Stem cells applications in bone and tooth repair and regeneration: New insights, tools, and hopes

Meguid, Eiman Abdel; Ke, Yuehai; Ji, Junfeng; El-Hashash, Ahmed

doi:10.1002/jcp.25940

Cited by 60 publications

(39 citation statements)

References 84 publications

(99 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Hence, this study demonstrates that the bottleneck of bioengineered tooth innervation can be circumvented by using innovative antagonist of NRP1. While strengthening the demonstration of the proof of concept of a TM domain targeting peptides as useful drugs in vivo, our results contribute to the development of mounting tooth repair strategies (Abdel Meguid, Ke, Ji, & El‐Hashash, ).…”

Section: Resultssupporting

confidence: 58%

Semaphorin 3A receptor inhibitor as a novel therapeutic to promote innervation of bioengineered teeth

Kuchler‐Bopp

Bagnard

Van‐Der‐Heyden

et al. 2018

J Tissue Eng Regen Med

View full text Add to dashboard Cite

The sensory innervation of the dental pulp is essential for tooth function and protection. It is mediated by axons originating from the trigeminal ganglia and is spatio-temporally regulated. We have previously shown that the innervation of bioengineered teeth can be achieved only under immunosuppressive conditions. The aim of this study was to develop a model to determine the role of Semaphorin 3A (Sema3A) in the innervation of bioengineered teeth. We first analysed innervation of the dental pulp of mandibular first molars in newborn (postnatal day 0: PN0) mice deficient for Sema3A (Sema3A ), a strong inhibitor of axon growth. While at PN0, axons detected by immunostaining for peripherin and NF200 were restricted to the peridental mesenchyme in Sema3A mice, they entered the dental pulp in Sema3A mice. Then, we have implanted cultured teeth obtained from embryonic day-14 (E14) molar germs of Sema3A mice together with trigeminal ganglia. The dental pulps of E14 cultured and implanted Sema3A teeth were innervated, whereas the axons did not enter the pulp of E14 Sema3A cultured and implanted teeth. A "Membrane Targeting Peptide NRP1," suppressing the inhibitory effect of Sema3A, has been previously identified. The injection of this peptide at the site of implantation allowed the innervation of the dental pulp of bioengineered teeth obtained from E14 dental dissociated mesenchymal and epithelial cells reassociations of ICR mice. In conclusion, these data show that inhibition of only one axon repellent molecule, Sema3A, allows for pulp innervation of bioengineered teeth.

show abstract

Section: Resultssupporting

confidence: 58%

Semaphorin 3A receptor inhibitor as a novel therapeutic to promote innervation of bioengineered teeth

Kuchler‐Bopp

Bagnard

Van‐Der‐Heyden

et al. 2018

J Tissue Eng Regen Med

View full text Add to dashboard Cite

show abstract

“…There are several mesenchymal stem cells (MSCs) sources available for clinical applications. Human umbilical cord mesenchymal stem cells (HUCMSCs) are MSCs found in the umbilical cords and may be a prospective candidate for use in tissue regeneration [1]. Compared to other sources of stem cells from adult tissues, HUCMSCs are more primitive, non-invasive in terms of collection, provide high proliferation potential, high differentiation potential, immune-privileged, immunosuppressive, and rich in stemness [2].…”

Section: Introductionmentioning

confidence: 99%

Lipopolysaccharide’s Cytotoxicity on Human Umbilical Cord Mesenchymal Stem Cells

Kuntjoro

Prasetyo

Cahyani

et al. 2020

Pesqui. Bras. Odontopediatria Clín. Integr.

View full text Add to dashboard Cite

To show the cytotoxicity of Porphyromonas gingivalis lipopolysaccharide (LPS) on human umbilical cord mesenchymal stem cells (HUCMSCs) to better understand the characteristics for its application in regenerative procedures under periodontopathogen LPS influence. Material and Methods: Ultrapure Porphyromonas gingivalis LPS was used in this study. This research used a frozen stock HUCMSCs, previously confirmed by flow cytometry. The biological characteristics, such as cell morphology, proliferation, and protein expression, were screened. To check the cytotoxicity, HUCMSCs were cultured and divided into two groups, the control group and LPS group with various concentrations from 25 to 0.39 µg/mL. MTT assay was done and the cells were observed and counted. The significance level was set at 5%. Results: The percentage of living HUCMSCs on LPS group were not significantly different among concentrations (p>0.05) from 25 to 0.39 µg/mL, even though there were slight mean decrease between groups, but they were not significant. The duration of 24 hours of exposure of LPS does not significantly lower HUCMSCs viability. Conclusion: LPS does not affect the viability of HUCMSCs. The lower the concentration of LPS, the higher the viability of HUCMSCs.

show abstract

“…There are several mesenchymal stem cells (MSC) sources for clinical applications. Human umbilical cord mesenchymal stem cells (HUCMSCs) are MSC found in the umbilical cords and may be attractive candidates for tissue regeneration [1]. Compared to other sources of stem cells from adult tissues, HUCMSCs are more primitive, non-invasive procedure of collection, provide high proliferation potential, high differentiation potential, immune-privileged, immunosuppressive, rich in stemness [2].…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity of Calcium Hydroxide on Human Umbilical Cord Mesenchymal Stem Cells

Prasetyo

Widjiastuti

Cahyani

et al. 2020

Pesqui. Bras. Odontopediatria Clín. Integr.

View full text Add to dashboard Cite

To examine the cytotoxicity of calcium hydroxide on human umbilical cord mesenchymal stem cells (HUCMSC) to understand the characteristics for use in regenerative dentistry procedures especially regenerative endodontics. Material and Methods: HUCMSC was isolated, cultured, and confirmed by flow cytometry. The biological characteristics, such as cell morphology, proliferation, and protein expression, were screened. To check the cytotoxicity, HUCMSC was cultured and divided into two groups, the control group (cultured in minimum essential medium (MEM) alpha) and calcium hydroxide group (cultured in MEM alpha and calcium hydroxide). Methyl-thiazole-tetrazolium (MTT) assay was done on different concentrations of calcium hydroxide (0.39 to 25 µg/mL) and the cells were observed and counted. One-way ANOVA test was used with a significance level set at 5%. Results: Flow cytometric analysis confirmed positive of CD73, CD90, CD105, negative of CD45 and CD34. A significant difference was found between the concentration of 6.25 and 3.125 µg/mL (p=0.004). There was no significant difference among 6.25, 12.5 and 25 µg/mL concentrations. There was also no significant difference among 0.39, 0.78, 1.56, and 3.125 µg/mL concentrations. Conclusion: Even though calcium hydroxide is a medicament of choice in clinical endodontics, it decreases the viability of HUCMSC. The lower the concentration of calcium hydroxide, the higher the viability of HUCMSC.

show abstract

Stem cells applications in bone and tooth repair and regeneration: New insights, tools, and hopes

Cited by 60 publications

References 84 publications

Semaphorin 3A receptor inhibitor as a novel therapeutic to promote innervation of bioengineered teeth

Semaphorin 3A receptor inhibitor as a novel therapeutic to promote innervation of bioengineered teeth

Lipopolysaccharide’s Cytotoxicity on Human Umbilical Cord Mesenchymal Stem Cells

Cytotoxicity of Calcium Hydroxide on Human Umbilical Cord Mesenchymal Stem Cells

Contact Info

Product

Resources

About