Lipopolysaccharide’s Cytotoxicity on Human Umbilical Cord Mesenchymal Stem Cells

Kuntjoro, Mefina; Prasetyo, Eric Priyo; Cahyani, Febriastuti; Kamadjaja, Michael Josef Kridanto; Hendrijantini, Nike; Laksono, Harry; Rahmania, Primanda Nur; Ariestania, Vivin; Nugraha, Alexander Patera; Ihsan, Igo Syaiful; Dinaryanti, Aristika; Rantam, Fedik Abdul

doi:10.1590/pboci.2020.153

Cited by 10 publications

(13 citation statements)

References 25 publications

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“…28 The other sources of MSCs, such as those derived from hair follicles, the umbilical cord, adipose tissue, and dental pulp, also positively presented the specific cell surface markers of MSCs. 5,6,8,9,11 GDMSCs have a potential proliferation rate characterized by Oct-4, Nanog, and SOX2 expression that play an important role in the self-renewal and survival of MSCs. 41,42 In addition to the proliferation potential of GDMSCs, their migration capability is also good.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…3 MSCs have been successfully extracted from various tissues, such as the umbilical cord, hair follicles, and adipose tissue, including orofacial tissues. [4][5][6][7][8] MSCs can be derived from orofacial tissue, including the dental pulp of permanent or deciduous teeth, the periodontal ligament, and the gingiva. [9][10][11][12][13] One of the potential and superior sources of MSCs from the oral cavity was in the gingiva.…”

Section: Introductionmentioning

confidence: 99%

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Gingival-Derived Mesenchymal Stem Cell from Rabbit (Oryctolagus cuniculus): Isolation, Culture, and Characterization

et al. 2020

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Objective This study aims to confirm whether the GDMSCs isolated from rabbit’s (Oryctolagus cuniculus) gingiva are mesenchymal stem cells (MSCs). Materials and Methods This study design was partly quasi-experimental with an observational design. GDMSCs were isolated from the gingiva of healthy male rabbits (O. cuniculus) (n = 2), 6 months old, and 3 to 5 kg of body weight. The specific cell surface markers of MSCs; clusters of differentiation (CD), namely, CD44, CD73, CD90, CD105, and CD200 expressions; and hematopoietic stem cell surface markers CD34 and CD45 were examined using flow cytometry and immunohistochemistry with immunofluorescence. The osteogenic differentiation of isolated GDMSCs was examined using alizarin red staining. Results GDMSCs in the fourth passage showed a spindle-like formation and fibroblast-like cells that attached to the base of the culture plate. GDMSCs were MSCs that positively expressed CD44, CD73, CD90, CD105, and CD200 but did not express CD34 and CD45 when examined using flow cytometry and immunohistochemical analysis. GDMSCs had osteogenic differentiation confirmed by calcified deposits in vitro with a red–violet and brownish color after alizarin red staining. Conclusion GDMSCs isolated from the rabbits (O. cuniculus) were confirmed as MSCs in vitro documented using immunohistochemistry and flow cytometry. GDMSCs can differentiate into osteogenic lineage in vitro that may be suitable for regenerative dentistry.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Gingival-Derived Mesenchymal Stem Cell from Rabbit (Oryctolagus cuniculus): Isolation, Culture, and Characterization

et al. 2020

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“…This research sample used various scaffold HA-TCP doses with three sample replications in each group, following previous research using HUCMSCs [21]. Data being counted was based on standard deviation and average.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of HA-TCP Scaffold on Human Umbilical Cord Mesenchymal Stem Cells using MTT Assay

Ariestania

Hendrijantini

Prahasanti

et al. 2022

IJIE

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Hydroxyapatite-tricalcium phosphate (HA-TCP) scaffold is a three-dimensional structure used to support bone regeneration. Ideally, the scaffold should be biocompatible, biodegradable, and non-toxic. The tissue engineering technique uses a combined stem cell and scaffold to repair the bone defect. To prove the non-toxic properties of the scaffold, a cytotoxicity test is needed for the human umbilical cord mesenchymal stem cell (HUCMSCs). In this study, 27 samples were divided into eight groups with a variant of scaffold HA-TCP doses ranging from 5–1000 µg. Each treatment group of scaffolds was covered in HUCMSCs. The samples were added by methyl-thiazol-tetrazolium (MTT) colorimetric assay, counted using an optical density (OD) formula, and observed by a microplate reader. The viability of the cells was observed by an inverted TMS microscope with 100x magnification. The MTT assay’s test shows that the HUCMSCs cell viability shed each variant of HA-TCP scaffold dose, did not show any toxic effect. The higher the OD value, the higher the viability. It has been found that there is no significant difference between the variable scaffold doses to the percentage viability of umbilical cord cells.

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“…The thawed HUCMSCs were expanded and ready to be used for further study. 11 Calcium hydroxide was obtained by combining minimum essential medium alpha (Gibco, UK) with the powder (EMSURE Merck, Germany). Calcium hydroxide concentration of 0.1 microgram/mL was used in this experiment.…”

Section: Preparation Of Hucmscs and Calcium Hydroxidementioning

confidence: 99%

Calcium Hydroxide Increases Human Umbilical Cord Mesenchymal Stem Cells Expressions of Apoptotic Protease-Activating Factor-1, Caspase-3 and Caspase-9

Prasetyo

Kuntjoro

Goenharto

et al. 2021

CCIDE

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Purpose Calcium hydroxide is a gold standard dental material generally used for pulpal and periapical therapy including regenerative endodontic procedures because of its positive properties. However, evaluation about this material on stem cells is limited. Human umbilical cord mesenchymal stem cells (HUCMSCs) are potential to be used in regenerative therapy. Regenerative therapy needs a sustainable cell supply to maintain its regenerative capacity. The aim of this study was to ascertain the apoptosis result of calcium hydroxide on HUCMSCs through the expression of apoptotic protease-activating factor-1 (APAF-1), caspase-3, and caspase-9. Materials and Methods This study used a thawed frozen stock of passage 5 HUCMSCs, grown in minimum essential medium (MEM) alpha containing calcium hydroxide at concentration of 0.1 microgram/mL for 1, 3 and 7 days. Polyclonal antibody with fluorescence isothiocyanate (FITC) label was used to evaluate the expressions. APAF-1, caspase-3, and caspase-9 expressions were recorded and compared on every observation day using fluorescence microscope. Analysis of variance was performed to analyze the significance among the results of treatment groups. The results were concluded significant if p<0.05. Results The addition of calcium hydroxide in MEM alpha medium increases HUCMSCs expression of APAF-1, caspase-3 and caspase-9 significantly, compared to the control group without calcium hydroxide (p<0.05) in all the times. Day 1 showed the lowest increase followed by higher expressions on day 3 and day 7. Conclusion HUCMSCs express increased APAF-1, caspase-3 and caspase-9 after in-vitro calcium hydroxide exposure. This should be considered when using calcium hydroxide on HUCMSCs for regenerative procedures with regard to other positive properties.

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Lipopolysaccharide’s Cytotoxicity on Human Umbilical Cord Mesenchymal Stem Cells

Cited by 10 publications

References 25 publications

Gingival-Derived Mesenchymal Stem Cell from Rabbit (Oryctolagus cuniculus): Isolation, Culture, and Characterization

Gingival-Derived Mesenchymal Stem Cell from Rabbit (Oryctolagus cuniculus): Isolation, Culture, and Characterization

Cytotoxicity of HA-TCP Scaffold on Human Umbilical Cord Mesenchymal Stem Cells using MTT Assay

Calcium Hydroxide Increases Human Umbilical Cord Mesenchymal Stem Cells Expressions of Apoptotic Protease-Activating Factor-1, Caspase-3 and Caspase-9

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