Sterilisation of skin allograft with gamma irradiation

Rooney, Paul; Eagle, M. J.; Hogg, Penny; Lomas, Richard; Kearney, J. N.

doi:10.1016/j.burns.2007.08.021

Cited by 38 publications

(40 citation statements)

References 16 publications

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“…Theoretically, GI-sterilized materials may be weaker already from the onset as irradiation causes structural changes in the col- lagen [13,14,22] . Our experiments did not show such differences between GI-and ETO-sterilized products after integration into the host, with one small caution that we did not perform tensiometry prior to implantation.…”

Section: Discussionmentioning

confidence: 99%

Porous Acellular Porcine Dermal Collagen Implants to Repair Fascial Defects in a Rat Model: Biomechanical Evaluation up to 180 Days

Ozog¹,

Konstantinovic²,

Zheng³

et al. 2009

Gynecol Obstet Invest

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Aim: To investigate the biomechanical properties of porous collagen matrices in a rat abdominal wall defect model. Study Design: 112 rats were implanted with non-cross-linked InteXèn LP, cross-linked Pelvicol, and two investigational acellular collagen matrices (ACMs) sterilized either with ethylene oxide (ACM ETO) or γ-irradiation (ACM GI). After 14, 30, 90 and 180 days, 7 animals per group were sacrificed to document adhesions, herniation, infection, stress resistance and histology. Results: The 2 sterilization methods did not cause measurable differences between ACMs. Pelvicol was more resistant than ACMs but showed degradation at 90 days without loss of strength. InteXèn LP became remodeled as a thin fibrous scar and was more resistant at all time points; however, some animals developed bulging. Conclusions: Non-cross-linked InteXèn LP became remodeled by 180 days with remarkable stress resistance. Despite cross-linking Pelvicol showed degradation. Comparable but investigational ACM explants were less resistant without morphologic differences to explain this.

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Section: Discussionmentioning

confidence: 99%

Porous Acellular Porcine Dermal Collagen Implants to Repair Fascial Defects in a Rat Model: Biomechanical Evaluation up to 180 Days

Ozog¹,

Konstantinovic²,

Zheng³

et al. 2009

Gynecol Obstet Invest

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“…Rooney et al found that irradiation with 25 kGy of deep-frozen skin soaked in low glycerol concentration (i.e., 20%) prior to irradiation is not harmful to tissue structure. 63 Lyophilization/freeze-drying Lyophilization (freeze-drying or cryodesiccation) is a dehydration process involving two consecutive steps: a freezing phase and a sublimation phase, in which low pressure allows frozen water to sublimate directly from a solid to a gaseous state. This processing technique is generally applied to dermal matrices.…”

Section: Gamma-irradiationmentioning

confidence: 99%

“…58 Briefly, skin is de-epidermized and processed for decellularization, which commonly consists of physical, biological (enzymatic) and chemical methods, variously combined. 41,43,[56][57][58][59][60][61][62][63][64][65] Such freeze-dried acellular dermis lacks immunogenic potential and is sterile, and can therefore be used as a dermal scaffold that integrates into the host tissue or closed wounds. It is easily stored and distributed, and is used when required after re-hydration (for 30−60 min at +37°C in sterile saline in the case of lyophilized dermis).…”

Section: Gamma-irradiationmentioning

confidence: 99%

Current insights into skin banking: storage, preservation and clinical importance of skin allografts

Tognetti¹,

Pianigiani²,

Ierardi³

et al. 2017

BSAM

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Donor skin and dermal grafts are used in several types of loss of substance for different clinical purposes. As biological physiological medication, donor skin grafts can promote re-epithelization, shorten healing time, alleviate pain and protect dermal and subcutaneous structures such as cartilage, tendons, bones and nerves. Though a great variety of dermal matrices and skin equivalents, both synthetic and semisynthetic, are now available for wound treatment, viable human skin allografts remain an important therapeutic choice for extensive deep burns and hard-to-heal wounds. In such cases, viable skin allografts have significantly better clinical outcomes than unviable human-derived allografts or synthetic medications. The demand for human-derived skin bioproducts continues to be a reason for the existence of skin banks. Skin bank organization is complex and requires continuous updating. Careful donor selection, thorough microbiological and serological donor screening for transmissible diseases and rigorous quality control during tissue preparation are necessary to minimize the risk of transmission of pathogenic agents. Skin banks must also observe standardized reproducible procedures to ensure tissue traceability and biological safety in all phases of processing and to avoid new biological contamination. Constant training and periodic checks are needed to keep skin bank operators attentive and responsible. Finally, skin banks should guarantee collection and storage of highly viable skin. Here, we discuss available tissue storage methods and the different types of skin bioproducts.

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“…Cryopreservation of skin is not expected to have an effect on the viability of C. burnetii in cutaneous tissues. Irradiation of cryopreserved skin has been shown to have a sterilizing effect on donor skin although the effect of irradiation on C. burnetii has not been tested specifically [46]. Thus, during processing and storage of cutaneous tissues, C. burnetii infection would probably not be detected, and whether it will be affected by it is insufficiently known.…”

Section: Musculoskeletal Tissuesmentioning

confidence: 99%

A Q Fever Outbreak in the Netherlands: Consequences for Tissue Banking

Wijk¹,

Hogema

Maas³

et al. 2011

Transfus Med Hemother

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Background: Emerging infectious diseases can compromise the safety of tissues for transplantations. A recent outbreak of Q fever, a zoonosis caused by the bacterium Coxiella burnetii, in the Netherlands compelled the Dutch tissue banks to assess the risk of Q fever transmission through tissue transplantation in order to maintain optimal safety. Methods: This article describes the systematic approach that was followed in the Netherlands. This approach included a review of the literature, a qualitative risk assessment, expert opinion gathering and investigations for specific strategies that can help to maintain the balance between tissue safety and availability. Results: This resulted in a specific donor selection policy and in development of further research to fill in gaps in knowledge about Q fever in tissue transplantation. Conclusion: The strategy described in this article may be useful for tissue bankers facing similar outbreaks of emerging infections or may be useful for development of future guidelines or assessment strategies for tissue banking.

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Sterilisation of skin allograft with gamma irradiation

Cited by 38 publications

References 16 publications

Porous Acellular Porcine Dermal Collagen Implants to Repair Fascial Defects in a Rat Model: Biomechanical Evaluation up to 180 Days

Porous Acellular Porcine Dermal Collagen Implants to Repair Fascial Defects in a Rat Model: Biomechanical Evaluation up to 180 Days

Current insights into skin banking: storage, preservation and clinical importance of skin allografts

A Q Fever Outbreak in the Netherlands: Consequences for Tissue Banking

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