“…The concentrations of the branched C15 fatty acid, are 1.6 to 2.0% of the total fatty acid in eggs at all stages of embryonation. This observation is consistent with the results previously mentioned by Jezyk (1968) and Tarr and Fairbairn (1973), who reported 1.9% and 1.24% of the fatty acid in ovarian triglyceride of Ascaris him bricoides, respectively. In aerobic conditions, it was observed that the incorporation of radioactivity in C18,1 acid was decreased and that in branched C15 fatty acid was greatly increased in the second stage larvae compared with the first stage larvae.…”