Step-wise control of protein adsorption and bacterial attachment on a nanowire array surface: tuning surface wettability by salt concentration

Wang, Lei; Wang, Hongwei; Lin, Yuan; Yang, Weikang; Wu, Zhaoqiang; Chen, Hong

doi:10.1039/c1jm12148k

Cited by 46 publications

(46 citation statements)

References 35 publications

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“…41 The ionic strength-responsive wettability can be further used to control bacterial attachment, because type I pilus for bacterial attachment interacts strongly with hydrophobic surfaces but is resisted by hydrophilic surfaces, increasing NaCl concentrations resulted in decreased attachment of Escherichia coli bacteria on the surface. For example, SiNWAs modied with the ionic strength-responsive polymer poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) exhibited responsive surface wettability between highly hydrophobic and hydrophilic values with increasing or decreasing NaCl concentrations.…”

Section: Effects Of Nanostructure On Cellular Behaviorsmentioning

confidence: 99%

Vertical SiNWAs for biomedical and biotechnology applications

Liu

2014

J. Mater. Chem. B

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Vertical silicon nanowire arrays (SiNWAs) are considered as one of the most promising nanomaterials. Notably, silicon-based nanomaterials exhibit excellent biocompatibility, and the diameters of silicon nanowires are comparable to the dimensions of many biological molecules, providing SiNWAs with great potential for life science applications. In this review, we first briefly introduce the synthesis, patterning and surface functionalization of SiNWAs and then focus on the recent progress in the application of SiNWAs for biosensors, studies on mammalian cells or bacteria with nanomaterials, controlled capture/ adsorption and release of cells or proteins, drug delivery, DNA transformation, antifouling surfaces, and nanozyme. We conclude with a brief perspective on future research directions and on the major challenges in this promising field. Fig. 7 (a) Schematic illustration of anti-EpCAM-coated SiNWA substrate for the enhanced capture of CTCs; (b) schematic illustration of a NanoVelcro chip with SiNWAs integrated in a PDMS fluidic device for capturing CTCs; (c) schematic illustration of an aptamer-coated Nano-Velcro Chip for capturing and releasing CTCs upon enzymatic treatment; (d) schematic illustration of DNA-coated SiNWAs for capturing and releasing T cells (top) and fluorescence microscopy images of captured cells before and after treatment with exonuclease (bottom); (e) and (f) schematic illustration of PNIPAAm-based polymer-coated SiNWAs for capturing and releasing CTCs in response to temperature; (g) schematic illustration of PAAPBA-coated SiNWAs for capturing and releasing CTCs in response to pH and glucose. 91 [Reprinted with permission of ref. 84,

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Section: Effects Of Nanostructure On Cellular Behaviorsmentioning

confidence: 99%

Vertical SiNWAs for biomedical and biotechnology applications

Liu

2014

J. Mater. Chem. B

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“…[18][19][20][21][22][23] Nanostructure spacing on the surface is a key issue, particularly when the characteristic dimensions of the array are in the same scale of the cells. 22 Nanopillar 22,23 and nanowire arrays [24][25][26][27] modulate bacterial motility, cell angular orientation upon adhesion, and cell replication, surpassing the importance of other microenvironmental parameters. [25][26][27] However, the molecular mechanisms of bacterial cell adhesion, which are triggered by nanotopography, have not been fully addressed yet.…”

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confidence: 98%

“…22 Nanopillar 22,23 and nanowire arrays [24][25][26][27] modulate bacterial motility, cell angular orientation upon adhesion, and cell replication, surpassing the importance of other microenvironmental parameters. [25][26][27] However, the molecular mechanisms of bacterial cell adhesion, which are triggered by nanotopography, have not been fully addressed yet. Accurate investigation of such interplay could be achieved by quantitatively measuring and probing the adhesion forces of cells.…”

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confidence: 98%

Nanowire Arrays as Cell Force Sensors To Investigate Adhesin-Enhanced Holdfast of Single Cell Bacteria and Biofilm Stability

et al. 2016

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Surface attachment of a planktonic bacteria, mediated by adhesins and extracellular polymeric substances (EPS), is a crucial step for biofilm formation. Some pathogens can modulate cell adhesiveness, impacting host colonization and virulence. A framework able to quantify cell-surface interaction forces and their dependence on chemical surface composition may unveil adhesiveness control mechanisms as new targets for intervention and disease control. Here we employed InP nanowire arrays to dissect factors involved in the early stage biofilm formation of the phytopathogen Xylella fastidiosa. Ex vivo experiments demonstrate single-cell adhesion forces up to 45 nN, depending on the cell orientation with respect to the surface. Larger adhesion forces occur at the cell poles; secreted EPS layers and filaments provide additional mechanical support. Significant adhesion force enhancements were observed for single cells anchoring a biofilm and particularly on XadA1 adhesin-coated surfaces, evidencing molecular mechanisms developed by bacterial pathogens to create a stronger holdfast to specific host tissues.

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“…In previous studies, electrostatic interactions between nanoparticles and proteins were fully disrupted at quite low salt concentrations, typically 10–50 mM salt. 25 In contrast, high binding affinities were observed between AuNP-COOH and both 12×His-GFP ( K s = 1.3 ±0.16 × 10 7 M −1 ), and 6×His-GFP ( K s = 1.4 ±0.2 × 10 6 M −1 ) in PBS buffer (150 mM NaCl in 5 mM PB, pH 7.4) (Fig. 2b).…”

Section: Resultsmentioning

confidence: 87%

Environmentally responsive histidine–carboxylate zipper formation between proteins and nanoparticles

et al. 2014

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Interfacing synthetic materials with biomacromolecules provides new systems for biological applications. We report the creation of a reversible multivalent supramolecular "zipper" recognition motif between gold nanoparticles and proteins. In this assembly, carboxylate-functionalized nanoparticles interact strongly with oligohistidine tags. This interaction can be tuned through His-tag length, and offers unique binding profiles based on the pH and electrolyte concentration of the medium.

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Step-wise control of protein adsorption and bacterial attachment on a nanowire array surface: tuning surface wettability by salt concentration

Cited by 46 publications

References 35 publications

Vertical SiNWAs for biomedical and biotechnology applications

Vertical SiNWAs for biomedical and biotechnology applications

Nanowire Arrays as Cell Force Sensors To Investigate Adhesin-Enhanced Holdfast of Single Cell Bacteria and Biofilm Stability

Environmentally responsive histidine–carboxylate zipper formation between proteins and nanoparticles

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