Sterilization of Bacillus pumilus spores using supercritical fluid carbon dioxide containing various modifier solutions

Protection of Bacillus pumilus Spores by Catalases

“…The SAFR-032 genome was sequenced, and numerous protein-encoding genes were identified as putative candidates responsible for the strain's enhanced resistance to the various sterilization treatments tested (21). Understanding its hydrogen peroxide resistance is particularly important since H 2 O 2 is used in current and newly developed sterilization methods (29,46). So far, the major role in peroxide resistance has been attributed to small acid-soluble proteins associated with DNA (42).…”

Terminal sterilization: Conventional methods versus emerging cold atmospheric pressure plasma technology for non‐viable biological tissues

“…[62] Other chemical sterilization methods, occasionally used for TBTM; are rarely regarded as terminal sterilization due to technical, safety or biocompatibility barriers. Super critical carbon dioxide (SCCO 2 ) [63] , characterized by its efficacy against spores, penetration and biomaterial compatibility, [63][64][65][66] is reported to cause damage to materials, leaves residues and is ineffective against endotoxins.…”

Effect of High-pressure CO2Processing on Bacterial Spores

“…The addition of even a low concentration of a strong oxidant, such as hydrogen peroxide, tert-butyl hydroperoxide, peracetic acid or trifluoroacetic acid, to CO 2 could achieve high-efficacy inactivation of bacterial spores at mild temperatures (35-60ºC) (White et al, 2006;Zhang et al, 2006a;Zhang et al, 2006b;Zhang et al, 2007;Hemmer et al, 2007;Tarafa et al, 2009;Shieh et al, 2009;Checinska et al, 2011 reduction in the number of B. atrophaeus spores by HPCD treatment at 10 MPa and 35-41ºC for 27 min with the addition of 55 ppm PAA to CO 2 . All of these studies indicated that the addition of strong oxidants to CO 2 could result in a high inactivation ratio of bacterial spores at a lower temperature with a shorter treatment time.…”

“…In recent years, the number of published journal articles related to bacterial spore inactivation by HPCD treatment has significantly increased, reaching a cumulative number of 34 published journal articles in June 2012 ( Figure 1). In 27 of these published articles, 12 species of bacterial spores have been investigated: Bacillus subtilis (Kamihira et al, 1987;Ishikawa et al, 1997;Ballestra and Cuq., 1998;Spilimbergo et al, 2002;Spilimbergo et al, 2003a;Watanabe et al, 2003a;Karajanagi et al, 2011), B. cereus (Ishikawa et al, 1997;Spilimbergo et al, 2003a;Watanabe et al, 2003a;Garcia-Gonzalez et al, 2009), B. megaterium (Ishikawa et al, 1997;Enomoto et al, 1997), B. polymyxa (Ishikawa et al, 1997), B. coagulans (Ishikawa et al, 1997;Furukawa et al, 2003;Watanabe et al, 2003a;Watanabe et al, 2003b;Furukawa et al, 2004;Furukawa et al, 2006), B. licheniformis (Furukawa et al, 2003;Watanabe et al, 2003a;Watanabe et al, 2003b;Furukawa et al, 2004;Furukawa et al, 2006), B. pumilus Tarafa et al, 2009;Shieh et al, 2009;Checinska et al, 2011), B. atrophaeus (Hemmer et al, 2006;Zhang et al, 2006b;Qiu et al, 2009), B. anthracis (Zhang et al, 2007), Geobacillus stearothermophilus (Kamihira et al, 1987;Watanabe et al, 2003a;White et al, 2006;Hemmer …”