Steroid metabolism in fish

Truscott, B.

doi:10.1016/0016-6480(83)90063-1

General and Comparative Endocrinology

1983

DOI: 10.1016/0016-6480(83)90063-1

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Steroid metabolism in fish

B. Truscott

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1985

2002

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Cited by 16 publications

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Biological measurement of estrogenic activity in urine and bile conjugates with the in vitro ER‐CALUX reporter gene assay

Legler

Jonas

Lahr³

et al. 2002

Enviro Toxic and Chemistry

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Although estrogens are excreted as biologically inactive conjugates, they can be reconverted to an active form, possibly by bacteria. A simple method was developed to deconjugate estrogen metabolites present in human urine and fish bile back to active estrogens by enzymatic hydrolysis with beta-glucuronidase or live Escherichia coli cells. Deconjugated extracts were tested for estrogenic activity in the in vitro stable estrogen receptor-mediated chemical-activated luciferase gene expression (ER-CALUX) assay. Estrogen glucuronides in urine obtained from human males and females were effectively converted to active forms after incubation with beta-glucuronidase or E. coli. The highest estrogenic activity was found in deconjugated metabolites from urine of a pregnant woman, in which levels up to 3,000 nmol estradiol equivalents per liter of urine were found after overnight incubation of urine with E. coli. Bile sampled from male bream and flounder from various freshwater and marine locations was also deconjugated and a good correlation was found between high biliary estrogenic activity and elevated levels of xenoestrogenic activity in surface water as well as in plasma vitellogenin. Therefore, the measurement of deconjugated bile could form a useful (indirect) biomarker for internal dose of xenoestrogens in male fish.

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Biological measurement of estrogenic activity in urine and bile conjugates with the in vitro ER‐CALUX reporter gene assay

Legler

Jonas

Lahr³

et al. 2002

Enviro Toxic and Chemistry

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Involvement of Multiple Biotransformation Processes in the Metabolic Elimination of Testosterone by Juvenile and Adult Fathead Minnows (Pimephales promelas)

Parks

LeBlanc

1998

General and Comparative Endocrinology

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Male priming pheromone is present in bile, as well as urine, of female rainbow trout

Scott

2001

Journal of Fish Biology

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The bile of sexually mature female rainbow trout Oncorhynchus mykiss has pheromonal activity which causes a significant increase in concentrations of 17,20 -dihydroxy-4-pregnen-3-one in the plasma of males. Bile from male trout is inactive. The activity in the female bile binds to octadecylsilane and can be eluted with methanol. The synthetic bile acids, taurocholic acid and taurolithocholic acid, are inactive.

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Steroid metabolism in fish

Cited by 16 publications

References 20 publications

Biological measurement of estrogenic activity in urine and bile conjugates with the in vitro ER‐CALUX reporter gene assay

Biological measurement of estrogenic activity in urine and bile conjugates with the in vitro ER‐CALUX reporter gene assay

Involvement of Multiple Biotransformation Processes in the Metabolic Elimination of Testosterone by Juvenile and Adult Fathead Minnows (Pimephales promelas)

Male priming pheromone is present in bile, as well as urine, of female rainbow trout

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