Steroidal saponins and lignan glycosides from the rhizomes of Paris polyphylla var. latifolia

Dong, Hua; Zhang, Wei; Lu, Yang; Liu, Yang; Wang, Xia-Yin; Li, Hui; Tang, Hai‐Feng

doi:10.1016/j.bse.2018.09.005

Cited by 4 publications

(3 citation statements)

References 11 publications

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“…latifolia [ 10 ], a variant of the genus Paris , is widely distributed in southwest China. To date, it has only been previously studied in our research group [ 11 , 12 ]. Therefore, this study focused on the cytotoxic steroidal saponins in Paris polyphylla var.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic Steroidal Saponins Containing a Rare Fructosyl from the Rhizomes of Paris polyphylla var. latifolia

Wang³

et al. 2023

IJMS

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A phytochemical investigation of the steroidal saponins from the rhizomes of Paris polyohylla var. latifolia led to the discovery and characterization of three new spirostanol saponins, papolatiosides A–C (1–3), and nine known compounds (4–12). Their structures were established via extensive spectroscopic data analysis and chemical methods. Interestingly, compounds 1 and 2 possessed a fructosyl in their oligosaccharide moiety, which is rare in natural product and was firstly reported in family Melanthiaceae. The cytotoxicity of these saponins against several human cancer cell lines was evaluated by a CCK-8 experiment. As a result, compound 1 exhibited a significant cytotoxic effect on LN229, U251, Capan-2, HeLa, and HepG2 cancer cells with IC50 values of 4.18 ± 0.31, 3.85 ± 0.44, 3.26 ± 0.34, 3.30 ± 0.38 and 4.32 ± 0.51 μM, respectively. In addition, the result of flow cytometry analysis indicated that compound 1 could induce apoptosis of glioma cells LN229. The underlying mechanism was explored by network pharmacology and western bolt experiments, which indicated that compound 1 could induce glioma cells LN229 apoptosis by regulating the EGFR/PI3K/Akt/mTOR pathway.

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Section: Introductionmentioning

confidence: 99%

Cytotoxic Steroidal Saponins Containing a Rare Fructosyl from the Rhizomes of Paris polyphylla var. latifolia

Wang³

et al. 2023

IJMS

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“…14,15 As the accurate and rapid analysis function, ultra-high performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-ESI-Q-TOF-MS) has been employed widely in qualitative and quantitative research on chemical components. 16,17 UPLC-ESI-Q-TOF-MS demonstrates excellent separation efficiency and strong structural characterization capability, and can not only give the molecular weight, structural information but also the qualitative analysis of chemical components. [18][19][20] In this work, we hope to establish HPLC fingerprint chromatography of the MPPY to analyze and assign the common peaks and then combine UPLC-ESI-Q-TOF-MS technology to evaluate comprehensively the quality of MPPY.…”

Section: Introductionmentioning

confidence: 99%

A Simple and Efficient UPLC-ESI-Q-TOF-MS Method for Determining the Chemical Composition in Rhizomes of Multiple Stems Paris polyphylla var. yunnanensis

He,

Jin,

Wang

et al. 2023

Natural Product Communications

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Due to the clinical significance of steroidal saponins, multiple stems Paris polyphylla var. yunnanensis (MPPY) has attracted much interest and played a crucial role in drug development. However, developing a simple and efficient method to analyze the chemical composition of MPPY remains a pronounced challenge. Herein, we report for the first time the component analysis of MPPY rhizomes using the chromatographic fingerprint combined with ultra-high performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-ESI-Q-TOF-MS) method. Nineteen compositions of MPPY were qualitatively investigated by such strategy and confirmed as steroidal saponins. Among these compositions, 17 steroidal saponins were detected in 10 different batches of MPPY. Thirteen common peaks appeared in chromatographic fingerprint, of which 11 common peaks can be attributed by LC–MS data. The experimental facts demonstrate that the method established is simple, rapid, and reliable to evaluate the quality of MPPY. Besides, the correlation and difference of steroidal saponins in the rhizomes of MPPY from different places were also studied.

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“…However, with the rapid development of the planting industry and changes in ecological and environmental factors such as blind introduction and the planting mode, the quality of P. polyphylla has deteriorated, and the disease of P. polyphylla has become increasingly prominent, especially root rot [7]. During the long growth process of P. polyphylla, any diseases can lead to crop failure, which is the highest risk source for P. polyphylla cultivation and seriously restricts the development of related industries.…”

Section: Introductionmentioning

confidence: 99%

Multiomics Reveals the Key Microorganisms and Metabolites in the Resistance to Root Rot Disease of Paris polyphylla

Ye,

Su,

Zheng

et al. 2023

Genes

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Root rot of Paris polyphylla has received widespread attention due to its threat to yield and leads to serious economic losses. However, the relationship among the rhizosphere microbial community, metabolites and root rot disease remained largely unexplored. Herein, we used integrated 16S rRNA, ITS, RNA sequencing and UPLC-MS/MS to systematically investigate the differences between healthy and diseased P. polyphylla. We found that root rot reduced the microbial diversity in the diseased P. polyphylla compared with the healthy control. The relative abundance of the bacterial phylum Actinobacteria increased in the diseased rhizome of P. polyphylla. For the fungal community, root rot disease contributed to an increased relative abundance of Ascomycota and decreased Glomeromycota at the phylum level. The transcriptomic results showed that the differently expressed genes were significantly enriched in the “Biosynthesis of various alkaloids”, “flavonoid biosynthesis” and “isoflavonoid biosynthesis” and “Phenylpropanoid biosynthesis” was dramatically enriched in healthy P. polyphylla compared with that in diseased P. polyphylla. Likewise, the metabolomic results showed that the biosynthesis of secondary metabolites and metabolic pathways was found to be significantly enriched by differential metabolites. Taken together, the study of combining metabolomics with microbiomes can help us enhance our understanding of the mechanisms of plant resistance to root rot disease, thereby discovering specific metabolites and microorganisms that can resist pathogen infection in P. polyphylla.

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Steroidal saponins and lignan glycosides from the rhizomes of Paris polyphylla var. latifolia

Cited by 4 publications

References 11 publications

Cytotoxic Steroidal Saponins Containing a Rare Fructosyl from the Rhizomes of Paris polyphylla var. latifolia

Cytotoxic Steroidal Saponins Containing a Rare Fructosyl from the Rhizomes of Paris polyphylla var. latifolia

A Simple and Efficient UPLC-ESI-Q-TOF-MS Method for Determining the Chemical Composition in Rhizomes of Multiple Stems Paris polyphylla var. yunnanensis

Multiomics Reveals the Key Microorganisms and Metabolites in the Resistance to Root Rot Disease of Paris polyphylla

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