Steroidal saponins and sapogenins from fenugreek and their inhibitory activity against α-glucosidase

Zhang, Huixing; Xu, Jing; Wang, Meizhe; Xia, Xiaoyan; Dai, Rongke; Zhao, Yanfang

doi:10.1016/j.steroids.2020.108690

Cited by 32 publications

(16 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…12,13 Similarly, fenugreek has been reported to regulate the levels of triglycerides, total cholesterol, and low-density lipoprotein (LDL) as well. [14][15][16][17] It was observed that saponins, one of the main constituents of fenugreek, transforms into sapogenins in the gastrointestinal tract and decreases the level of serum cholesterol. 14,18 Fenugreek demonstrated the antidiabetic potential by stimulating the secretion of insulin and decreasing insulin resistance in tissues.…”

Section: Introductionmentioning

confidence: 99%

“…[14][15][16][17] It was observed that saponins, one of the main constituents of fenugreek, transforms into sapogenins in the gastrointestinal tract and decreases the level of serum cholesterol. 14,18 Fenugreek demonstrated the antidiabetic potential by stimulating the secretion of insulin and decreasing insulin resistance in tissues. It also described that fenugreek seeds activated insulin receptor substrate-associated phosphoinositide 3 kinase (PI3K) activity.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Methanolic Fenugreek Seed Extract Induces p53-Dependent Mitotic Catastrophe in Breast Cancer Cells, Leading to Apoptosis

Khan¹,

Allemailem

Alsahli

et al. 2021

JIR

View full text Add to dashboard Cite

Purpose The plant Trigonella foenum-graecum , well-known as fenugreek, has been shown to control type-2 diabetes, the level of cholesterol, inflammation of wounds, disorders related to gastrointestinal tracts, and cancer as well. The present study aimed to evaluate the anti-cancer potential of methanolic fenugreek seed extract (FSE) and its possible molecular mechanism of action in breast cancer cells. Methods The anticancer potential of FSE was evaluated in MCF-7 and SK-BR3 breast cancer cells through various cellular assays after selecting the IC 10 , IC 25 , IC 35 , and IC 50 doses by the cell cytotoxicity assay. Furthermore, the oral acute toxicity of FSE was examined in mice, according to the guidelines of the Organization for Economic Co-operation and Development (OECD). Results FSE exhibited dose-dependent cytotoxicity, as the IC 50 was found to be 150 and 40 μg/mL for MCF-7 and SK-BR3 breast cancer cells, respectively. The cytological observations showed the typical apoptotic morphology in both of the breast cancer cells upon treatment with FSE, as it inhibited the migration and adhesion, in a dose-dependent manner. The flow cytometry analysis revealed that FSE induced a significant shift from G 2 /M, and polyploidy (>G) at higher concentrations that suggested the activation of p53-mediated mitotic catastrophe, consequently leading to apoptosis. FSE induced a significant increase in the mitochondrial depolarization, ROS as well as a Bax/Bcl-2 ratio, and also exhibited the mitochondrial associated p53 signaling pathway. The in vivo acute toxicity data revealed that the oral administration of FSE did not induce any toxic effect in mice. Conclusion This study, for the first time, reports the mechanistic details of the anti-cancer potential of FSE. It requires a detailed analysis to understand the effect of FSE to induce the apoptosis through the multiple signaling pathways at varying concentrations. The nontoxic effect of FSE in mice suggests to utilize it safely for pharmaceutical formulations in different cancer systems.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Methanolic Fenugreek Seed Extract Induces p53-Dependent Mitotic Catastrophe in Breast Cancer Cells, Leading to Apoptosis

Khan¹,

Allemailem

Alsahli

et al. 2021

JIR

View full text Add to dashboard Cite

show abstract

“…S. Sharma and V. Mishra et al were found the protective effect on the DNA damage by removing free radicals that cause oxidative stress [47]. Trigonella foenum seeds were high in saponins, notably dioscin or diosgenin, which had been isolated and screened for α-glucosidase inhibitory activity in vitro, confirming natural anti-diabetic agents with minor toxicity [48]. (9Z,12Z)-N-((3R,4R,5S)-4,5-Dimethyl-2-oxotetrahydrofur-3-yl) octadeca-9,12dienamide (N55) , a positive GLP-1 signaling modulator obtained from fenugreek seeds was reported as effective for treatment of diabetes [49].…”

Section: Trigonella Foenummentioning

confidence: 98%

Role of Medicinal Plants in the Management of Diabetes Mellitus: A Review

Sharma

Bora

2021

JPRI

View full text Add to dashboard Cite

Diabetes mellitus is a worse metabolic condition in which level of glucose and lipid increased in blood circulation due to insufficient insulin functionality that may lead to worse the complication like atherosclerosis, dyslipidemia, gangrene, limb amputation, kidney failure, and many more. Furthermore, high concentration of glucose in blood stream can directly produce reactive oxygen species which ultimately more worsen the conditions like dyslipidemia, atherosclerosis etc. This disorder is expected to affect 9.3% of the global population in 2019, increasing to 10.2% by 2030 and increasing 10.9 % by 2045. Medicinal plants are used as medicine to treat many ailments since a long time ago. But now they have been rediscovered with having specific constituents to treat diseases and many more yet to discover. There are so many active phytoconstituents of plants which are being extracted, purified, tested and formulated for patient convenience. In the present review, various plants used to treat diabetes mellitus due to have negligible side effects as compared to allopathic treatment (oral hypoglycemic drugs) are described. Many plants with hypoglycemic effect of many plants have been reported, and the mechanisms of action of these plants with these hypoglycemic behaviors are being examined through in vitro and in vivo studies. Some of these 22 medicinal plants and their active constituents related to antidiabetic activity are discussed in this review.

show abstract

“…Supporting this finding, saponins from Momordica charantia showed potential antidiabetic effects in a T2D rat model induced by a high fat diet-STZ through suppressing stress oxidative pathways (inducing the activities of SOD and CAT enzymes while decreasing MDA levels in the liver and pancreas), modulating insulin signaling pathways (promoting the tyrosine phosphorylation of IRS-1 and Akt Ser-473 while reducing the serine phosphorylation of IRS-1), and attenuating metabolic markers (reducing non-esterified fatty acid, triglyceride, and total cholesterol) [145]. In vitro studies have shown steroidal saponins and sapogenins from fenugreek potential as hypoglycemic agents through their role in promoting the α-glucosidase enzyme [146]. Saponins from Stauntonia chinensis was reported to exert hypoglycemic activity by lowering blood glucose, improving insulin response, and increasing glycogen synthesis through induction of GLUT4 involved in the activation of the IRS1/PI3K/ Akt signaling pathway in dB/dB mice.…”

Section: Phytochemical Compounds Of S Macrophylla Seeds Responsible For Hypoglycemic Effectsmentioning

confidence: 99%

The Molecular Mechanisms of Hypoglycemic Properties and Safety Profiles of Swietenia Macrophylla Seeds Extract: A Review

Yudhani

Nugrahaningsih

Sholikhah

et al. 2021

Open Access Maced J Med Sci

View full text Add to dashboard Cite

BACKGROUND: Insulin resistance (IR) is known as the root cause of type 2 diabetes; hence, it is a substantial therapeutic target. Nowadays, studies have shifted the focus to natural ingredients that have been utilized as a traditional diabetes treatment, including Swietenia macrophylla. Accumulating evidence supports the hypoglycemic activities of S. macrophylla seeds extract, although its molecular mechanisms have yet to be well-established. AIM: This review focuses on the hypoglycemic molecular mechanisms of S. macrophylla seeds extract and its safety profiles. METHODS: An extensive search of the latest literature was conducted from four main databases (PubMed, Scopus, Science Direct, and Google Scholar) using several keywords: “swietenia macrophylla, seeds, and diabetes;” “swietenia macrophylla, seeds, and oxidative stress;” “swietenia macrophylla, seeds, and inflammation;” “swietenia macrophylla, seeds, and GLUT4;” and “swietenia macrophylla, seeds, and toxicities.” RESULTS: The hypoglycemic activities occur through modulating several pathways associated with IR and T2D pathogenesis. The seeds extract of S. macrophylla modulates oxidative stress by decreasing malondialdehyde (MDA), oxidized low-density lipoprotein, and thiobarbituric acid-reactive substances while increasing antioxidant enzymes (superoxide dismutase, glutathione peroxidase, and catalase). Another propose mechanism is the modulating of the inflammatory pathway by attenuating nuclear factor kappa β, tumor necrosis factor α, inducible nitric oxide synthase, and cyclooxygenase 2. Some studies have shown that the extract can also control phosphatidylinositol-3-kinase/ Akt (PI3K/Akt) pathway by inducing glucose transporter 4, while suppressing phosphoenolpyruvate carboxykinase. Moreover, in vitro cytotoxicity and in vivo toxicity studies supported the safety profile of S. macrophylla seeds extract with the LD50 higher than 2000 mg/kg. CONCLUSION: The potential of S. macrophylla seeds as antidiabetic candidate is supported by many studies that have documented their non-toxic and hypoglycemic effects, which involve several molecular pathways.

show abstract

Steroidal saponins and sapogenins from fenugreek and their inhibitory activity against α-glucosidase

Cited by 32 publications

References 24 publications

Methanolic Fenugreek Seed Extract Induces p53-Dependent Mitotic Catastrophe in Breast Cancer Cells, Leading to Apoptosis

Methanolic Fenugreek Seed Extract Induces p53-Dependent Mitotic Catastrophe in Breast Cancer Cells, Leading to Apoptosis

Role of Medicinal Plants in the Management of Diabetes Mellitus: A Review

The Molecular Mechanisms of Hypoglycemic Properties and Safety Profiles of Swietenia Macrophylla Seeds Extract: A Review

Contact Info

Product

Resources

About