Stereological analysis of acute lung injury

Ochs, Matthias

doi:10.1183/09059180.00010102

Cited by 21 publications

(22 citation statements)

References 73 publications

(83 reference statements)

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“…Design-based stereological methods were used to analyse the lung samples and obtain the quantitative data [27,28,34].…”

Section: Stereological Analysismentioning

confidence: 99%

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Pre-ischaemic exogenous surfactant reduces pulmonary injury in rat ischaemia/reperfusion

Mühlfeld¹,

Schaefer²,

Becker³

et al. 2008

European Respiratory Journal

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The optimal timing of exogenous surfactant application to reduce pulmonary injury and dysfunction was investigated in a rat lung ischaemia and reperfusion injury model.Lungs were subjected to flush perfusion, surfactant instillation, cold ischaemia (4uC, 4 h) and reperfusion (60 min). Animals received surfactant before (group 1) or at the end (2) of ischaemia, or during reperfusion (3) or not at all (4). Control groups included ''worst case'' without Perfadex and surfactant (5), ''no injury'' without (6) or with surfactant (7), and ischaemia with pre-ischaemic surfactant (8). Intra-alveolar oedema and blood-air barrier injury were estimated by light and electron microscopic stereology. Perfusate oxygenation and pulmonary arterial pressure (Ppa) were determined during reperfusion in groups 1 to 4.Intra-alveolar oedema was almost absent in groups 1, 6, 7 and 8, pronounced in 2, 3 and 4, and severe in 5. Blood-air barrier injury was moderate in groups 1 and 8, slightly pronounced in 2, 3 and 4, extensive in 5 and almost absent in 6 and 7. Perfusate oxygenation was significantly higher in group 1 compared with groups 2 to 4. Ppa did not differ between the groups.In conclusion, exogenous surfactant attenuates intra-alveolar oedema formation and blood-air barrier damage and improves perfusate oxygenation in the rat lung, especially when applied before ischaemic storage.

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“…Design-based stereological methods were used to analyse the lung samples and obtain the quantitative data [27,28,34].…”

Section: Stereological Analysismentioning

confidence: 99%

“…Using an extracorporeal I/R injury rat lung model, a combined light and electron microscopic approach and design-based stereology [27,28], the extent to which intra-alveolar oedema and injury of the blood-air barrier occurred was investigated.…”

mentioning

confidence: 99%

Pre-ischaemic exogenous surfactant reduces pulmonary injury in rat ischaemia/reperfusion

Mühlfeld¹,

Schaefer²,

Becker³

et al. 2008

European Respiratory Journal

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“…Recognising that most of the scientists in pulmonary research (including ourselves) are thinking in terms of disease-related studies, both in clinical studies and in studies of animal models, the aim of the present issue of the ERR is to first give a general introduction into designbased stereology [12] and then to demonstrate how these tools have already contributed or could potentially contribute to the understanding of a specific disease, i.e. acute lung injury [23], allergic bronchial asthma [24] and pulmonary emphysema [25], by studying appropriate animal models. Realising that we could not adequately cover some areas that significantly rely on quantitative morphology, we were happy that Connie C.W.…”

Section: Introductionmentioning

confidence: 99%

There is no rationale to still rely on outdated, biased tools for quantitative morphology in pulmonary research

Fehrenbach¹

2006

European Respiratory Review

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“…This is particularly suitable for the lung, as it can be applied to structures that are inhomogeneous or cannot be assumed to be homogeneous. Despite this and the subsequent developments made towards obtaining unbiased quantitative data of inhomogeneous structures, such as lung parenchyma and the bronchial tree, stereology has been used less in pulmonary research than in other fields.In recent years, the European Respiratory Society (ERS) has organised two courses dedicated to quantitative morphology in pulmonary research, followed by a series of articles in one issue of the European Respiratory Review which offered insight into the theoretical principles and practical applications of design-based stereology to graduate students and scientists [7][8][9][10][11][12][13]. …”

mentioning

confidence: 99%

“…In recent years, the European Respiratory Society (ERS) has organised two courses dedicated to quantitative morphology in pulmonary research, followed by a series of articles in one issue of the European Respiratory Review which offered insight into the theoretical principles and practical applications of design-based stereology to graduate students and scientists [7][8][9][10][11][12][13].…”

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confidence: 99%

Stereology: a bridge to a better understanding of lung structure and function

Brusasco¹,

Dinh-Xuan²

2010

European Respiratory Journal

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U nlike solid organs, the lung is a hollow organ that contains, under physiological conditions, an amount of air that is much larger than the total amount of its tissue. This confers to the lung a degree of heterogeneity that is much larger than in any other organ and adds difficulties to the interpretation of physiological measurements, which are in most cases related to the whole system. To overcome these difficulties and to construct suitable models for lung function, a number of studies on the relationships between structure and function have been performed over the last five decades. The major problem in relating lung function measurements to morphological data is the need to gather information on a three-dimensional (3D) structure from two-dimensional (2D) observations. Although data from simple 2D images have been widely used to describe pathological changes in lung parenchyma or airways, their relevance to physiological events occurring in 3D structures is limited.The key to obtaining valuable 3D morphometric data from 2D measurements is provided by stereology. This method had its foundations lain in 1777 by G. Buffon, who discovered the relationships between geometry and probability [1]. This opened the way for classical model-based stereology, which required an assumption of homogeneity of 3D structure. Stereology was first applied to medicine in the 1940s and to respiratory systems in 1952 by CAMPBELL and TOMKEIEFF [2], who calculated the internal surface of the lung.Major advances in lung quantitative morphometry were bestowed by WEIBEL and co-workers [3][4][5][6] between the 1960s and 70s. They provided the basic tools for modern designbased stereology. This is particularly suitable for the lung, as it can be applied to structures that are inhomogeneous or cannot be assumed to be homogeneous. Despite this and the subsequent developments made towards obtaining unbiased quantitative data of inhomogeneous structures, such as lung parenchyma and the bronchial tree, stereology has been used less in pulmonary research than in other fields.In recent years, the European Respiratory Society (ERS) has organised two courses dedicated to quantitative morphology in pulmonary research, followed by a series of articles in one issue of the European Respiratory Review which offered insight into the theoretical principles and practical applications of design-based stereology to graduate students and scientists [7][8][9][10][11][12][13].

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Stereological analysis of acute lung injury

Cited by 21 publications

References 73 publications

Pre-ischaemic exogenous surfactant reduces pulmonary injury in rat ischaemia/reperfusion

Pre-ischaemic exogenous surfactant reduces pulmonary injury in rat ischaemia/reperfusion

There is no rationale to still rely on outdated, biased tools for quantitative morphology in pulmonary research

Stereology: a bridge to a better understanding of lung structure and function

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