Sternal Marrow of Children in Normal and in Pathologic States

Vogel, Peter; Bassen, Frank A.

doi:10.1001/archpedi.1939.01990020003001

Cited by 31 publications

(18 citation statements)

References 5 publications

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“…There is no loss of cytoplasm when tissue is sectioned; thus, the close agreement of our data with those obtained by Harrison supports his contention (Table VII). The data presented here on megakaryocytes are in agreement with some of the previous reports using marrow smear ( 13,14) and suspension ( 15) methods (Table VIII). Published studies employing section methods gave values four to five times greater than the present report (16,17) ; however, if appropriate corrections are made for "multiple counting" (based on the reported tissue section thickness), the results are in good agreement with the present study.…”

Section: Calculationssupporting

confidence: 93%

Megakaryocyte quantitation

Harker¹

1968

J. Clin. Invest.

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A method is described for the quantitation of megakaryocytes. In this technic bone marrow sections obtained at the time of maximal marrow uptake of previously injected plasma bound 59Fe are counted for radioactivity as well as the number of megakaryocytes and nucleated red cells. We calculated the total megakaryocytes by relating the ratio of megakaryocyte: radioactivity with the total marrow 59Fe activity at the time of sampling. A multiple counting correction factor which is related to cell diameter and section thickness is required for quantitation of cell numbers from section material. The normal megakaryocyte number for the rat is 11.0 x 106/kg, and 6.1 x 106/kg for man.

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Section: Calculationssupporting

confidence: 93%

Megakaryocyte quantitation

Harker¹

1968

J. Clin. Invest.

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“…They resemble the micromyeloblasts illustrated by N aegeli (25) and Israels (18), and the cells of acute stem-cell leukaemia in children illustrated by Blackfan and Diamond (2). They are reminiscent of the "hematogoncs" in the bone marrow of young children (19,31). There is no evidence that small lymphocytes give rise to these small pale cells.…”

Section: The Accumulation Of Small Mononuclear Cells In Irradiated Mamentioning

confidence: 95%

Observations on the Accumulation of Mononuclear Cells and the Activities of Reticulum Cells in Bone Marrow of Guinea-Pigs Recovering from Whole Body Gamma Irradiation

Harris¹,

Haigh²,

Kugler³

1963

Acta Haematol

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“…Hematogones (B-lymphocyte precursors), originally recognized by their morphologic features in bone marrow smears, 1,2 are found in small numbers in most marrow specimens analyzed by flow cytometry. They are reported to occur in large numbers in some healthy infants and young children and in a variety of diseases in both children and adults.…”

Section: Introductionmentioning

confidence: 99%

Immunophenotypic analysis of hematogones (B-lymphocyte precursors) in 662 consecutive bone marrow specimens by 4-color flow cytometry

McKenna

Washington

Aquino

et al. 2001

Blood

338

281

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Bone marrow hematogones (B-lymphocyte precursors) may cause problems in diagnosis because of their morphologic and immunophenotypic similarities to neoplastic lymphoblasts. The purposes of this prospective, multiparametric flow cytometry study were to quantify hematogones across age groups and a spectrum of clinical conditions, to identify factors that affect the relative quantity of hematogones, and to compare their immunophenotype with that of neoplastic lymphoblasts. A total of 662 consecutive marrow specimens were analyzed for hematogones using one of two 4-color antibody combinations; hematogones were identified in 528 (79.8%). There was a significant decline in hematogones with increasing age (P < .001), but a broad range was found at all ages and many adults had a relatively high number. Specimens processed by density gradient had a higher mean percent hematogones than those processed by erythrocyte lysis (P < .001). There was a direct decline in hematogones with increasing marrow involvement with neoplastic cells. A total of 8% of the 662 specimens contained 5% or more hematogones: 24.6% of specimens from patients aged less than 16 years and 6.3% from those 16 and older (P < .000 01).

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Sternal Marrow of Children in Normal and in Pathologic States

Cited by 31 publications

References 5 publications

Megakaryocyte quantitation

Megakaryocyte quantitation

Observations on the Accumulation of Mononuclear Cells and the Activities of Reticulum Cells in Bone Marrow of Guinea-Pigs Recovering from Whole Body Gamma Irradiation

Immunophenotypic analysis of hematogones (B-lymphocyte precursors) in 662 consecutive bone marrow specimens by 4-color flow cytometry

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