Stem cells combined with bone graft substitutes in skeletal tissue engineering

Gamie, Zakareya; Tran, Gui; Vyzas, George; Korres, Nectarios; Heliotis, Manolis; Mantalaris, Athanasios; Tsiridis, Eleftherios

doi:10.1517/14712598.2012.679652

Cited by 74 publications

(62 citation statements)

References 133 publications

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“…102 Gamie et al 102 provided a comprehensive review of various sources of stem cells in combination with bone graft substitutes for bone tissue engineering through osteogenic differentiation. Sources of these cells range from ESCs to an array of cells from adult mesenchymal origin, including umbilical cord stem cells, bone marrow and periosteum-derived stem cells, adipose-derived stem cells (ADSCs), synovium-derived stem cells (SDSCs), and, more recently, induced pluripotent stem cells (iPSCs) were shown to lead to osteogenesis both in vitro and in vivo.…”

Section: Incorporating Scbts Into the Designmentioning

confidence: 99%

“…Sources of these cells range from ESCs to an array of cells from adult mesenchymal origin, including umbilical cord stem cells, bone marrow and periosteum-derived stem cells, adipose-derived stem cells (ADSCs), synovium-derived stem cells (SDSCs), and, more recently, induced pluripotent stem cells (iPSCs) were shown to lead to osteogenesis both in vitro and in vivo. 41,102 Cells from these sources possess some degree of pluripotency, where differentiation can lead to a number of distinct cell types, including osteoblasts and chondrocytes.…”

Section: Incorporating Scbts Into the Designmentioning

confidence: 99%

See 1 more Smart Citation

Endochondral Ossification for Enhancing Bone Regeneration: Converging Native Extracellular Matrix Biomaterials and Developmental Engineering In Vivo

Dennis

Berkland

Bonewald

et al. 2015

Tissue Engineering Part B: Reviews

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Section: Incorporating Scbts Into the Designmentioning

confidence: 99%

Section: Incorporating Scbts Into the Designmentioning

confidence: 99%

Endochondral Ossification for Enhancing Bone Regeneration: Converging Native Extracellular Matrix Biomaterials and Developmental Engineering In Vivo

Dennis

Berkland

Bonewald

et al. 2015

Tissue Engineering Part B: Reviews

View full text Add to dashboard Cite

“…Mesenchymal stem cell differentiation can be viewed as: 1) as a recapitulation of early developmental processes and 2) as a means of regenerating tissue following injury [15,34]. Both processes are highly affected by altered matricellular expression [23][24][25]; therefore, our results indicate that DPT may doi: 10.7243/2054-720X-1-2 play a vital role in skeletal wound healing as a matricellular protein.…”

Section: Discussionmentioning

confidence: 81%

“…However, skeletal ECM has also been shown to have a beneficial effect on osteogenesis. Much of the published work concerning the effect of individual ECM components on osteogenesis has centered on the more abundant matrix proteins and on inorganic ECM components common in mineralized tissue [15][16][17][18][19]. Also commonly studied are the influences of synthetic ECM-mimicking substrates and various growth factors [15,[20][21][22].…”

Section: Discussionmentioning

confidence: 99%

Dermatopontin in the extracellular matrix enhances osteogenic differentiation of adipose-derived mesenchymal stem cells

Coan¹,

Lively²,

Dyke³

2014

Musculoskelet Biol

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Background: Dermatopontin (DPT) is a 22-kDa tyrosine-rich extracellular matrix (ECM) protein that is found at high levels in demineralized bone matrix and cartilage and may play an important role in skeletal tissue function. In the current study, we investigate whether DPT in the ECM plays a role in the enhanced osteogenic differentiation of adipose-derived stem cells (ADSCs). Methods: In order to determine whether DPT modulates osteogenesis, we overexpressed the DPT gene in ADSC using stable lentiviral infection, induced the DPT-overexpressing cells to differentiate, and isolated the ECM secreted by these cells during osteogenesis. Using the secreted ECM with "higher than normal" levels of DPT embedded within as a substrate for cell growth, we assessed the extent to which the excess DPT modulated osteogenic marker expression during osteogenic differentiation of naive ADSC. Results: We found that ADSC cultured on the DPT-enriched ECM differentiated towards an osteogenic phenotype more robustly, as measured by expression of osteogenic marker genes. Conclusions: This may indicate an important role for DPT in the induction of stem cells toward an osteoblast phenotype during skeletal wound healing. DPT may present a novel candidate for future studies of stem cell osteogenesis and the development of more biologically relevant biomaterial substrates for bone regeneration.

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“…Embedding viable cells within the biological scaffolds appears to be extremely promising since it allows osteocompetent cells to generate new bone tissue and contribute to the improved tissue healing (Connolly et al, 1989;Gamie et al, 2012;Giannotti et al, 2013a). Mesenchymal stem cells (MSCs) represent a good candidate cell source because of their biological characteristics and potential role for clinical bone regeneration (Chanda et al, 2010;Jäger et al, 2011;Kuroda et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Autologous Bone Marrow Stem Cells combined with Allograft Cancellous Bone in Treatment of Nonunion

et al. 2015

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Abstract-Autologous cancellous bone graft is currently used as a gold standard method for treatment of bone nonunion. However, there is a limit to the amount of autologous cancellous bone that can be harvested and the donor site morbidity presents a major disadvantage to autologous bone grafting. Embedding viable cells within biological scaffolds appears to be extremely promising. The purpose of this study was to assess the outcome of autologous bone marrow stem cells combined with a cancellous bone allograft as compared to an autologous bone graft in the treatment of bone nonunion. Bone marrow aspiration concentrate (BMAC) was previously produced from bone marrow aspirate via a density gradient centrifugation. Autologous cancellous bone was harvested in 9 patients and applied to the nonunion site. In 18 patients of the clinical trial group after the debridement, the bone gaps were filled with a composite of BMAC and allograft cancellous bone chips (BMAC-ACB). Bone consolidation was obtained in 88.9%, and the mean interval between the cell transplantation and union was 4.6 ± 1.5 months in the autograft group. Bone union rate was 94.4% in group of composite BMAC-ACB implantation. The time to union in BMAC-ACB grafting group was 3.3 ± 0.90 months, and led to faster healing when compared to the autograft. A mean concentration of autologous progenitorcells was found to be 2.43 ± 1.03 (x10 6 ) CD34 + cells/ml, and a mean viability of CD34 + cells was 97.97 ± 1.47 (%). This study shows that the implantation of BMAC has presented the efficacy for treatment of nonunion and may contribute an available alternative to autologous cancellous bone graft. But large clinical application of BM-MSCs requires a more appropriate and profound scientific investigations.

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Stem cells combined with bone graft substitutes in skeletal tissue engineering

Cited by 74 publications

References 133 publications

Endochondral Ossification for Enhancing Bone Regeneration: Converging Native Extracellular Matrix Biomaterials and Developmental Engineering In Vivo

Endochondral Ossification for Enhancing Bone Regeneration: Converging Native Extracellular Matrix Biomaterials and Developmental Engineering In Vivo

Dermatopontin in the extracellular matrix enhances osteogenic differentiation of adipose-derived mesenchymal stem cells

Autologous Bone Marrow Stem Cells combined with Allograft Cancellous Bone in Treatment of Nonunion

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