Sterilization of hydrogen peroxide resistant bacterial spores with stabilized chlorine dioxide

Friedline, Anthony; Zachariah, Malcolm M.; Middaugh, Amy; Heiser, Matt; Khanna, Neeraj; Vaishampayan, Parag; Rice, Charles V.

doi:10.1186/s13568-015-0109-4

Cited by 15 publications

(15 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Spore suspensions of Bacillus subtilis (ATCC 6633) similar to those used in clean-room sterilization effectiveness (Friedline et al, 2015) and bacteriostasis testing under Mars-like conditions (Kerney and Schuerger, 2011;Schuerger et al, 2017Schuerger et al, , 2003 were acquired from Crosstex (Part# SBS-08) to represent a worst case DNA extraction scenario of a tough-to-lyse organism. Spore suspension were DNAse-treated (New England Biolabs, M0303L) in order to remove any extracellular DNA and counted using a viable spore assay on lysogeny broth agar plates (Mojarro et al, 2017b).…”

Section: Bacillus Subtilis Sporesmentioning

confidence: 99%

Nucleic acid extraction and sequencing from low-biomass synthetic Mars analog soils for in situ life detection

Mojarro

Hachey

Bailey

et al. 2018

Preprint

View full text Add to dashboard Cite

Recent studies regarding the origin of life and Mars-Earth meteorite transfer simulations suggest that biological informational polymers, such as nucleic acids (DNA and RNA), have the potential to provide unambiguous evidence of life on Mars. To this end, we are developing a metagenomicsbased life-detection instrument which integrates nucleic acid extraction and nanopore sequencing:The Search for Extra-Terrestrial Genomes (SETG). Our goal is to isolate and sequence nucleic acids from extant or preserved life on Mars in order to determine if a particular genetic sequence (1) is distantly-related to life on Earth indicating a shared-ancestry due to lithological exchange, or (2) is unrelated to life on Earth suggesting a convergent origin of life on Mars. In this study, we validate prior work on nucleic acid extraction from cells deposited in Mars analog soils down to microbial concentrations observed in the driest and coldest regions on Earth. In addition, we report low-input nanopore sequencing results equivalent to 1 ppb life-detection sensitivity achieved by employing carrier sequencing, a method of sequencing sub-nanogram DNA in the background of a genomic carrier.

show abstract

Section: Bacillus Subtilis Sporesmentioning

confidence: 99%

Nucleic acid extraction and sequencing from low-biomass synthetic Mars analog soils for in situ life detection

Mojarro

Hachey

Bailey

et al. 2018

Preprint

View full text Add to dashboard Cite

show abstract

“…Kreske and others () reported that treatment with ClO 2 resulted in a more rapid reduction in B. cereus spore numbers compared to treatment with NaOCl. The antibacterial activity of ClO 2 at various concentrations against Bacillus spores has been reported (Kreske and others ; Kim and others ; Kim and others ; Patel and others ; Friedline and others ). Treatment with 24 or 48 μg/mL of ClO 2 for 1 min resulted in an approximately 1.5 log reduction in B .…”

Section: Resultsmentioning

confidence: 92%

“…subtilis spore numbers; the spores were completely inactivated by 2 min of treatment (Patel and others ). Bacillus pumilus and B. subtilis spores dried on glass cover slips were completely killed by treatment with 47 or 187 μg/mL ClO 2 for 24 h or 10 min, respectively (Friedline and others ); moreover, a greater number of spores were deactivated as ClO 2 concentration and exposure time increased. Kim and others () reported that treatment with organic acid‐based ClO 2 (100 μg/mL) for 10 min reduced numbers of B. cereus spores (6.5 log CFU/mL) below the limit of detection (0.3 log CFU/mL).…”

Section: Resultsmentioning

confidence: 99%

Inactivation of Bacillus cereus Spores on Red Chili Peppers Using a Combined Treatment of Aqueous Chlorine Dioxide and Hot‐Air Drying

Kim

Lee

Ryu

et al. 2017

Journal of Food Science

View full text Add to dashboard Cite

The effect of a combined treatment using aqueous chlorine dioxide (ClO ) and hot-air drying to inactivate Bacillus cereus spores on red chili peppers was evaluated. Ten washed and dried pepper samples, each comprising half of a single pepper (Capsicum annuum L.), were inoculated with B. cereus spore suspension. The inoculated samples were washed with sodium hypochlorite (NaOCl; 50, 100, or 200 μg/mL) or ClO (50, 100, or 200 μg/mL) solution for 1 min and then air-dried (25 ± 1 °C, 47 ± 1% relative humidity), which was followed by drying with hot air at 55 °C for up to 48 h. The spore populations on the samples were enumerated and their a and chromaticity values were measured. The spore numbers immediately after treatment with NaOCl and ClO were not significantly different. A more rapid reduction in spore numbers was observed in the samples treated with ClO than those treated with NaOCl during drying. A combined treatment of ClO and hot-air drying significantly reduced the spore populations to below the detection limit (1.7 log CFU/sample). B. cereus spores on chili peppers were successfully inactivated by washing with ClO solution followed by hot-air drying whereas the pepper color was maintained.

show abstract

“…A possible explanation for the enhanced resistance of B. pumilus SAFR-032 spores 37 may arise from structural differences, and differences in water chemistry, compared to that of spores from B. subtilis 1A578 and ATCC 6051, previously reported. 29,37 Using scanning electron microscopy, it possible to identify an exosporium layer around B. pumilus SAFR-032 spores ( Figure 10A) that is not present around spores of B. subtilis 1A578 ( Figure 10B). It has been reported that the B. pumilus exosporium is composed of proteins that differ, and are in higher abundance, than the proteins within the exosporium of B. cereus and other species.…”

Section: Discussionmentioning

confidence: 99%

Sterilization Resistance of Bacterial Spores Explained with Water Chemistry

et al. 2015

View full text Add to dashboard Cite

Bacterial spores can survive for long periods without nutrients and in harsh environmental conditions. This survival is influenced by the structure of the spore, the presence of protective compounds, and water retention. These compounds, and the physical state of water in particular, allow some species of bacterial spores to survive sterilization schemes with hydrogen peroxide and UV light. The chemical nature of the spore core and its water has been a subject of some contention and the chemical environment of the water impacts resistance paradigms. Either the spore has a glassy core, where water is immobilized along with other core components, or the core is gel-like with mobile water diffusion. These properties affect the movement of peroxide and radical species, and hence resistance. Deuterium solid-state NMR experiments are useful for examining the nature of the water inside the spore. Previous work in our lab with spores of Bacillus subtilis indicate that, for spores, the core water is in a more immobilized state than expected for the gel-like core theory, suggesting a glassy core environment. Here, we report deuterium solid-state NMR observations of the water within UV- and peroxide-resistant spores from Bacillus pumilus SAFR-032. Variable-temperature NMR experiments indicate no change in the line shape after heating to 50 °C, but an overall decrease in signal after heating to 100 °C. These results show glass-like core dynamics within B. pumilus SAFR-032 that may be the potential source of its known UV-resistance properties. The observed NMR traits can be attributed to the presence of an exosporium containing additional labile deuterons that can aid in the deactivation of sterilizing agents.

show abstract

Sterilization of hydrogen peroxide resistant bacterial spores with stabilized chlorine dioxide

Cited by 15 publications

References 53 publications

Nucleic acid extraction and sequencing from low-biomass synthetic Mars analog soils for in situ life detection

Nucleic acid extraction and sequencing from low-biomass synthetic Mars analog soils for in situ life detection

Inactivation of Bacillus cereus Spores on Red Chili Peppers Using a Combined Treatment of Aqueous Chlorine Dioxide and Hot‐Air Drying

Sterilization Resistance of Bacterial Spores Explained with Water Chemistry

Contact Info

Product

Resources

About