Steroid‐Hormone‐Binding Proteins in Benign and Malignant Breast Tissue and Their Relevance for Prognosis

Etzrodt, A.; Jung, Hyun Cheol; Mundelius, B.; Rogge, Mark; Tolxdorff, Thomas

doi:10.1111/j.1749-6632.1986.tb16081.x

Cited by 1 publication

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“…Therefore, the cellular uptake of MIP-NGs depends on the HSA concentration in the medium. It was previously reported that the albumin concentration is lower in tumor tissues than in the bloodstream and that the albumin adsorption on MIP-NGs was dynamic and concentration-dependent. Therefore, it is likely that the cellular uptake of MIP-NGs was promoted after they penetrated tumor tissues because the cellular uptake was enhanced in response to decreasing albumin concentration.…”

Section: Resultsmentioning

confidence: 99%

Cellular Interaction Regulation by Protein Corona Control of Molecularly Imprinted Polymer Nanogels Using Intrinsic Proteins

Hayakawa

Yamada

Kitayama

et al. 2020

ACS Appl. Polym. Mater.

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Nanomaterials have a great potential for use in various biorelated applications such as drug delivery systems and in vivo imaging; understanding nanoparticle–cell interactions is an important requisite for these applications. Herein, the nanoparticle–cell interactions, including the cellular uptake mechanism, were investigated in detail using polymer nanogels possessing molecular recognition ability (molecularly imprinted polymer nanogels: MIP-NGs) capable of protein corona regulation via albumin recognition and using refractory cancer cell lines and an immune-related cell line. Albumin recognition in the MIP-NGs further increased the albumin-dependent inhibition of cellular uptake of the nanogels, including uptake by cancer cells and macrophages. Cellular uptake was inhibited more efficiently in MIP-NGs than in the reference nonimprinted polymer nanogels without albumin recognition cavities. In the presence of serum albumin, MIP-NGs did not cause a significant upregulation of inflammatory reactions as measured by cytokine secretion by macrophages. To the best of our knowledge, our study is the first to use molecular recognition of albumin in nanogels to regulate the protein corona and in turn control cellular interactions. Our results provide strong evidence that MIP-NGs could utilize human serum albumin in the formation of a protein corona to avoid immune responses. We hope our results can provide important insights that translate into biomedical applications of nanomaterials.

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Section: Resultsmentioning

confidence: 99%