Steroid measurement with LC–MS/MS in pediatric endocrinology

Abstract

Please cite this article as: Rauh, M., Steroid measurement with LC-MS/MS in pediatric endocrinology, Molecular and Cellular Endocrinology (2008), doi:10.1016/j.mce.2008 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

LC-MS candidate reference methods for the harmonisation of parathyroid hormone (PTH) measurement: a review of recent developments and future considerations

“…Modern LC-MS instruments are highly sensitive, and coupled with appropriate sample preparation techniques, can often achieve limits of detection comparable with immunoassays (e.g., pmol/L for some analytes). However, it is the combination of chromatographic separation with a detection system based on mass-to-charge (m/z) ratio, which provides a specificity of LC-MS that is far superior to that of immunoassay-based detection [10,40,41].…”

Diagnostic Evaluation of Enzyme Activity Related to Steroid Metabolism by Mass Spectrometry-Based Steroid Profiling

“…25 In addition, it was also useful for the differential diagnosis of non-classical forms of CAH, which may not be distinguishable by clinical presentation. [25][26][27] Compared to MS profiling combined with chromatographic separation, matrix-assisted laser desorption ionization (MALDI)-MS has the advantage of high sample throughput, making it attractive for clinical applications. In addition, it has been successfully applied to measure 10 adrenal steroids, including 17-OHP, in the serum of CAH patients.…”

Increasing Liquid Chromatography–Tandem Mass Spectrometry Throughput by Mass Tagging: A Sample-Multiplexed High-Throughput Assay for 25-Hydroxyvitamin D2 and D3

“…The use of liquid chromatography-tandem mass spectrometry (LC-MS/MS) 3 has increased in recent years (1)(2)(3). LC-MS/MS is increasingly preferred over HPLC-UV or immunoassay for low molecular weight analytes because it is less susceptible to interfering cross-reactivity from structurally related compounds (4 -9 ), an issue particularly problematic at low analyte concentrations (10 -14 ).…”

“…The most obvious setbacks are manual workflows and limited throughput. The latter has become especially apparent in highvolume tests such as 25-hydroxyvitamin D 2 and D 3 [25(OH)D 2 and 25(OH)D 3 ], testosterone, and estradiol. Daily test volumes of hundreds of samples are not unusual for these analytes, with large referral laboratories often performing thousands of these tests each day.…”