Stem cells of the lamina propria of human oral mucosa and gingiva develop into mineralized tissues in vivo

Treves-Manusevitz, Sandra; Hoz, Leticia Rosales; Rachima, Heled; Montoya, G.; Tzur, Ephraim; Vardimon, Alexander D.; Narayanan, A. Sampath; Amar, Salomon; Arzate, Higinio; Pitaru, S.

doi:10.1111/jcpe.12016

Cited by 38 publications

(46 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, after release from their niche the remote microenvironment containing cementum attachment protein appears to influence these cells to form a mineralized-like structure and express cementoblastic /osteoblastic markers (our unpublished data). When transplanted into immunocompromised mice these cells form a cementum-like tissue with ECM characteristic of the cementum [86]. These observations underscore the crucial role played by the microenvironment in the selective recruitment of stem/progenitor cells, their expansion and subsequent differentiation [65, 66, 73•].…”

Section: Stem Cell Niche and Stem Cell Differentiationmentioning

confidence: 92%

Periodontal Stem Cells: a Historical Background and Current Perspectives

Pitaru¹,

Narayanan

Etikala

et al. 2013

Curr Oral Health Rep

Self Cite

View full text Add to dashboard Cite

In this review we discuss the historical perspective of stem cell populations from oral tissues in light of our current understanding of stem cell biology. Stem cells and their niches have been identified in the periodontium starting from the late 1970s. Applying new criteria for the identification and characterization reveals that oral tissues comprise a multipotent primitive neural crest-like stem cell population capable of differentiating into neural crest derived cell lineages of the cranial-facial zone. This population supplies cells to a more restricted stem cell type with tissue specific epigenetic memory that differentiates into cell lineages characteristic of their tissue origin. We believe that the microenvironment plays an essential role in maintaining stem cell populations and directing their migration and differentiation, and that this factor needs to be considered for utilization of stem cell-based therapy for periodontal regeneration and regenerative dental medicine.

show abstract

Section: Stem Cell Niche and Stem Cell Differentiationmentioning

confidence: 92%

Periodontal Stem Cells: a Historical Background and Current Perspectives

Pitaru¹,

Narayanan

Etikala

et al. 2013

Curr Oral Health Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…In contrast to the extensive study on stem/ progenitor cells from the mesenchymal compartment of dental pulp and periodontal ligament [28], little is known about the presence or functions of endothelial stem/progenitor cell populations in oral mucosa. Recent reports have demonstrated that the gingival mucosa comprised a population of multipotent stem cells that express characteristic mesenchymal stromal cell (MSC) markers and can differentiate into osteogenic, chondroblastic, and adipogenic lineages [29][30][31][32][33][34]. We hypothesize that oral mucosa carries EPCs at a higher level than lung, PB, or BM and possesses angiogenic potential in vascular regeneration.…”

Section: Cd133mentioning

confidence: 94%

Oral Mucosa Harbors a High Frequency of Endothelial Cells: A Novel Postnatal Cell Source for Angiogenic Regeneration

Zhou

Rogers

Lee

et al. 2017

Stem Cells and Development

View full text Add to dashboard Cite

Endothelial progenitor cells/endothelial cells (EPCs/ECs) have great potential to treat pathological conditions such as cardiac infarction, muscle ischemia, and bone fractures, but isolation of EPC/ECs from existing cell sources is challenging due to their low EC frequency. We have isolated endothelial progenitor (EP)-like cells from rat oral mucosa and characterized their yield, immunophenotype, growth, and in vivo angiogenic potential. The frequency of EP-like cells derived from oral mucosa is thousands of folds higher than EPCs derived from donor-match bone marrow samples. EP-like cells from oral mucosa were positive for EC markers CD31, VECadherin, and VEGFR2. Oral mucosa-derived EP-like cells displayed robust uptake of acetylated low-density lipoprotein and formed stable capillary networks in Matrigel. Subcutaneously implanted oral mucosa-derived EP-like cells anastomosed with host blood vessels, implicating their ability to elicit angiogenesis. Similar to endothelial colony-forming cells, EP-like cells from oral mucosa have a significantly higher proliferative rate than human umbilical vein endothelial cells. These findings identify a putative EPC source that is easily accessible in the oral cavity, potentially from discarded tissue specimens, and yet with robust yield and potency for angiogenesis in tissue and organ regeneration.

show abstract

“…Out of which 5 studies were regarding regeneration of cementum or cementum like tissue [104], 4 studies attempted regeneration of the cementum-PDL complex [105] and 1 study cementum and bone [106].…”

Section: Cementum Regenerationmentioning

confidence: 99%

The Current Status of Stem Cell Regeneration in Intra Oral Applications—A Systematic Review

Kanwal¹,

D’souza²,

Muthiah³

et al. 2017

OJST

View full text Add to dashboard Cite

Aim: 1) To review the literature of various applications of stem cell regeneration in dentistry from 2010 to 2016. 2) To review these studies and to summarize the current status of stem cell regeneration in intra oral applications.3) To present the available successful data with regard to stem cell regeneration in dentistry and to highlight the future trends. Materials and Methods: Search Protocol: A systematic search was made in the PubMed database with the key words gingiva, papillary reconstruction, periodontal ligament, dental pulp, salivary gland, enamel re-mineralization, dentin, cementum, bone, whole tooth, cleft palate, regeneration and stem cells. All articles from 2010 to 2016, relevant to the topic were included. After the search a total of 1826 articles were obtained which were screened and categorized by three independent reviewers as review papers, in vitro, animal and human studies, pertaining to stem cell regeneration in intra oral applications. On the basis of the extracted data and outcome measures, conclusions were drawn. Results: Maximum number of animal studies has been done to regenerate periodontal tissues, bone, dentin and pulp tissues. Few human studies have been done till date. Although clinically, researchers have been able to regenerate periodontal-like tissue, bone and pulp-like tissue, they still haven't been able to regenerate tissues that completely resemble tissues in their natural form. Conclusion: The future of stem cell therapy in dental applications looks promising. The predictability and efficacy of outcomes, as well as safety concerns of stem cell therapy is yet to be fully established. Ongoing research and development of newer scaffolds, understanding various signaling molecules and their cues, understanding gene expression and proteomics of stem cells are the future directions that will take us a step forward to achieving successful regeneration.

show abstract

Stem cells of the lamina propria of human oral mucosa and gingiva develop into mineralized tissues in vivo

Abstract: Within the limitation of this study, the results indicate for the first time that fibrin-hOMSC constructs are endowed with the constitutive capacity to develop into mineralized tissues that exhibit certain similarities to cementum and bone.

Cited by 38 publications

References 37 publications

Periodontal Stem Cells: a Historical Background and Current Perspectives

Periodontal Stem Cells: a Historical Background and Current Perspectives

Oral Mucosa Harbors a High Frequency of Endothelial Cells: A Novel Postnatal Cell Source for Angiogenic Regeneration

The Current Status of Stem Cell Regeneration in Intra Oral Applications—A Systematic Review

Contact Info

Product

Resources

About