Megakaryocyte quantitation

Harker, L A

doi:10.1172/jci105741

Cited by 64 publications

(45 citation statements)

References 13 publications

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“…In one thin section, 233 discrete platelets were counted. Using 2.1 p.m as the thickness of these nondiscoid platelets, and Harker's calculation (18) for extrapolation from one section to the content of the entire ellipsoid, a total platelet count of 2,560 was estimated for this cell. This result is comparable to the protein content ratio (above), when one considers that the pool of isolated megakaryocytes comprised immature, developing, and fully mature cells, and that the latter alone would be expected to have a higher ratio of protein content (compared to platelets) than the isolated pool.…”

Section: Resultsmentioning

confidence: 99%

Origin of platelet-derived growth factor in megakaryocytes in guinea pigs.

Chernoff¹,

Levine²,

Goodman³

1980

J. Clin. Invest.

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Section: Resultsmentioning

confidence: 99%

Origin of platelet-derived growth factor in megakaryocytes in guinea pigs.

Chernoff¹,

Levine²,

Goodman³

1980

J. Clin. Invest.

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“…Some studies have focused on the use of flow cytometry or special handling such as saponin lysis with microfiltration of bone marrow aspirates to assess megakaryocyte content. 16,17 Thiele et al 18 examined bone marrow biopsies stained immunohistochemically with antibodies to CD 61 (gpIIIa). In addition to a group of control patients, megakaryocytes were evaluated in biopsies from patients who had reactive thrombocytosis and thrombocytopenia.…”

Section: Discussionmentioning

confidence: 99%

Prolonged isolated thrombocytopenia after hematopoietic stem cell transplantation: morphologic correlation

BIELSKI

Yomtovían

Lazarus

et al. 1998

Bone Marrow Transplant

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Summary:Prolonged isolated thrombocytopenia, defined as recovery of other cell counts with continuous dependence on platelet transfusions for greater than 90 days after hematopoietic stem cell transplantation (HSCT), develops in approximately 5% of patients who undergo HSCT. Although the clinical conditions associated with prolonged isolated thrombocytopenia have been studied, a systematic review of bone marrow biopsies has not been performed and the pathophysiologic basis has not been defined. We reviewed all HSCT at one center from 1990 to 1995 (n = 454) and found 12 cases that met criteria for prolonged isolated thrombocytopenia (incidence = 12/454 or 3%). Bone marrow core biopsies from 12 patients with prolonged isolated thrombocytopenia were reviewed to determine cellularity, numbers of megakaryocytes, the presence of atypical forms, and clusters of megakaryocytes. These marrow megakaryocyte counts were compared to age and disease matched controls, and 11 normal donors. Patients (aged 1-56 years, mean 32 years) who underwent HSCT (four sibling HLA-identical, five autologous bone marrow, three autologous peripheral stem cell) with prolonged isolated thrombocytopenia had a statistically significant lower absolute megakaryocyte count in bone marrow biopsies performed before transplantation and more than 30 days after transplantation compared to control patients (aged 4 months to 50 years, mean 31 years) who underwent HSCT (four sibling HLA-identical, four autologous bone marrow, four autologous peripheral stem cell) for similar conditions. No apparent differences were seen in size of megakaryocytes, nuclear-cytoplasmic ratios, or clustering of megakaryocytes. Overall marrow cellularities were similar in the three groups. These findings suggest that decreased differentiation of megakaryocytes from stem cells, rather than ineffective platelet production or peripheral destruction of platelets, causes prolonged isolated thrombocytopenia in HSCT patients. Low megakaryocyte counts prior to HSCT may be a useful prognostic indicator, as this feature was associated with the development of prolonged isolated thrombocytopenia.

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“…To determine total marrow cellularity, the specific activity of normoblasts in individual sections was related to the total skeletal activity (3,4). Radioactivity in marrow sections was counted in a low background gas flow beta counter (Lowbeta Counter, Beckman Instruments, Inc., Fullerton, Calif.) to a statistical error of less than 3%.…”

Section: Methodsmentioning

confidence: 99%

“…The coefficient of variation of N/E ratios calculated from 10,000 cells in sections was less than 10%. The size correction factor derived in these studies has been discussed elsewhere in reference to megakaryocyte quantitation (3) and depends on the uniformity of section thickness. In the present study differences in section thickness were monitored by radioactive labeling of the tissue, and the mean variation of sections was found to be ±8.7%…”

Section: Methodsmentioning

confidence: 99%

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Marrow erythroid and neutrophil cellularity in the dog.

Deubeleiss¹,

Dancey²,

Harker³

et al. 1975

J. Clin. Invest.

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A B S T R A C T This paper describes a method for determining the number of marrow erythroid and neutrophil cells in which the cellularity of marrow sections was related to that of the total marrow by radioiron dilution. Tissue sections were prepared from methacrylateembedded dog marrow biopsies, and neutrophils were identified by staining of their primary granules. After correction of direct section counts for multiple counting error, accurate neutrophil-erythroid ratios were established with a coefficient of variation of less than 10% when 104 cells were examined. An average neutrophil-erythroid ratio of 1.2 was found in six normal dogs.The total number of nucleated red cells in the dog was 5.48±0.78 X 109/kg (+1 SD), and the corresponding erythron iron turnover was 0.90±0.11 mg Fe/100 ml whole blood/day. The total number of marrow neutrophils, derived from the neutrophil-erythroid ratio, was 6.6±0.59 X 109 cells/kg, of which 1.4 were promyelocytes and mvelocytes, 2.3 were metamyelocytes and bands, and 3.0 were segmented neutrophils. Leukopheresis studies were carried out in six dogs to confirm the accuracy of these cellular measurements. 1\Iarrow counts showed a mean decrease of 22.7 X 109 cells or 35% of the postmitotic neutrophil pool, and it was calculated that 10.2 x 109 additional cells had been taken from already circulating blood. This estimated deficit of 32.9 X 109 was almost identical to the 33 X

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Megakaryocyte quantitation

Cited by 64 publications

References 13 publications

Origin of platelet-derived growth factor in megakaryocytes in guinea pigs.

Origin of platelet-derived growth factor in megakaryocytes in guinea pigs.

Prolonged isolated thrombocytopenia after hematopoietic stem cell transplantation: morphologic correlation

Marrow erythroid and neutrophil cellularity in the dog.

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