Stereoselective analysis of carvedilol in human plasma using HPLC/MS/MS after chiral derivatization

Yang, Eric; Wang, Sherry; Kratz, John; Cyronak, Matthew J.

doi:10.1016/j.jpba.2004.07.008

Cited by 64 publications

(43 citation statements)

References 16 publications

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“…Similarly, carvidol [20] and gemifloxacin [24] enantiomers have been successfully analyzed after a simple protein precipitation. In the case of gemifloxacin [24], a new antibiotic, Ramji et al monitor both enantiomers and their metabolites after oral and intravenous injection in rat and dogs.…”

Section: Plasma and Serum Samplementioning

confidence: 99%

Liquid separation techniques coupled with mass spectrometry for chiral analysis of pharmaceuticals compounds and their metabolites in biological fluids

Erny

Cifuentes

2006

Journal of Pharmaceutical and Biomedical Analysis

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Determination of the chiral composition of drugs is nowadays a key step in order to determine purity, activity, bioavailability, biodegradation, etc, of pharmaceuticals. In this manuscript, works published for the last 5 years on the analysis of chiral drugs by liquid separation techniques coupled with mass spectrometry are reviewed. Namely, chiral analysis of pharmaceuticals including e.g., antiinflammatories, antihypertensives, relaxants, etc, by liquid chromatography-mass spectrometry and capillary electrophoresis-mass spectrometry are included. The importance and interest of the analysis of the enantiomers of the active compound and its metabolites in different biological fluids (plasma, urine, cerebrospinal fluid, etc) are also discussed.

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Section: Plasma and Serum Samplementioning

confidence: 99%

Liquid separation techniques coupled with mass spectrometry for chiral analysis of pharmaceuticals compounds and their metabolites in biological fluids

Erny

Cifuentes

2006

Journal of Pharmaceutical and Biomedical Analysis

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“…Carvedilol has been determined in biological fluids using highperformance liquid chromatography (HPLC) with fluorescence 1-7 , electrochemical detection 8 or mass spectrometry (MS) MS detection 13 . Reverse-phase (RP)-HPLC methods were described for the analysis of Carvedilol racemate 1, 3-5, 8, 9 or carvedilol enantiomers using chiral derivatization 2,10 . HPLC methods using a chiral stationary phase have been reported for the analysis of carvedilol enantiomers 6,7 .…”

Section: Introductionmentioning

confidence: 99%

“…HPLC methods using a chiral stationary phase have been reported for the analysis of carvedilol enantiomers 6,7 . The clean-up procedures for the extraction of carvedilol from biological matrix consist of protein precipitation 5,6,10,12 , solid-phase extraction (SPE) 2,3,9 , liquid-liquid extraction (LLE) 6,7,[11][12][13] , combinations of protein precipitation with SPE 4 or combinations of LLE with back-extraction 1,8 . Those methods use a large amount of biological samples (0.15-1.0 mL plasma or 2-5 mL urine samples) in order to obtain the high sensitivity or include time-consuming extraction procedures and/or relatively long run time.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Carvedilol and Its Metabolite in Human Plasma Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Janjanam¹,

Bimireddy²

2017

IJPTR

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: A rapid, sensitive and selective method for the determination of Carvedilol and its metabolite in human plasma was developed using liquid chromatography with tandem mass spectrometry (HPLC-MS/MS). Carvedilol, its metabolite 4-OH carvedilol and abacavir (internal standard) were extracted from human plasma by solid phase extraction technique (SPE) and analyzed on an Discovery column (C8, 50 × 4.6 mm, 5µ) with the mobile phase of acetonitrile -0.1% formic acid in water (70:30 v/v). The analytes were detected using an electro spray ionization tandem mass spectrometry in the multiple reaction monitoring mode. The standard curve was linear (r = 0.9998) over the concentration range of 0.5-100 ng/mL and 0.3-40 ng/mL for carvedilol and 4-OH carvedilol respectively. The lower limit of quantification for carvedilol was 0.5 ng/mL and 0.3 ng/mL for carvedilol and 4-OH carvedilol respectively using 500 µL plasma samples. The coefficient of variation and relative error for intra-and inter-assay at four QC levels were 3.37 to 9.53 %, 4.76 to 7.01% and 3.31 to 6.91%, 5.23 to 6.64 % respectively. The matrix effect for carvedilol, 4-OH carvedilol and abacavir were practically absent. The extraction recoveries of carvedilol, 4-OH carvedilol and abacavir were 78.90, 83.25 and 85.20%, respectively.

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“…Carvedilol has been quantified in biological fluids using high-performance liquid chromatography (HPLC) with fluorescence [1][2][6][7][8][9][10][11] or electrochemical detector [12], mass spectrometry [13][14][15], hydrophilic interaction liquid chromatography (HILIC) with tandem mass spectrometry [16], capillary electrophoresis-ultra violet detector [17] and gas chromatography (GC)-MS detector [18]. The clean-up procedures for the extraction of carvedilol from biological matrix has been carried out by protein precipitation [8][9]13,15], solid-phase extraction (SPE) [2,6,12], liquid-liquid extraction (LLE) [10,14,18], combinations of protein precipitation with SPE [7] or combinations of LLE with back-extraction [1,11].…”

Section: Introductionmentioning

confidence: 99%

“…The clean-up procedures for the extraction of carvedilol from biological matrix has been carried out by protein precipitation [8][9]13,15], solid-phase extraction (SPE) [2,6,12], liquid-liquid extraction (LLE) [10,14,18], combinations of protein precipitation with SPE [7] or combinations of LLE with back-extraction [1,11]. However, these methods used a large volume of biological fluids (0.15-1.0 ml plasma or 2-5 ml urine), time-consuming pre-treat procedures for LLE, SPE or derivatization steps and required a long run-time (20 min) to obtain high sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Rapid and Sensitive Carvedilol Assay in Human Plasma Using a High-Performance Liquid Chromatography with Mass/Mass Spectrometer Detection Employed for a Bioequivalence Study

Kim¹,

Lee²

2010

AJAC

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A method for the determination of carvedilol in human plasma was developed using a high-performance liquid chromatography with tandem mass spectrometer (HPLC-MS/MS). Plasma samples were deproteinized using acetonitrile and the supernatant was directly injected onto the HPLC column without any preparative steps. Chromatography was performed on a reversed-phase (C 18 ) column with isocratic mobile phase for 2 min. The calibration curve was linear over the range of 2 to100 ng/ml (R 2 > 0.9998) and the lower limit of quantitation (LLOQ) was 2 ng/ml. This method showed acceptable precision and accuracy, good recovery from the plasma matrix, and stability during the analytical procedures. When its application to the bioequivalence test of two carvedilol 25 mg tablet formulations in male healthy 28 volunteers, this validated analysis method was appropriate resulting in the bioequivalence of two formulations: no statistically significant difference was observed between the logarithmic transformed area under curve (AUC) and maximum plasma concentration (C max ) values of the two formulations. The 90% confidence interval for the ratio of the above mentioned two parameters were within the bioequivalence limit of 0.80-1.25. These results suggested that the HPLC-MS/MS analysis method developed was suitable for the carvedilol analysis in human plasma.

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Stereoselective analysis of carvedilol in human plasma using HPLC/MS/MS after chiral derivatization

Cited by 64 publications

References 16 publications

Liquid separation techniques coupled with mass spectrometry for chiral analysis of pharmaceuticals compounds and their metabolites in biological fluids

Liquid separation techniques coupled with mass spectrometry for chiral analysis of pharmaceuticals compounds and their metabolites in biological fluids

Analysis of Carvedilol and Its Metabolite in Human Plasma Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Rapid and Sensitive Carvedilol Assay in Human Plasma Using a High-Performance Liquid Chromatography with Mass/Mass Spectrometer Detection Employed for a Bioequivalence Study

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